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Among the main optical methods digital image correlation (DIC) can be highlighted. Although DIC development is relatively recent, the advantages offered by this method make it equally competitive with other classical optical techniques that suffer from intrinsic limitation. The need for global field measurements is especially true in situations where the loading conditions create a heterogeneous strain field. Thus, DIC has been presented as the most adequate measurement technique for studying the behavior of polymeric materials exposed to large deformation. As a consequence of their inherent characteristics, polymers have been increasingly used in engineering applications, especially thermoplastics. Allied to its low density, which provides a considerable weight reduction and an increase of efficiency in many applications, polymers also have easy processability and good mechanical properties. Thus, a precise knowledge of polymers behavior is needed in order to improve their use capabilities. Despite DIC consistent evolution, especially in the image acquisition system, it is still an inaccessible optical measurement technique due to the high price of digital processing and image correlation software. Therefore, the present work aims to develop a low-cost digital image correlation (DIC) system to evaluate thermoplastic behavior at large deformations. In order to improve the discussion three different types of polymers were tested: high density polyethylene (PE-HD), polypropylene (PP) and polyvinyl chloride (PVC). [ABSTRACT FROM AUTHOR]

The emergence of antibiotic resistance drives an essential race against time to reveal new molecular structures capable of addressing this alarming global health problem. Snake venoms are natural catalogs of multifunctional toxins and privileged frameworks, which serve as potential templates for the inspiration of novel treatment strategies for combating antibiotic resistant bacteria. Phospholipases A2 (PLA2s) are one of the main classes of antibacterial biomolecules, with recognized therapeutic value, found in these valuable secretions. Recently, a number of biomimetic oligopeptides based on small fragments of primary structure from PLA2 toxins has emerged as a meaningful opportunity to overcome multidrug‐resistant clinical isolates. Thus, this review will highlight the biochemical and structural properties of antibacterial PLA2s and peptides thereof, as well as their possible molecular mechanisms of action and key roles in development of effective therapeutic strategies. Chemical strategies possibly useful to convert antibacterial peptides from PLA2s to efficient drugs will be equally addressed. [ABSTRACT FROM AUTHOR]

The screw withdrawal resistance and the mechanical behavior of agglomerated panels reinforced with coir and pejibaye fibers were evaluated using through and non-through screw withdrawal tests. Widespread commercially materials, such as Pinus solid wood, Medium Density Fiberboard (MDF) and plywood, were also tested in order to compare the behavior and results obtained with the lignocellulosic-reinforced agglomerated panels. Among the conventional materials tested, the highest maximum withdrawal load/thickness ratio was measured in solid wood. Panels reinforced with natural fibers showed lower withdrawal resistance than conventional materials. However, panels reinforced with coir fibers reached about 74% of the maximum strength achieved by MDF samples. Panels reinforced with pejibaye showed the worst mechanical performance. Hybridization between pejibaye and coir fibers resulted, however, in a performance improvement of approximately 50% in the maximum withdrawal load comparing with panels reinforced only with pejibaye fibers. The behavior along screw withdrawal was analyzed and could be split into four different steps. Each of these steps was correlated to the screw withdrawal rate, and also with the interactions between the material and the screw. The mechanisms involved in each step are presented and discussed. [ABSTRACT FROM AUTHOR]

Magnesium boron fibrous composites have been used in the aeronautical/aerospace due to their resistance/weight properties. The focus of the study was to collect detailed behaviour of thermal expansion for controlled rates of heating and thermal cycles through experiments. The results were compared with Schapery model and rule of mixtures for a range of temperatures and fibre orientations. The measured values of coefficient of thermal expansion values were in good agreement with the theoretical calculations for the considered 0 and 60 degrees fibre orientations. The behaviour for 90 degrees orientation was dominated by the thermal expansion of magnesium matrix as expected. [ABSTRACT FROM AUTHOR]

Despite the considerable global impact of snakebite envenoming, available treatments remain suboptimal. Here, we report the discovery of a broadly-neutralizing human monoclonal antibody, using a phage display-based cross-panning strategy, capable of reducing the cytotoxic effects of venom phospholipase A 2 s from three different snake genera from different continents. This highlights the potential of utilizing monoclonal antibodies to develop more effective, safer, and globally accessible polyvalent antivenoms that can be widely used to treat snakebite envenoming., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The membrane-active nature of phospholipase A 2 -derived peptides makes them potential candidates for antineoplastic and antibacterial therapies. Two short 13-mer C-terminal fragments taken from snake venom Lys49-PLA 2 toxins (p-AppK and p-Acl), differing by a leucine/phenylalanine substitution, were synthesized and their bioactivity was evaluated. Their capacity to interfere with the survival of Gram-positive and Gram-negative bacteria as well as with solid and liquid tumors was assessed in vitro. Toxicity to red blood cells was investigated via in silico and in vitro techniques. The mode of action was mainly studied by molecular dynamics simulations and membrane permeabilization assays. Briefly, both peptides have dual activity, i.e., they act against both bacteria, including multidrug-resistant strains and tumor cells. All tested bacteria were susceptible to both peptides, Pseudomonas aeruginosa being the most affected. RAMOS, K562, NB4, and CEM cells were the main leukemic targets of the peptides. In general, p-Acl showed more significant activity, suggesting that phenylalanine confers advantages to the antibacterial and antitumor mechanism, particularly for osteosarcoma lines (HOS and MG63). Peptide-based treatment increased the uptake of a DNA-intercalating dye by bacteria, suggesting membrane damage. Indeed, p-AppK and p-Acl did not disrupt erythrocyte membranes, in agreement with in silico predictions. The latter revealed that the peptides deform the membrane and increase its permeability by facilitating solvent penetration. This phenomenon is expected to catalyze the permeation of solutes that otherwise could not cross the hydrophobic membrane core. In conclusion, the present study highlights the role of a single amino acid substitution present in natural sequences towards the development of dual-action agents. In other words, dissecting and fine-tuning biomembrane remodeling proteins, such as snake venom phospholipase A 2 isoforms, is again demonstrated as a valuable source of therapeutic peptides.

A basic sPLA 2 (D49) from the venom of snake Agkistrodon piscivorus leucostoma (AplTX-II) was isolated, purified and characterized. We determined the enzymatic and pharmacological profiles of this toxin. AplTX-II was isolated with a high level of purity through reverse phase chromatography and molecular exclusion. The enzyme showed pI 9.48 and molecular weight of 14,003 Da. The enzymatic activity of the AplTX-II depended on Ca 2+ pH and temperature. The comparison of the primary structure with other sPLA 2 s revealed that AplTX-II presented all the structural reasons expected for a basic sPLA 2 s. Additionally, we have resolved its structure with the docked synthetic substrate NOBA (4-nitro-3-octanoyloxy benzoic acid) by homology modeling, and performed MD simulations with explicit solvent. Structural similarities were found between the enzyme's modeled structure and other snake sPLA 2 X-Ray structures, available in the PDB database. NOBA and active-site water molecules spontaneously adopted stable positions and established interactions in full agreement with the reaction mechanism, proposed for the physiological substrate, suggesting that NOBA hydrolysis is an excellent model to study phospholipid hydrolysis., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2018 Elsevier B.V. All rights reserved.)

The emergence of antibiotic resistance drives an essential race against time to reveal new molecular structures capable of addressing this alarming global health problem. Snake venoms are natural catalogs of multifunctional toxins and privileged frameworks, which serve as potential templates for the inspiration of novel treatment strategies for combating antibiotic resistant bacteria. Phospholipases A 2 (PLA 2 s) are one of the main classes of antibacterial biomolecules, with recognized therapeutic value, found in these valuable secretions. Recently, a number of biomimetic oligopeptides based on small fragments of primary structure from PLA 2 toxins has emerged as a meaningful opportunity to overcome multidrug-resistant clinical isolates. Thus, this review will highlight the biochemical and structural properties of antibacterial PLA 2 s and peptides thereof, as well as their possible molecular mechanisms of action and key roles in development of effective therapeutic strategies. Chemical strategies possibly useful to convert antibacterial peptides from PLA 2 s to efficient drugs will be equally addressed., (© 2018 Wiley Periodicals, Inc.)

Phospholipases A 2 (PLA 2 s) are important enzymes present in snake venoms and are related to a wide spectrum of pharmacological effects, however the toxic potential and therapeutic effects of acidic isoforms have not been fully explored and understood. Due to this, the present study describes the isolation and biochemical characterization of two new acidic Asp49-PLA 2 s from Bothrops brazili snake venom, named Braziliase-I and Braziliase-II. The venom was fractionated in three chromatographic steps: ion exchange, hydrophobic interaction and reversed phase. The isoelectric point (pI) of the isolated PLA 2 s was determined by two-dimensional electrophoresis, and 5.2 and 5.3 pIs for Braziliase-I and II were observed, respectively. The molecular mass was determined with values ​​of 13,894 and 13,869Da for Braziliase-I and II, respectively. Amino acid sequence by Edman degradation and mass spectrometry completed 87% and 74% of the sequences, respectively for Braziliase-I and II. Molecular modeling of isolated PLA 2 s using acid PLA 2 BthA-I-PLA 2 from B. jararacussu template showed high quality. Both acidic PLA 2 s showed no significant myotoxic activity, however they induced significant oedematogenic activity. Braziliase-I and II (100μg/mL) showed 31.5% and 33.2% of cytotoxicity on Trypanosoma cruzi and 26.2% and 19.2% on Leishmania infantum, respectively. Braziliase-I and II (10μg) inhibited 96.98% and 87.98% of platelet aggregation induced by ADP and 66.94% and 49% induced by collagen, respectively. The acidic PLA 2 s biochemical and structural characterization can lead to a better understanding of its pharmacological effects and functional roles in snakebites pathophysiology, as well as its possible biotechnological applications as research probes and drug leads., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Epidermal growth factor can activate several signaling pathways, leading to proliferation, differentiation, and tumorigenesis of epithelial tissues by binding with its receptor. The EGF protein is involved in nervous system development, and polymorphisms in the EGF gene on chromosome band 4q25 are associated with brain cancers. The purpose of this study was to investigate the association between the single-nucleotide polymorphism of EGF+61G/A and extraaxial brain tumors in a population of the southeast of Brazil. We analyzed the genotype distribution of this polymorphism in 90 patients and 100 healthy subjects, using the polymerase chain reaction-restriction fragment length polymorphism technique. Comparison of genotype distribution revealed a significant difference between patients and control subjects (P < 0.001). The variant genotypes of A/G and G/G were associated with a significant increase of the risk of tumor development, compared with the homozygote A/A (P < 0.0001). When the analyses were stratified, we observed that the genotype G/G was more frequent in female patients (P=0.021). The same genotype was observed more frequently in patients with low-grade tumors (P=0.001). Overall survival rates did not show statistically significant differences. Our data suggest that the EGF A61G polymorphism can be associated with susceptibility to development of these tumors., (Copyright 2010 Elsevier Inc. All rights reserved.)