Back to Search
Start Over
Developing Bright Green Fluorescent Protein (GFP)-like Fluorogens for Live-Cell Imaging with Nonpolar Protein-Chromophore Interactions.
- Source :
-
Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2021 Jun 21; Vol. 27 (35), pp. 8946-8950. Date of Electronic Publication: 2021 May 21. - Publication Year :
- 2021
-
Abstract
- Fluorescence-activating proteins (FAPs) that bind a chromophore and activate its fluorescence have gained popularity in bioimaging. The fluorescence-activating and absorption-shifting tag (FAST) is a light-weight FAP that enables fast reversible fluorogen binding, thus advancing multiplex and super-resolution imaging. However, the rational design of FAST-specific fluorogens with large fluorescence enhancement (FE) remains challenging. Herein, a new fluorogen directly engineered from green fluorescent protein (GFP) chromophore by a unique double-donor-one-acceptor strategy, which exhibits an over 550-fold FE upon FAST binding and a high extinction coefficient of approximately 100,000 M <superscript>-1</superscript> cm <superscript>-1</superscript> , is reported. Correlation analysis of the excited state nonradiative decay rates and environmental factors reveal that the large FE is caused by nonpolar protein-fluorogen interactions. Our deep insights into structure-function relationships could guide the rational design of bright fluorogens for live-cell imaging with extended spectral properties such as redder emissions.<br /> (© 2021 Wiley-VCH GmbH.)
- Subjects :
- Green Fluorescent Proteins genetics
Microscopy, Fluorescence
Fluorescent Dyes
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3765
- Volume :
- 27
- Issue :
- 35
- Database :
- MEDLINE
- Journal :
- Chemistry (Weinheim an der Bergstrasse, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 33938061
- Full Text :
- https://doi.org/10.1002/chem.202101250