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Carbapenemase-producing Enterobacteriaceae: a 2-year surveillance in a hospital in Iaşi, Romania.
- Source :
-
Future microbiology [Future Microbiol] 2016; Vol. 11 (3), pp. 391-401. Date of Electronic Publication: 2016 Mar 14. - Publication Year :
- 2016
-
Abstract
- Aim: Limited information is currently available about the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in Romania.<br />Materials & Methods: Routine tests of 1,993 clinical isolates at a hospital in Iaşi yielded 46 isolates that were resistant to carbapenems. All 46 isolates were phenotypically and genotypically analyzed using VITEK-2 and DNA microarray-based assays.<br />Results: Isolates were assigned to Klebsiella pneumoniae and Enterobacter cloacae. For 39 isolates, carbapenem resistance was confirmed and 37 harbored at least one carbapenem resistance gene. Two isolates were probably resistant due to AmpC β-lactamases in combination with a porin loss. The overall concordance between detected phenotype and genotype was 95%.<br />Conclusion: Our data show that carbapenemase-producing isolates with different underlying resistance mechanisms are still rare in Iaşi, but the global rise of CPE warrants intensified surveillance.
- Subjects :
- Aged
Anti-Bacterial Agents pharmacology
Anti-Bacterial Agents therapeutic use
Bacterial Proteins genetics
Drug Resistance, Multiple, Bacterial
Enterobacter cloacae drug effects
Enterobacter cloacae genetics
Enterobacteriaceae drug effects
Enterobacteriaceae genetics
Enterobacteriaceae isolation & purification
Enterobacteriaceae Infections mortality
Female
Genotype
Humans
Klebsiella pneumoniae drug effects
Klebsiella pneumoniae genetics
Male
Microbial Sensitivity Tests
Middle Aged
Phenotype
Prevalence
Romania epidemiology
beta-Lactamases genetics
Bacterial Proteins biosynthesis
Enterobacteriaceae enzymology
Enterobacteriaceae Infections epidemiology
Enterobacteriaceae Infections microbiology
Epidemiological Monitoring
Hospitals statistics & numerical data
beta-Lactamases biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1746-0921
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Future microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26974389
- Full Text :
- https://doi.org/10.2217/fmb.15.148