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74 results on '"Baleeva, Nadezhda S."'

53. Red‐Shifted Substrates for FAST Fluorogen‐Activating Protein Based on the GFP‐Like Chromophores

Author

Povarova, Natalia V., primary, Zaitseva, Snizhana O., additional, Baleeva, Nadezhda S., additional, Smirnov, Alexander Yu., additional, Myasnyanko, Ivan N., additional, Zagudaylova, Marina B., additional, Bozhanova, Nina G., additional, Gorbachev, Dmitriy A., additional, Malyshevskaya, Kseniya K., additional, Gavrikov, Alexey S., additional, Mishin, Alexander S., additional, and Baranov, Mikhail S., additional

Published
2019
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57. Convenient and Versatile Synthetic Protocol for Arylidene‐1H‐imidazol‐5(4H)‐ones.

Author

Sokolov, Anatolii I., Myasnyanko, Ivan N., Baleeva, Nadezhda S., and Baranov, Mikhail S.

Subjects
GREEN fluorescent protein, CHROMOPHORES synthesis, RING formation (Chemistry), SCHIFF bases, OPTICAL properties, CHROMOPHORES
Abstract

We propose a new protocol for synthesis of the arylidene‐1H‐imidazol‐5(4H)‐ones which is based on O‐alkylation of amidoacetates using triethyloxonium tetrafluoroborate leading to carboximidates. These imidates have long been used in the synthesis of imidazolones by [2+3] cycloaddition reaction with Schiff bases. However, diversity of carboximidates prepared by the common techniques was limited. The protocol we proposed eliminated these limitations. Using this technique, a number of derivatives of the green fluorescent protein (GFP) and asFP chromophores were synthesized, and influence of the C2 residue on their optical properties was studied. [ABSTRACT FROM AUTHOR]

Published
2020
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65. Mechanism and color modulation of fungal bioluminescence

Author

Kaskova, Zinaida M., primary, Dörr, Felipe A., additional, Petushkov, Valentin N., additional, Purtov, Konstantin V., additional, Tsarkova, Aleksandra S., additional, Rodionova, Natalja S., additional, Mineev, Konstantin S., additional, Guglya, Elena B., additional, Kotlobay, Alexey, additional, Baleeva, Nadezhda S., additional, Baranov, Mikhail S., additional, Arseniev, Alexander S., additional, Gitelson, Josef I., additional, Lukyanov, Sergey, additional, Suzuki, Yoshiki, additional, Kanie, Shusei, additional, Pinto, Ernani, additional, Di Mascio, Paolo, additional, Waldenmaier, Hans E., additional, Pereira, Tatiana A., additional, Carvalho, Rodrigo P., additional, Oliveira, Anderson G., additional, Oba, Yuichi, additional, Bastos, Erick L., additional, Stevani, Cassius V., additional, and Yampolsky, Ilia V., additional

Published
2017
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67. Azidoacetic Acid Amides in the Synthesis of Substituted Arylidene‐1‐H‐imidazol‐5‐(4H)‐ones.

Author

Zaitseva, Snizhana O., Golodukhina, Svetlana V., Baleeva, Nadezhda S., Levina, Evgenia A., Smirnov, Alexander Yu., Zagudaylova, Marina B., and Baranov, Mikhail S.

Abstract

Abstract: We present a novel efficient method for the synthesis of substituted arylidene‐1‐H‐imidazol‐5‐(4H)‐ones based on the use of azidoacetic acid amides. The proposed approach was successfully used for the preparation of three rows of target substances with different substituents in the imidazolone ring and benzylidene moiety. The presented methodology tends to be multipurpose and convenient for the introduction of substituents in any position. [ABSTRACT FROM AUTHOR]

Published
2018
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71. Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins.

Author

Bozhanova, Nina G., Baranov, Mikhail S., Baleeva, Nadezhda S., Gavrikov, Alexey S., and Mishin, Alexander S.

Subjects
GREEN fluorescent protein, CHROMOPHORES, CELL imaging, LIPOCALINS, PROTEIN synthesis
Abstract

Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs. [ABSTRACT FROM AUTHOR]

Published
2018
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72. Developing Bright Green Fluorescent Protein (GFP)-like Fluorogens for Live-Cell Imaging with Nonpolar Protein-Chromophore Interactions.

Author

Chen C, Tachibana SR, Baleeva NS, Myasnyanko IN, Bogdanov AM, Gavrikov AS, Mishin AS, Malyshevskaya KK, Baranov MS, and Fang C

Subjects
Green Fluorescent Proteins genetics, Microscopy, Fluorescence, Fluorescent Dyes
Abstract

Fluorescence-activating proteins (FAPs) that bind a chromophore and activate its fluorescence have gained popularity in bioimaging. The fluorescence-activating and absorption-shifting tag (FAST) is a light-weight FAP that enables fast reversible fluorogen binding, thus advancing multiplex and super-resolution imaging. However, the rational design of FAST-specific fluorogens with large fluorescence enhancement (FE) remains challenging. Herein, a new fluorogen directly engineered from green fluorescent protein (GFP) chromophore by a unique double-donor-one-acceptor strategy, which exhibits an over 550-fold FE upon FAST binding and a high extinction coefficient of approximately 100,000 M -1  cm -1 , is reported. Correlation analysis of the excited state nonradiative decay rates and environmental factors reveal that the large FE is caused by nonpolar protein-fluorogen interactions. Our deep insights into structure-function relationships could guide the rational design of bright fluorogens for live-cell imaging with extended spectral properties such as redder emissions., (© 2021 Wiley-VCH GmbH.)

Published
2021
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73. Red-Shifted Substrates for FAST Fluorogen-Activating Protein Based on the GFP-Like Chromophores.

Author

Povarova NV, Zaitseva SO, Baleeva NS, Smirnov AY, Myasnyanko IN, Zagudaylova MB, Bozhanova NG, Gorbachev DA, Malyshevskaya KK, Gavrikov AS, Mishin AS, and Baranov MS

Subjects
Cell Nucleus ultrastructure, Fluorescence, HEK293 Cells, HeLa Cells, Humans, Microscopy, Confocal, Microscopy, Fluorescence, Optical Imaging, Fluorescent Dyes chemistry, Green Fluorescent Proteins chemistry, Rhodanine analogs & derivatives
Abstract

A genetically encoded fluorescent tag for live cell microscopy is presented. This tag is composed of previously published fluorogen-activating protein FAST and a novel fluorogenic derivative of green fluorescent protein (GFP)-like chromophore with red fluorescence. The reversible binding of the novel fluorogen and FAST is accompanied by three orders of magnitude increase in red fluorescence (580-650 nm). The proposed dye instantly stains target cellular proteins fused with FAST, washes out in a minute timescale, and exhibits higher photostability of the fluorescence signal in confocal and widefield microscopy, in contrast with previously published fluorogen:FAST complexes., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2019
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74. Red-shifted fluorescent aminated derivatives of a conformationally locked GFP chromophore.

Author

Baranov MS, Solntsev KM, Baleeva NS, Mishin AS, Lukyanov SA, Lukyanov KA, and Yampolsky IV

Subjects
Amination, Fluorescent Dyes metabolism, Green Fluorescent Proteins chemistry, Green Fluorescent Proteins genetics, HeLa Cells, Humans, Hydrogen-Ion Concentration, Microscopy, Confocal, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Solvents chemistry, Spectrometry, Fluorescence, Fluorescent Dyes chemistry, Green Fluorescent Proteins metabolism
Abstract

A novel class of fluorescent dyes based on conformationally locked GFP chromophore is reported. These dyes are characterized by red-shifted spectra, high fluorescence quantum yields and pH-independence in physiological pH range. The intra- and intermolecular mechanisms of radiationless deactivation of ABDI-BF2 fluorophore by selective structural locking of various conformational degrees of freedom were studied. A unique combination of solvatochromic and lipophilic properties together with "infinite" photostability (due to a dynamic exchange between free and bound dye) makes some of the novel dyes promising bioinspired tools for labeling cellular membranes, lipid drops and other organelles., (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2014
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