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Wild lagomorphs including rabbits and hares can act as natural carriers or reservoirs of bacterial and parasitic zoonotic diseases. However, little is known on the epidemiology and potential public health significance of intestinal eukaryotes in wild leporids. We examined faecal samples from European wild rabbits (Oryctolagus cuniculus, n = 438) and Iberian hares (Lepus granatensis, n = 111) collected in the Autonomous Region of Andalusia in southern Spain during 2012–2021. We searched for the presence of DNA from the main intestinal protist and microsporidial pathogens of veterinary and public health concerns using molecular methods (PCR followed by Sanger and next‐generation sequencing). Giardia duodenalis was the most prevalent species found (27.8%, 153/550; 95% CI: 24.1–31.8), followed by Cryptosporidium spp. (1.3%, 7/550; 95% CI: 0.5–2.6), Blastocystis sp. (1.1%, 6/550; 95% CI: 0.4–2.4) and Encephalitozoon intestinalis (0.2%, 1/550; 95% CI: 0.0–10.1). All samples tested negative for Enterocytozoon bieneusi. Sequence analyses revealed the presence of sub‐assemblage BIV (n = 1) within G. duodenalis, and Cryptosporidium cuniculus (n = 6) and Cryptosporidium andersoni (n = 1) within Cryptosporidium. The presence of ruminant‐adapted C. andersoni is indicative of a potential cross‐species transmission event, although a spurious infection (mechanical carriage) cannot be ruled out. Samples assigned to C. cuniculus belonged to the gp60 subtype families Va (n = 3) and Vb (n = 2). The six Blastocystis‐positive samples were identified as ST2 (n = 3) and ST1 + ST2 (n = 3). Our molecular results suggest that wild rabbits and hares were primarily infected by leporid‐adapted species of eukaryotic pathogens. However, the occasional findings of zoonotic G. duodenalis sub‐assemblage BIV, Blastocystis sp. ST1 and ST2, and Encephalitozoon intestinalis could be of public health relevance. [ABSTRACT FROM AUTHOR]

Apart from humans, other predators can take part in creating bone accumulations by generating waste materials that may have been mixed with those produced by hominids and leading to the formation of palimpsests. This is discussed here in the case of Middle Paleolithic leporid assemblages, to which carnivores have frequently contributed. Level III of Teixoneres Cave (MIS 3) is a sample that can be used to address the origin of leporid assemblages in archaeological contexts. Applying an archaeozoological and taphonomical methodology has made it possible to state that the assemblage of leporids in the site has been generated by a mix of contributions, in which small mammal carnivores and nocturnal raptors seem to play an important role, together with occasional hominid inputs. The aim of this paper is to present new data about Neanderthal activities at this site and support the hypothesis related to short-term human occupations in the cave. [ABSTRACT FROM AUTHOR]

Abstract: A new species of leporid (Lagomorpha, Mammalia) is described based on the material from the Lower Pleistocene of Cueva Victoria (Murcia, Spain). This species, named Oryctolagus giberti n. sp., presents several intermediate characters between the first known representative of the genus, O. laynensis, from the Middle Pliocene of Spain, and the modern European rabbit (O. cuniculus), specially at the level of the palate, the mandible, the third lower premolar, the ulna, the coxal and the femur. In this respect, Oryctolagus giberti n. sp. is a firm candidate to occupy the vacant phylogenetic place between these two species. It also questions the results of molecular studies that date the divergence of groups A and B of Oryctolagus cuniculus at about 2Myr ago. [Copyright &y& Elsevier]

The article discusses a study on the captive breeding of wild rabbits. The main purpose of the study is to design a semi-extensive system for breeding wild rabbits in captivity that offers an efficient way to maintain the genetic diversity of rabbits. The rabbits used for the study originated from San Silvestre de Guzman in Southern Spain. Key findings of study indicate that the use of artificial burrows contribute to resolve most of the management problems associated with wild rabbit breeding in captivity.

Leporid (rabbit and hare) bones have been shown to yield important information about subsistence practices, mobility patterns, and demographic trends during the Paleolithic of the western and eastern Mediterranean regions. Studies of Spanish Paleolithic caves rich in rabbit bones suggest that residential mobility patterns influence the degree of leporid hunting through time. Studies of Paleolithic sites in the eastern Mediterranean suggest that leporids were hunted in large numbers only after population sizes and densities reached certain thresholds. This paper reviews and critiques these studies based on current taphonomic and ecologic information about leporids. Leporid hunting during the Upper Paleolithic of central Portugal is then discussed and compared to these existing models. These latter data suggest that rabbit hunting in central Portugal does not conform to any existing model, suggesting that local factors of leporid density and environmental conditions likely influenced the nature and timing of small game acquisition during the Upper Paleolithic. [ABSTRACT FROM AUTHOR]

Viruses are ubiquitous in nature; however, very few have been identified in the Leporid species. In the fall of 2018, an outbreak of myxomatosis in Iberian hares (Lepus granatensis) was reported in Spain and a novel recombinant myxoma virus strain (MYXV-Tol) was identified. To investigate variability within the recombinant region of the MYXV-Tol and identify any potential viral coinfections, samples (ear, eyelid or vaginal) of Iberian hares were collected from Spain and analyzed. The presence of the recombinant region of the MYXV-Tol was confirmed in six out of eleven samples analyzed. Additionally, a polyomavirus (family Polyomaviridae), representing a putative new species, and anelloviruses (family Anelloviridae) belonging to two putative species were identified, some as coinfection with the recombinant MYXV-Tol. The two polyomavirus genomes were identified in two hares and share >99% genome-wide identity. Based on the analysis of their large T-antigen, the new polyomavirus clusters in a distant clade from other mammals sharing <64% amino acid identity. A total of 14 anelloviruses were identified, which share 63–99% genome-wide identity. Overall, our results show a coinfection of different DNA viruses in the studied samples and raise awareness regarding the extensive unsampled diversity of viruses in hares. [ABSTRACT FROM AUTHOR]