1. Carbapenemase-producing Enterobacteriaceae: a 2-year surveillance in a hospital in Iaşi, Romania.
- Author
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Braun SD, Dorneanu OS, Vremeră T, Reißig A, Monecke S, and Ehricht R
- Subjects
- Aged, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Bacterial Proteins genetics, Drug Resistance, Multiple, Bacterial, Enterobacter cloacae drug effects, Enterobacter cloacae genetics, Enterobacteriaceae drug effects, Enterobacteriaceae genetics, Enterobacteriaceae isolation & purification, Enterobacteriaceae Infections mortality, Female, Genotype, Humans, Klebsiella pneumoniae drug effects, Klebsiella pneumoniae genetics, Male, Microbial Sensitivity Tests, Middle Aged, Phenotype, Prevalence, Romania epidemiology, beta-Lactamases genetics, Bacterial Proteins biosynthesis, Enterobacteriaceae enzymology, Enterobacteriaceae Infections epidemiology, Enterobacteriaceae Infections microbiology, Epidemiological Monitoring, Hospitals statistics & numerical data, beta-Lactamases biosynthesis
- Abstract
Aim: Limited information is currently available about the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in Romania., Materials & Methods: Routine tests of 1,993 clinical isolates at a hospital in Iaşi yielded 46 isolates that were resistant to carbapenems. All 46 isolates were phenotypically and genotypically analyzed using VITEK-2 and DNA microarray-based assays., Results: Isolates were assigned to Klebsiella pneumoniae and Enterobacter cloacae. For 39 isolates, carbapenem resistance was confirmed and 37 harbored at least one carbapenem resistance gene. Two isolates were probably resistant due to AmpC β-lactamases in combination with a porin loss. The overall concordance between detected phenotype and genotype was 95%., Conclusion: Our data show that carbapenemase-producing isolates with different underlying resistance mechanisms are still rare in Iaşi, but the global rise of CPE warrants intensified surveillance.
- Published
- 2016
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