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The Gram-stain-negative, orange-pigmented, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated TP-CH-4 T , was isolated from a seamount near the Yap Trench in the tropical western Pacific. The optimal growth conditions were determined to be at pH 7-8, 25-30 °C and in the presence of 2 % (w/v) NaCl. The major respiratory quinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids and three unidentified polar lipids. The predominant cellular fatty acids were iso-C 15 : 0 and summed feature 1 (composed of C 13 : 0 3-OH and/or iso-C 15 : 1 H). Phylogenetic analysis of 16S rRNA gene sequences revealed that strain TP-CH-4 T was a member of the family Flavobacteriaceae and formed a distinct lineage. Strain TP-CH-4 T displayed highest sequence similarities to Pseudozobellia thermophila KMM 3531 T (95.1 %) and Flagellimonas flava A11 T (93.9 %). Genome sequencing revealed the strain TP-CH-4 T has a genome size of 4.5 Mbp and a G+C content of 44.5 mol%. Collectively, based on phenotypic, chemotaxonomic, phylogenetic and genomic evidence, strain TP-CH-4 T represents a novel species of a novel genus of the family Flavobacteriaceae , for which the name Pelagihabitans pacificus gen. nov., sp. nov. is proposed. The type strain of Pelagihabitans pacificus is TP-CH-4 T (=CGMCC 1.17120 T =KCTC 72434 T ).

The Gram-stain-negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP390 T , was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain TP390 T was related to the genus Flavobacterium and had highest 16S rRNA gene sequence identity with the type strain of Flavobacterium jejuense EC11 T (97.8 %). Sequence similarities to all other type strains of current species of the genus Flavobacterium were below 97 %. The predominant cellular fatty acids were iso-C 15 : 0 and iso-C 15 : 1 G. The quinone system for strain TP390 T comprised predominantly menaquinone MK-6 and the polar lipid profile contained phosphatidylethanolamine, four unknown aminolipids, one glycolipid and six unknown polar lipids. The genomic DNA G+C content of strain TP390 T was 31.2 mol%. In addition, the maximum values of in silico DNA-DNA hybridization ( is DDH) and average nucleotide identity (ANI) between strain TP390 T with F. jejuense KCTC 42149 T were 22.60 and 80.01% respectively. Combined data from phenotypic, phylogenetic, is DDH and ANI data demonstrated that the strain TP390 T is representative of a novel species of the genus Flavobacterium , for which we propose the name Flavobacterium profundi sp. nov. (type strain TP390 T =KACC 18559 T =CGMCC 1.15398 T ).

The Gram-stain-negative, rod-shaped, yellow-pigmented and facultative anaerobic bacterial strain, designated H164 T , was isolated from seawater collected from the Caroline Seamounts in the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H164 T was related to the genus Algibacter and had highest 16S rRNA gene sequence similarity to Algibacter wandonensis WS-MY22 T (97.4 %). The major cellular fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 15 : 1 G, iso-C 15 : 0 3-OH and iso-C 17 : 0 3-OH. The predominant menaquinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The genomic DNA G+C content of strain H164 T was 33.2 mol%. The values of in silico DNA-DNA hybridization ( is DDH) and average nucleotide identity (ANI) between strain H164 T and A. wandonensis KCTC 32381 T were 26.10 and 81.88 %. The is DDH and ANI values between strain H164 T and Algibacter lectus DSM 15365 T were 25.40 and 81.79 %. Combined data from phenotypic, phylogenetic, is DDH and ANI analyses demonstrated that strain H164 T represents a novel species of the genus Algibacter , for which we propose the name Algibacter pacificus sp. nov. (type strain H164 T =KCTC 72432 T =CGMCC 1.17117 T ).

A Gram-stain negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP187 T , was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain TP187 T is related to members of the genus Vibrio and has high 16S rRNA gene sequence similarity with the type strains of Vibrio chagasii (97.3%) and Vibrio gallaecicus (97.1%). Sequence similarities to all other type strains of current species of the genus Vibrio were below 97%. The polar lipids profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, an aminophospholipid, two aminolipids, four phospholipids and eleven unidentified polar lipids. Ubiquinone Q-8 was detected as the predominant quinone. The genomic DNA G + C content of strain TP187 T was determined to be 43.7 mol%. In addition, the maximum values of in silico DNA-DNA hybridization (isDDH) and average nucleotide identity (ANI) between strain TP187 T with V. chagasii LMG 21353 T were 22.40 and 77.50% respectively. Both values are below the proposed cutoff levels for species delineation, i.e. 70 and 95%, respectively. Combined data from phenotypic, phylogenetic, isDDH and ANI data demonstrated that the strain TP187 T is representative of a novel species of the genus Vibrio, for which we propose the name Vibrio profundi sp. nov. (type strain TP187 T  = KACC 18555 T  = CGMCC 1.15395 T ).