551. RNA trafficking in oligodendrocytes.
- Author
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Carson JH, Cui H, Krueger W, Schlepchenko B, Brumwell C, and Barbarese E
- Subjects
- Active Transport, Cell Nucleus, Animals, Cell Nucleus metabolism, Computer Simulation, Cytoplasmic Granules genetics, Cytoplasmic Granules metabolism, Heterogeneous-Nuclear Ribonucleoproteins, Microtubules drug effects, Microtubules metabolism, Models, Biological, Molecular Motor Proteins metabolism, Myelin Basic Protein genetics, Paclitaxel pharmacology, Protein Biosynthesis, Protein Transport, RNA genetics, Ribonucleoproteins genetics, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Myelin Basic Protein metabolism, Oligodendroglia metabolism, RNA metabolism, Ribonucleoproteins metabolism
- Abstract
A2RE and hnRNP A2 have been identified as important cis/trans determinants for MBP RNA trafficking in oligodendrocytes. Since A2RE-like sequences are found in several different transported RNAs, and since hnRNP A2 is expressed in most cell types, this may represent a general RNA trafficking pathway shared by a variety of different RNAs in different cell types. In oligodendrocytes, A2RE/hnRNP A2 determinants are involved in at least four steps in the RNA trafficking pathway: (1) export from the nucleus to the cytoplasm, (2) granule assembly in the perikaryon, (3) transport along microtubules in the processes, and (4) translation activation in the myelin compartment. The components of the cellular machinery mediating each of these steps are known. How A2RE/hnRNP A2 determinants interact with these components to mediate RNA trafficking is being investigated by a combination of: biochemistry to analyze molecular interactions in vitro, imaging to visualize molecular interactions in living cells, and computational modeling to simulate molecular interactions in the Virtual Cell.
- Published
- 2001
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