51. Designing a multi-epitope peptide based vaccine against SARS-CoV-2.
- Author
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Singh A, Thakur M, Sharma LK, and Chandra K
- Subjects
- Antigen-Antibody Complex chemistry, Antigen-Antibody Complex immunology, Antigens, Viral immunology, B-Lymphocytes immunology, COVID-19 Vaccines, Coronavirus Infections immunology, Coronavirus Infections prevention & control, Coronavirus Nucleocapsid Proteins, Epitopes chemistry, HLA Antigens chemistry, HLA Antigens immunology, Humans, Immunogenicity, Vaccine, Nucleocapsid Proteins chemistry, Nucleocapsid Proteins immunology, Phosphoproteins, Spike Glycoprotein, Coronavirus chemistry, Spike Glycoprotein, Coronavirus immunology, T-Lymphocytes immunology, Toll-Like Receptors immunology, Vaccines, Subunit chemistry, Viral Matrix Proteins chemistry, Viral Matrix Proteins immunology, Viral Vaccines chemistry, Epitopes immunology, Molecular Docking Simulation, Vaccines, Subunit immunology, Viral Vaccines immunology
- Abstract
COVID-19 pandemic has resulted in 16,114,449 cases with 646,641 deaths from the 217 countries, or territories as on July 27th 2020. Due to multifaceted issues and challenges in the implementation of the safety and preventive measures, inconsistent coordination between societies-governments and most importantly lack of specific vaccine to SARS-CoV-2, the spread of the virus that initially emerged at Wuhan is still uprising after taking a heavy toll on human life. In the present study, we mapped immunogenic epitopes present on the four structural proteins of SARS-CoV-2 and we designed a multi-epitope peptide based vaccine that, demonstrated a high immunogenic response with a vast application on world's human population. On codon optimization and in-silico cloning, we found that candidate vaccine showed high expression in E. coli and immune simulation resulted in inducing a high level of both B-cell and T-cell mediated immunity. The results predicted that exposure of vaccine by administrating three injections significantly subsidized the antigen growth in the system. The proposed candidate vaccine found promising by yielding desired results and hence, should be validated by practical experimentations for its functioning and efficacy to neutralize SARS-CoV-2.
- Published
- 2020
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