501. Glycosidase activities in the rat liver microsomes
- Author
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Zensaku Yosizawa, Mamoru Isemura, Teruo Ono, and Hiroko Kosaka
- Subjects
Glycoside Hydrolases ,Submitochondrial Particles ,General Biochemistry, Genetics and Molecular Biology ,Rat liver microsomes ,alpha-Mannosidase ,Lysosome ,Acetylglucosaminidase ,Mannosidases ,medicine ,Animals ,Glycoside hydrolase ,chemistry.chemical_classification ,Chromatography ,biology ,Elution ,beta-Glucosidase ,Rats, Inbred Strains ,General Medicine ,beta-Galactosidase ,Rats ,Enzyme ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Concanavalin A ,Rat liver ,biology.protein ,Microsome ,Microsomes, Liver - Abstract
KOSAKA, H., ISEMURA, M., YOSIZAWA, Z., and ONO, T., Glycosidase Activities in the Rat Liver Microsomes. Tohoku J. exp. Med., 1985, 146 (2), 201-210-The glycosidase activities in a rat liver microsome fraction were investigated in comparison with those in a submitochondrial fraction. The activities of β-N-acetylglucosaminidase, β-galactosidase and β-glycosidase in the former were distinguished from those in the latter on the basis of solubilization pattern, pH optimum, thermal stability and elution pattern of concanavalin A-Sepharose chromatography. It is suggested that these activities are not due to the contamination of lysosomal enzymes but intrinsic to microsomes.
- Published
- 1985