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Background: The Pediatric Eating Assessment Tool (PEDI-EAT-10) is a reliable and valid tool for rapid identification of dysphagia in patients aged 18 months to 18 years. Aims: To translate and adapt the PEDI-EAT-10 into the Italian language and evaluate its validity and reliability. Methods & Procedures: The translation and cross-cultural adaptation of the tool consisted of five stages: initial translation, synthesis of the translations, back translation, expert committee evaluation and test of the prefinal version. The internal consistency of the translated tool was analysed in a clinical group composed of 200 patients with special healthcare needs aged between 18 months and 18 years. They were consecutively enrolled at the Rare Disease Unit, Paediatrics Department, Fondazione Policlinico Agostino Gemelli-IRCCS, Rome. For test-retest reliability, 50 caregivers filled in the PEDI-EAT-10 questionnaire for a second time after a 2-week period. Construct validity was established by comparing data obtained from patients with data from healthy participants (n = 200). The study was approved by the local ethics committee. Outcomes & Results: Psychometric data obtained from patients (104 M; mean age = 8.08 ± 4.85 years; median age = 7 years) showed satisfactory internal consistency (Cronbach's a = 0.89) and test-retest reliability (Pearson r = 0.99; Spearman r = 0.96). A total of 30% of children were classified as having a high risk of penetration/aspiration. The Italian PEDI-EAT-10 mean total score of the clinical group was significantly different from that resulting from healthy participants. Conclusions & Implications: The PEDI-EAT-10 was successfully translated into Italian, validated and found to be a reliable one-page rapid screening tool to identify dysphagia in children and adolescents with special needs.

Background: The Montreal Children's Hospital Feeding Scale (MCH-FS) allows paediatricians and other health care professionals to identify feeding difficulties among children. Aim: To translate and adapt the MCH-FS into Italian, and to evaluate the validity and reliability of this Italian version of the Montreal Children's Hospital Feeding Scale (I-MCH-FS). Methods & Procedures: A total of 150 children with special healthcare needs were admitted to the Rare Disease Unit of the Paediatrics Department at the Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy, between March 2021 and March 2022 (74 males; mean age = 3.85 ± 1.96 years; median age = 4 years; age range = 6 months-6 years and 11 months) and 150 healthy participants (83 males; mean age = 3.5 ± 1.98 years; median age = 3 years; age range = 6 months-6 years and 11 months) were included in the study, which was approved by the local ethics committee. The original version of the MCH-FS was translated and cross-cultural adapted through five stages: (1) initial translation, (2) synthesis of the translations, (3) back translation, (4) expert committee and (5) test of the prefinal version. Test-retest reliability and internal consistency were assessed using Pearson r, Spearman r and Cronbach's alpha, respectively. Construct validity was established by comparing data obtained from patients with those of healthy participants using the Mann-Whitney U-test. Outcomes & Results: A Pearson "r" of 0.98, a Spearman "r" of 0.95 and Cronbach's alpha value of 0.86 were obtained. In the clinical group, 40.6% children were classified as having feeding disorders (n = 61), while in the normative group 4.7% were diagnosed with feeding problems (n = 7). Mean total score of the clinical group was significatively different from the normative's. Conclusions & Implications: The I-MCH-FS is a valid and reliable one-page, quick screening tool used to identify feeding disorders among children with special needs in outpatient paediatric setting.

Actions to counter violence against women are a fully fledged policy field with significant differences across countries. Through a comparative analysis of Spain and Italy, this article maps the interplay between women's movements and national governments in launching violence against women (VAW) policies. In Spain, policy formation was the outcome of dual feminist–socialist activism, leading to dialogue between movements and the government. In Italy, movements opposed the government from the outside. In both countries, the critical factor inciting responsiveness on VAW was not one single variable but a combination of political opportunity, movement identity, dedicated women's policy agencies, and the soft power of international institutions. [ABSTRACT FROM AUTHOR]

Although studies have highlighted the role played by couple and parental relationships for children's psychosocial adjustment, especially in challenging situations, research on these two relationship domains has largely developed separately and mostly focussed on negative couple processes. However, Family Systems Theory highlights how these subsystems are interconnected, and studies inspired by this theoretical framework provide evidence of how the quality of the interparental relationship predicts the parent–child relationship. This study focussed on the association between two relational resources (dyadic coping and parent–child relationship quality) and children's emotional difficulties during the outbreak of the COVID‐19 pandemic in Italy. Five hundred ninety‐one Italian parents filled in an online self‐report questionnaire. A path analysis model was estimated to test the mediating role of parent–child relationship quality in the association between partner positive dyadic coping and children's emotional difficulties. The findings showed both a direct effect and an indirect effect of partner positive dyadic coping on children's emotional difficulties, because parent–child relationship quality partially mediated this association. Enhancing parents' ability to cope together with stress and the quality of the parent–child relationship might contribute to decrease children's vulnerability to emotional difficulties in challenging times. [ABSTRACT FROM AUTHOR]

Comparative political economy scholarship struggles to categorise Italy's model of capitalism between a mixed-market economy and a hybrid, stagnant economic system. To enhance our understanding of the Italian political economy, this article employs the analytical framework of growth regimes to study Italy's regional economic systems. Our analysis indicates that Italy can hardly be defined as a 'national growth regime' due to the presence of two diametrically opposed regional growth regimes: Northern regions conform to a manufacturing-based, export-led growth regime supported by competitiveness-enhancing territorial institutions; southern regions conform to a particular variety of the consumption-led growth regime, that is, an administrative Keynesianism regime, which we theorise to typify a regime where growth and employment are systematically dependent on the state's role of employer of last resort, the state's consumption-enhancing social policies and economic forbearance of labour and corporate tax regulations. The article suggests that studying regional growth regimes is desirable when marked internal diversity in economic outcomes or productive structures exists across regions within (generally larger) countries, and when subnational governments have powers to develop major own institutions/policies in support of regional growth regimes. [ABSTRACT FROM AUTHOR]

Background: Rare diseases constitute a heterogeneous group of approximately 7000–8000 conditions, distinguished by their low prevalence. Collectively, they present a significant global health challenge, affecting millions of people worldwide. It is estimated that rare diseases affect approximately 10% of the global population, which places a significant burden on individuals, families, and society. It is, therefore, important to consider strategies to improve the overall well-being and quality of life of individuals with rare diseases. One potential avenue for exploration is the incorporation of physical activity (PA). The scope of this study was to ascertain whether PA has a positive impact on measures of well-being and to determine its potential to enhance the quality of life of these individuals. Methods: The data were collected via an online survey. The one-way ANOVA test for multiple groups and multivariate Poisson models were employed to identify the significant predictors of the outcomes of interest. Results: The protective effects of PA become evident with a minimum of six hours of activity per week. Our data confirm that the weekly hours devoted to PA can serve as a significant protective factor for QoL. The study also provided some insights into the motivations behind patients' engagement in PA. These included improving QoL and physical well-being, as well as the desire to interact socially, with the goal of meeting friends or making new acquaintances. Finally, for adults and older adults, engaging in PA can also be a way to control weight. Conclusions: It is becoming increasingly clear that individuals with rare diseases stand to benefit greatly from PA, so it is only sensible to educate them on the advantages of an active lifestyle. [ABSTRACT FROM AUTHOR]

The monitoring of stranded marine mammals represents a strategic method to assess their health, conservation status, and ecological role in the marine ecosystem. Networks worldwide track stranding events for the passive monitoring of mortality patterns, emerging and reemerging pathogens, climate change, and environmental degradation from a One Health perspective. This study summarizes pathogen prevalence data from the Italian Stranding Network (ISN) derived from post-mortem investigations on cetaceans found dead stranded along the Italian coastline between 2015 and 2020. The decomposition of the carcasses and logistics limited the post-mortem examination to 585 individuals, out of 1236 single-stranding reports. The most relevant pathogens identified were Cetacean Morbillivirus, Herpesvirus, Brucella spp., and Toxoplasma gondii, whose roles as environmental stressors are well known, despite their real impact still needing to be investigated in depth. Statistical analysis showed that age and sex seem to be positively related to the presence of pathogens. This study represents the first step in harmonizing post-mortem investigations, which is crucial for evidence-based conservation efforts. Implementing diagnostic and forensic frameworks could offer an indirect insight into the systematic monitoring of diseases to improve the identification of regional and temporal hotspots in which to target specific mitigation, management, and conservation strategies. [ABSTRACT FROM AUTHOR]

Academics have always been endowed with the privilege of autonomy, but the diffusion of evaluation systems based on publication outcomes potentially jeopardizes the benefits deriving from behaviors that address other pillars of higher education. Besides research and teaching, academic citizenship, i.e., the service behaviors carried out within and outside organizational boundaries, are in fact cornerstones of university functioning. We investigate the relationship between academic citizenship and research after the introduction of an evaluation system that moves research performance to center stage on a dataset collecting publication records and service activities of 353 Italian scholars in the accounting discipline in the 2004-2013 period. A cluster analysis reveals different academics' orientations towards research and academic citizenship. We contribute to the debate on academic choices by showing that a large number of university members tend to focus on a single type of academic citizenship or to adopt a research orientation, while a significant part remains stuck in the middle without achieving satisfying performance in any domains according to international standards, and discuss implications for the design of behavioral incentives.

Research excellence has been the main thrust of higher education reforms in several countries, but its translation into coherent policies has proved to be controversial. Literature has delved into the role exerted by contextual factors and individual characteristics in the adoption of behaviours inspired by social values. Our study aims at investigating the effect of individual and contextual features and their interplay in the quest for research excellence. We formulate and test hypotheses on a dataset collecting the publication records of the population of 4510 Italian academics in business and management and in architecture in the 2004-2013 period. Findings disclose that, alongside previous achievements, research-oriented settings favour research excellence. In addition, we show that a research-oriented context enhances the publication record of academics without highly ranked publications, whereas it slightly reduces the productivity of outstanding academics. Implications for public policy, especially in terms of performance measurement systems, are presented.

This study evaluates the nutritional quality and level of processing of food and beverage products advertised on television channels most watched by children, in Italy. Data were collected over 20 non-consecutive days from the five top channels in Italy. A total of 209 food items were analysed using the Nutri-Score and the NOVA classification. Additionally, compliance with nutritional criteria established by the World Health Organisation (WHO) and the EU-PLEDGE was assessed. Results indicate a high proportion of energy-dense and low nutritional quality foods, with 68% falling into less favourable Nutri-Score categories (C, D, and E), and 61% classified as ultra-processed by NOVA classification. When applying WHO and PLEDGE criteria, 71% and 56% of products, respectively, did not meet nutritional standards. This study underscores the low prevalence of nutritionally adequate products in food advertisements targeting children on Italian television, despite differences found based on methodological variations. [ABSTRACT FROM AUTHOR]

Introduction: Porcine circovirus 2 (PCV-2) is a key pathogen for the swine industry at a global level. Nine genotypes, differing in epidemiology and potentially virulence, emerged over time, with PCV-2a, -2b, and -2d being the most widespread and clinically relevant. Conversely, the distribution of minor genotypes appears geographically and temporally restricted, suggesting lower virulence and different epidemiological drivers. In 2022, PCV-2e, the most genetically and phenotypically divergent genotype, was identified in multiple rural farms in North-eastern Italy. Since rural pigs often have access to outdoor environment, the introduction from wild boars was investigated. Methods: Through a molecular and spatial approach, this study investigated the epidemiology and genetic diversity of PCV-2 in 122 wild boars across different provinces of North-eastern Italy. Results: Molecular analysis revealed a high PCV-2 frequency (81.1%, 99/122), and classified the majority of strains as PCV-2d (96.3%, 78/81), with sporadic occurrences of PCV-2a (1.2%, 1/81) and PCV-2b (2.5%, 2/81) genotypes. A viral flow directed primarily from domestic pigs to wild boars was estimated by phylogenetic and phylodynamic analyses. Discussion: These findings attested that the genotype replacement so far described only in the Italian domestic swine sector occurred also in wild boars. and suggested that the current heterogeneity of PCV-2d strains in Italian wild boars likely depends more on different introduction events from the domestic population rather than the presence of independent evolutionary pressures. While this might suggest PCV-2 circulation in wild boars having a marginal impact in the industrial sector, the sharing of PCV-2d strains across distinct wild populations, in absence of a consistent geographical pattern, suggests a complex interplay between domestic and wild pig populations, emphasizing the importance of improved biosecurity measures to mitigate the risk of pathogen transmission. [ABSTRACT FROM AUTHOR]

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Due to particular vulnerabilities and environmental constraints, Alpine faunas are exposed to significant threats from climate change. However, baseline diversity and distribution data to monitor the trends of key arthropod groups are often scarce. Ants are highly diversified and key ecological actors across terrestrial ecosystems, including mountain ranges. We investigated ant diversity and distribution in the Southern European Alps to provide detailed data over wide elevational gradients and make a first assessment of potential vulnerabilities in the face of global warming. We detected 40 species from 700 to over 2600 m asl, with progressively less diversity corresponding to higher elevations and lower temperatures. Maximum temperature was weakly related to ant diversity as compared to mean and minimum temperature. In shaping ant diversity, the highest elevation species had wide elevation ranges, consistent with Rapoport's rule. We documented a fauna characterized by cold-adapted genera, species with wide geographic distributions and presumably high dispersal capabilities, no dietary specializations, and a high frequency of social parasitism. Concerning Bergmann's rule, average ant species size was not larger in the coldest environments. Red wood ants, characterized by much more populous colonies as compared to the other ant species, had the highest number of ant individuals to be found at intermediate elevations and in woodland habitats. On the other hand, grasslands and shrublands proved to be the most species-rich habitats. Our data lay the groundwork for further investigation on elevational shifts and provide context for the discussion of key aspects of the management and conservation of European alpine ants. Implications for insect conservation: The cold climate of the European Alps has so far protected its ant fauna from the incursion of alien competitors as compared to what is observed in the Mediterranean region. The scarcity of species with restricted distribution ranges or high-elevation specialists, as well as diet specialists, may grant ants of the European Alps more resilience to climate change as compared to other Alpine arthropod groups. On the other hand, many species are highly interconnected by social parasitism relationships that may be vulnerable to ecological cascade effects. While forest habitats host red wood ants, which are often protected for their important ecological role, the importance of grassland must not be overlooked as they support the highest ant species richness. [ABSTRACT FROM AUTHOR]

This paper describes the aerosol in-situ laboratory at CIAO (CNR IMAA Atmospheric Observatory) in South Italy, outlining its configuration and detailing each instrument and sampling lines. The CIAO observatory has been collecting observations of atmospheric components since 2000. Initially the activities revolved around aerosol lidar, later radiosounding and cloud remote sensing observations were added over the years and made CIAO a leading atmospheric observatory in the Mediterranean region. In 2018, a significant upgrade started for enhancing the observational capability by adding aerosol in-situ instruments, with the objective to push new research boundaries for aerosol characterization and multi-instrumental synergistic approaches. Here, we describe each technical implementation step for building up an extensive aerosol in-situ laboratory compliant with ACTRIS (Aerosol Clouds and Trace gases Research InfraStructure) standard operating procedures. Starting from scratch, the long path initiated in 2018, with the design of the laboratory in terms of instruments, container organization, inlets and sampling lines optimizations, that required time and interactions with experts in the field. Reporting here all the details about the final solutions implemented at CIAO, this paper will be, for new aerosol in-situ laboratory, a practical guide for the implementation of the aerosol in-situ observational site. [ABSTRACT FROM AUTHOR]

Canine coronavirus (CCoV) is a common agent of gastroenteritis in dogs, although some variants have been found associated with systemic and often fatal diseases. Distinct genotypes (CCoV-I and CCoV-II) and subgenotypes (CCoV-IIa and CCoV-IIb) are worldwide distributed. In Italy, CCoV infections have been occasionally evaluated, but information about the molecular epidemiology and the genomic features of currently circulating strains is limited. This study reports the detection and molecular characterization of CCoV strains from samples collected from 284 dogs in Italy between 2019 and 2021. CCoV RNA was detected in 39 (13.7%) dogs, as a single viral agent (5 animals, 12.8%) or with other viral pathogens (canine parvovirus types 2a/2b/2c; canine adenovirus type 1; norovirus GIV.2) (34 animals, 87.2%). A total of 48 CCoV strains were detected either alone (CCoV-I: 51.3%, CCoV-IIa: 20.5%) or in copresence (CCoV-I and CCoV-IIa, 23.1%); surprisingly, CCoV-IIb was not identified in this study. Five clusters of CCoV-I were detected, and their spike gene sequences showed the highest nucleotide identities with CCoV-I strains collected from Greece in 2008/2009 and from China in 2021. CCoV-IIa spike gene sequences (three variants) had the highest nucleotide identities with CCoV-IIa strains collected in Greece in 2008/2009 and in Italy in 2009/2011. Given the high CCoV diversity and the variable pathogenicity potential, we underline the need of further surveillance studies to increase our understanding of the epidemiology and evolution of these viruses. [ABSTRACT FROM AUTHOR]

Background: Anopheles sacharovi, a member of the Anopheles maculipennis complex, was a historical malaria vector in Italy, no longer found since the last report at the end of 1960s. In September 2022, within the Surveillance Project for the residual anophelism, a single specimen of An. maculipennis sensu lato collected in Lecce municipality (Apulia region) was molecularly identified as An. sacharovi. This record led to implement a targeted entomological survey in September 2023. Methods: Investigation was conducted in the areas around the first discovery, focusing on animal farms, riding stables and potential breeding sites. Adult and immature mosquitoes were collected, using active search or traps, in several natural and rural sites. Mosquitoes belonging to An. maculipennis complex were identified morphologically and molecularly by a home-made routine quantitative polymerase chain reaction (qPCR) assay, developed specifically for the rapid identification of An. labranchiae, and, when necessary, by amplification and sequencing of the ITS-2 molecular marker. Results: Out of the 11 sites investigated, 6 were positive for Anopheles presence. All 20 An. maculipennis s.l. (7 adults, 10 larvae and 3 pupae) collected in the areas were identified as An. sacharovi by ITS-2 sequencing. Conclusions: The discovery of An. sacharovi, considered to have disappeared from Italy for over 50 years, has a strong health relevance and impact, highlighting an increase in the receptivity of the southern areas. As imported malaria cases in European countries are reported every year, the risk of Plasmodium introduction by gametocyte carriers among travellers from endemic countries should be taken into greater consideration. Our findings allow rethinking and building new models for the prediction and expansion of introduced malaria. Furthermore, to prevent the risk of reintroduction of the disease, the need to strengthen the surveillance of residual anophelism throughout the South should be considered. [ABSTRACT FROM AUTHOR]

Respiratory malignancies, encompassing cancers affecting the lungs, the trachea, and the bronchi, pose a significant and dynamic public health challenge. Given that air pollution stands as a significant contributor to the onset of these ailments, discerning the most detrimental agents becomes imperative for crafting policies aimed at mitigating exposure. This study advocates for the utilization of explainable artificial intelligence (XAI) methodologies, leveraging remote sensing data, to ascertain the primary influencers on the prediction of standard mortality rates (SMRs) attributable to respiratory cancer across Italian provinces, utilizing both environmental and socioeconomic data. By scrutinizing thirteen distinct machine learning algorithms, we endeavor to pinpoint the most accurate model for categorizing Italian provinces as either above or below the national average SMR value for respiratory cancer. Furthermore, employing XAI techniques, we delineate the salient factors crucial in predicting the two classes of SMR. Through our machine learning scrutiny, we illuminate the environmental and socioeconomic factors pertinent to mortality in this disease category, thereby offering a roadmap for prioritizing interventions aimed at mitigating risk factors. [ABSTRACT FROM AUTHOR]

Background: Selective androgen receptor modulators (SARMs) are small synthetic drug molecules that are still not approved as medicine in Europe or the United States but are sold on illegal websites to improve sport performance, particularly bodybuilding. Aim: To address the quality issues of illegal SARM products and their increasing diffusion in Italy with their potential health risks for consumers. Methods: Web-based tools were used to investigate retail websites, trending searches, and information exchange via social media. Thirteen SARM products, purchased on retail websites accessible from Italy, were subject to visual inspection and chemical analysis by mass spectrometry and quantitative nuclear magnetic resonance. Outcomes: The primary outcome was demonstration of additional health risks due to the illicit presence of other active ingredients, contamination, and misdosage in SARM products sold on the internet. The secondary outcome was to show the increasing trend of interest in Italy for these products. Results: Most websites reported misleading information; specifically, the statement "for research only" was reported notwithstanding indications on dosage and training phases. The trending search showed that interest toward SARMs increased in Italy in the last years. The use of these products is clearly encouraged by the emerging phenomenon of "broscience" as revealed in socials. Visual inspection evidenced nonconform labeling. Qualitative analysis confirmed the presence of the stated SARM in about 70% of samples. In 23% of samples, the expected SARM was not detected but a different one instead, and in 1 sample, no SARMs were detected. Other undeclared pharmaceutical substances (tamoxifen, clomifene, testosterone, epimethandienone, tadalafil) were measured in 30% of samples. The copresence of >1 active substance was observed in >60% of samples. Quantitative nuclear magnetic resonance data showed nonuniform content ranging from 30% to 90% of the label claim. Clinical Implications: The use of SARMs, in the presence of unexpected life-threatening reactions in persons using the products to increase sport performance, should be assessed. Strengths and Limitations: This investigation involved an integrated approach to study SARM products and related sociologic aspects. The main shortcomings are the limited number of samples and retail websites in the clear web investigated. Conclusion: SARMs sold online as food supplement–like products represent a health hazard due to the presence of unapproved and undeclared active substances. The presence of contaminants clearly indicates the absence of good manufacturing practices in the production, which increases the health risks. [ABSTRACT FROM AUTHOR]

• The whole world is plagued by the problem of missing persons. • The recovery of a missing person can benefit from the use of forensic science. • Age progression can help in the search of missing person. • The Italian Missing Children association realizing age progression for Missing Children Organization: the collaboration with Missing Child Kenya. The age progression technique allows to update identification photos of individuals who have been missing for years. This method is based on the study of the facial variation and on the dynamics of facial growth. Knowledge about changes in the distribution of facial fat and the tone of the cartilage allows realistic predictions of the facial appearance after many years. Beside these elements, age progression is also supported by similar characters between family members. This is why photos of the missing and family members are used to create an age progression, making the new identikit accurate. Non-profit associations are crucial for the dissemination of information on the problem of missing persons. In Italy, the Italian Missing Children association provides for free an age progression service with the support of forensic anthropology. With the activation of a protocol, consisting of guidelines, and a form for the request for age progression by family members, the Italian Missing Children has started a collaboration with Missing Child Kenya. It allowed to disseminate up-to-date photos of the presumed current appearance of the missing children in Kenya. Thus, in the present study some cases are reported, in which age progression technique was applied. The photos provided by Italian Missing Children were shown in video documentaries made by Missing Child Kenya, allowing to continue the dissemination of age progression photo during the Covid quarantines and to keep the population active in the search for the missing. [ABSTRACT FROM AUTHOR]

The interspecific transmission of pathogens can occur frequently in the environment. Among wild bees, the main spillover cases are caused by pathogens associated with Apis mellifera, whose colonies can act as reservoirs. Due to the limited availability of data in Italy, it is challenging to accurately assess the impact and implications of this phenomenon on the wild bee populations. In this study, a total of 3372 bees were sampled from 11 Italian regions within the BeeNet project, evaluating the prevalence and the abundance of the major honey bee pathogens (DWV, BQCV, ABPV, CBPV, KBV, Nosema ceranae, Ascosphaera apis, Crithidia mellificae, Lotmaria passim, Crithidia bombi). The 68.4% of samples were positive for at least one pathogen. DWV, BQCV, N. ceranae and CBPV showed the highest prevalence and abundance values, confirming them as the most prevalent pathogens spread in the environment. For these pathogens, Andrena, Bombus, Eucera and Seladonia showed the highest mean prevalence and abundance values. Generally, time trends showed a prevalence and abundance decrease from April to July. In order to predict the risk of infection among wild bees, statistical models were developed. A low influence of apiary density on pathogen occurrence was observed, while meteorological conditions and agricultural management showed a greater impact on pathogen persistence in the environment. Social and biological traits of wild bees also contributed to defining a higher risk of infection for bivoltine, communal, mining and oligolectic bees. Out of all the samples tested, 40.5% were co-infected with two or more pathogens. In some cases, individuals were simultaneously infected with up to five different pathogens. It is essential to increase knowledge about the transmission of pathogens among wild bees to understand dynamics, impact and effects on pollinator populations. Implementing concrete plans for the conservation of wild bee species is important to ensure the health of wild and human-managed bees within a One-Health perspective. [ABSTRACT FROM AUTHOR]

Simple Summary: This case report describes the first case of caseous lymphadenitis in a roe deer (Capreolus capreolus, Linnaeus 1758) found dead in Italy, attributable to Corynebacterium pseudotuberculosis isolated from abscessual and non-abscessual lesions of the lungs and lymph nodes. In addition, histological examinations revealed a severe parasitological infestation by bronchopulmonary larvae at various stages of development. These findings raise the possibility of the reservoir-like role of cervids for C. pseudotubercolosis and the potential implications related as much to wildlife conservation as to pathologies at the domestic/wildlife interface. Caseous lymphadenitis is a chronic debilitating disease typical of small ruminants, but it is also noted in several other domestic and wild species. In this report, we present the first documented case in Italy of pseudotuberculosis in a roe deer (Capreolus capreolus, Linnaeus 1758) found dead in the mountains of Forlì-Cesena province, Emilia Romagna region. The carcass underwent necropsy according to standard protocols, revealing generalized lymphadenopathy and severe apostematous pneumonia with multifocal and encapsulated abscesses. Corynebacterium pseudotuberculosis was isolated from the lung parenchyma, lymph nodes and abscesses. Additionally, severe parasitic bronchopneumonia of the caudal lobes and gastrointestinal strongyle infestation were detected. To our knowledge, this is the first documented case of CLA referable to C. pseudotubercolosis in a roe deer in Italy. [ABSTRACT FROM AUTHOR]

The Ansanto Valley's Mefite, one of the Earth's largest non‐volcanic CO2 gas emissions, is distinguished by its cold natural carbon dioxide springs. These emissions originate from the intricate tectonics and geodynamics of the southern Apennines in Italy. Known for over two millennia for its lethal concentration of CO2 and other harmful gases, the Mefite has a reputation for being toxic and dangerous. Despite its historical significance and unique geological features, there is a lack of information on the microbial diversity associated with the Mefite's gas emissions. This study presents an integrated exploration of the microbial diversity in the mud soil, using high‐throughput sequencing of 16S rRNA (Prokaryotes) and ITS2 (Fungi), alongside a geochemical site characterisation. Our findings reveal that the Mefite's unique environment imposes a significant bottleneck on microbial diversity, favouring a select few microbial groups such as Actinobacteria and Firmicutes for Prokaryotes, and Basidiomycota for Fungi. [ABSTRACT FROM AUTHOR]

Muscari comosum (L.) Mill. is a spontaneous plant that grows in the whole Mediterranean area, including the Basilicata and Puglia regions (southern Italy), where it has received inclusion in the Italian National List for Traditional Agri-Food Product (TAP). The food and medicinal uses of bulb are ancient due to its antioxidant properties and high variety of nutrients, such as starch, sugars, and minerals. Muscari seed is characterized by morpho-physiological dormancy, and in order to achieve uniform germination, some pre-germinative treatments are needed. In this research, the effects of hydro-priming and osmo-priming, i.e., PEG 8000 and KNO3, as well as three germination temperatures (4, 10, and 20 °C), have been evaluated. In general, the average results pointed out that the pre-treatments increased the germination index (GI) by 5% and the germination percentage (GP) by 3% compared to the no-primed control. The germination temperature of 10 °C significantly reduced the median germination time (T50) by 5.4 days and the mean germination time (MGT) by 5 days compared to temperature at 4 °C. In particular, the best results were obtained by "hydro-priming treatment × 10 °C" interaction, in terms of T50 (34.9 days) and MGT (36.3 days). This combination decreased the T50 by 10.5 days and the MGT by 9.6 days compared to the "control × 4 °C" interaction. Pearson's correlation matrix results highlighted a significant positive link between T50 and MGT (r = 0.993). In conclusion, these techniques enhanced the germination potential so that the use of pre-treated seeds could be included in a cultivation protocol to improve the germination phase and satisfy the growing demand for Italian bulbs. [ABSTRACT FROM AUTHOR]

Successful identity integration processes are fundamental for the well-being of working parents during stressful circumstances. In two studies we analyzed the associations between Parent–Work Identity Integration (PWII), workload, care-load, and parents' work problems in a sample of individual working parents in Italy (N = 326 individuals; Study 1), and those between work–life conflict, task-sharing between partners, PWII, and parents' intention to leave their job and mental health in a sample of mixed-sex working couples with school-aged children (N = 97 couples, Study 2). Finally, we examined gender differences in all the above associations. Study 1 results showed that parents dissatisfied with care-load distribution within the couple and those with higher workload also reported more problems at work, and these effects were fully mediated by PWII. Moreover, Study 2 showed that care-load distribution is only associated with women's PWII and, indirectly, their well-being and their intention to leave their job. Moreover, parents' lower work-life conflict was associated with higher PWII and, in turn, to better mental health and lower intention to leave their job. The effects of work-life conflict were also found to cross-over onto the partner's well-being. Promoting working parents' ability to harmoniously blend their work and parental identities, in addition to balance conflicting demands between work and life, appears crucial for intervention. [ABSTRACT FROM AUTHOR]

Perception of domestic and extra‐domestic environment plays a key role in structuring reality, representing the boundary between private and public life. COVID‐19 pandemic led to changes both inside and outside domestic borders, modifying those reciprocal perceptions. The aim of this study was to assess perceptions of domestic and extra‐domestic environments after the first wave of COVID‐19 pandemic on a representative Italian sample, and how such perceptions were related to individuals' well‐being at individual, family and social levels. Participants filled an online self‐report questionnaire in July 2020. Cluster analyses were conducted on domestic and extra‐domestic environment perceptions, leading to the identification of four clusters. Clusters were compared in terms of sociodemographic characteristics, and individual, family and social factors. Identified clusters report different levels of well‐being at each level. The role of factors is identified, and possible implications are discussed. Please refer to the Supplementary Material section to find this article's Community and Social Impact Statement. [ABSTRACT FROM AUTHOR]

Polychlorinated biphenyls (PCBs) are persistent organic pollutants and endocrine disruptors that have been implicated in potential damage to human semen. However, the studies conducted so far provide contrasting results. Our study aimed to investigate the associations between PCB serum and semen levels and semen quality in high school and university students living in a highly PCB-polluted area of Italy. Subjects with a normal body mass index who did not make daily use of tobacco, alcohol, drugs, or medication were selected. All participants provided a fasting blood and a semen sample. Gas chromatography-mass spectrometry was used to determine the concentrations of 26 PCB congeners. The concentrations of PCB functional groups and total PCBs were also computed. A total of 143 subjects (median age 20, range 18–22 years) were enrolled. The median total PCB concentrations were 3.85 ng/mL (range 3.43–4.56 ng/mL) and 0.29 ng/mL (range 0.26–0.32 ng/mL) in serum and semen, respectively. The analysis of the associations between sperm PCB concentration and semen parameters showed (a) negative associations between some PCB congeners, functional groups and total PCBs and sperm total motility; (b) negative associations of total PCBs with sperm normal morphology; and (c) no association of PCBs with sperm concentration. Subjects at the highest quartile of semen total PCB concentration had 19% and 23% mean reductions in total motility and normal morphology, respectively, compared to those at the lowest quartile. The analysis of the associations of serum PCB levels with sperm parameters yielded null or mixed (some positive, other negative) results. In conclusion, the present study provides evidence of a negative effect of some PCB congeners and total PCBs in semen on sperm motility and normal morphology. However, the associations between the concentration of serum and semen PCB congeners and functional groups and sperm quality parameters were inconsistent. [ABSTRACT FROM AUTHOR]

To preserve male fertility after diagnosis of any kind of cancer, a prompt assessment of the semen quality and an appropriate semen cryopreservation must be performed before radio-chemotherapy starts. The present work aims to evaluate the semen parameters at diagnosis of different cancer patients before cryopreservation and after thawing. Testicular tumors and lymphomas are among the most common cancers in younger patients, and while chemotherapy significantly increases patients' survival, it can epigenetically alter the semen fluid, resulting in temporary or permanent infertility. We analyzed data from the database of the Gamete Cryopreservation Center (Annunziata Hospital, CS; Italy) in the period of 2011–2020 from a cohort of 254 cancer patients aged 18–56 years. The evaluation was performed in a blind manner and anonymously recovered; the main parameters referring to semen quality were assessed in accordance with the WHO guidelines and decision limits (6th edition; 2021). The cancer types were as follows: testis cancers (TC; n = 135; 53.1%), hematological cancers (HC; n = 76; 29.9%), and other types of cancer (OC; n = 43; 17%). Comparing TC vs. HC (P1) and vs. OC (P2), TC had the worst semen quality: sperm number/mL (P1 = 0.0014; P2 = 0.004), total motility (P1 = 0.02; P2 = 0.07), progressive motility (P1 = 0.04; P2 = 0.05), viability (P1 = 0.01; P2 = 0.02), and percentage of atypical morphology (P1 = 0.05; P2 = 0.03). After semen thawing, viability and progressive motility recovery lowered, accounting for 46.82% and 16.75%, respectively, in the whole cohort; similarly, in the subgroups ascribed to TC, they showed the lowest recovery. Strong correlation existed between pre- and post-cryopreservation viability and progressive motility in the whole cohort (p < 0.001) and in the TC subgroup (p < 0.05). All cancer subgroups, to significantly different extents, had semen findings below the WHO reference values, suggesting diverse sperm susceptibilities to different cancers and cryodamage. Cancer and associated treatments epigenetically affect patients' semen quality, meaning cryopreservation should be considered a useful personalized prerogative for any kind of cancer in a timely manner. [ABSTRACT FROM AUTHOR]

Several animal species have been found to be susceptible to SARS-CoV-2 infection. The occurrence of infection in dogs and cats living in close contact with owners deserves particular attention from public health authorities in a One Health approach. In this study, we conducted serological screening to identify SARS-CoV-2 exposure in the sera from dogs and cats in three regions of southern Italy sampled during the years 2021 and 2022. We collected 100 serum samples in 2021 (89 from dogs and 11 from cats) and 640 in 2022 (577 from dogs and 63 from cats). Overall, the ELISA positivity rate was found to be 2.7% (20/740), with higher seroprevalence in dogs. Serum neutralization tests confirmed positivity only in two samples collected from dogs, and the assays, performed with serologically distinct SARS-CoV-2 variants, showed variant-specific positivity. This paper shows that monitoring SARS-CoV-2 exposure in animals might be affected by the viral antigenic evolution, which requires continuous updates to the serological tests used. Serological surveys are useful in understanding the true extent of exposure occurring in specific animal populations, not suffering the same limitations as molecular tests, and could help in identifying the infecting virus if tests able to characterize the immune response are used. The use of variant-specific validated serological methods should always be considered in serosurvey studies in order to determine the real impact of emerging variants on animal populations and its implications for veterinary and human health, as well as to identify potential reservoirs of the virus and its evolutionary changes. [ABSTRACT FROM AUTHOR]

Background: Chronic respiratory diseases (CRDs) are common diseases with a heterogeneous distribution worldwide. Due to their impact on disability, weight assistance and pharmaceutical spending, they represent an important global burden for national health systems. However, few studies have investigated the use and consumption of inhaled drugs in real life in patients with CRDs. Objective: This study aimed to investigate the real-life consumption of health care resources of main CRDs through an analysis of the administrative databases of the local health authority (ASL) in the Puglia region (Italy). Methods: The present study is an observational study that longitudinally reviewed the administrative and health databases associated with patients' consumption of health resources between 2017 and 2018. Results: The first important finding is a marked underestimation of the true incidence of CRDs despite the search for disease-specific exemption codes. Another important result is that the real-life consumption of inhaled drugs among these patients is well below the minimum acceptable values for adherence. The most commonly used inhaled drugs, for which at least one pack was withdrawn, were inhaled steroids (61.6%), followed by the ICS/LABA combination (43.7%) and LABAs (32.4%). However, less than one-third of patients (31%) withdrew at least three packages of ICS or ICS/LABA during the year, while the percentage was reduced to less than 15% for other combinations. Another alarming finding is that only 8.4% of patients taking CRDs drugs reported at least one spirometry during the study period. Conclusions: The wide availability of computerized systems may be an important tool for increasing therapeutic adherence and optimizing the resources of health systems in the diagnosis, treatment and management of patients with CRDs. [ABSTRACT FROM AUTHOR]

Background: Although the role of viral agents, such as human papillomavirus (e.g. HPV16, HPV18) in colorectal cancer (CRC) has been previously investigated, results remain inconclusive. Methods: To further evaluate the involvement of oncogenic HPV types in CRC, 40 frozen neoplastic and 40 adjacent colonic tissues collected from Italian patients were analyzed by Luminex-based assays that detect a broad spectrum of HPV types, i.e. Alpha (n = 21), Beta (n = 46) and Gamma HPVs (n = 52). In addition, 125 frozen CRC samples and 70 surrounding mucosal tissues were collected from Czech patients and analyzed by broad spectrum PCR protocols: (i) FAP59/64, (ii) FAPM1 and (iii) CUT combined with Next Generation Sequencing (NGS). Results: Using Luminex-basedassays, DNA from HPV16 was detected in 5% (2/40) CRC tissues from Italian patients. One HPV16 DNA-positive CRC case was subsequently confirmed positive for E6*I mRNA. Cutaneous beta HPV types were detected in 10% (4/40) adjacent tissues only, namely HPV111 (n = 3) and HPV120 (n = 1), while gamma HPV168 (n = 1) and HPV199 (n = 1) types were detected in adjacent and in tumor tissues, respectively. The NGS analysis of the CRC Czech samples identified HPV sequences from mucosal alpha-3 (HPV89), alpha-7 (HPV18, 39, 68 and 70) and alpha-10 species (HPV11), as well as cutaneous beta-1 (HPV20, 24, 93, 98, 105,124) beta-2 (HPV23), beta-3 (HPV49) and gamma-1 species (HPV205). Conclusions: Our findings indicate that HPV types belonging to the mucosal alpha, and the 'cutaneous' beta and gamma genera can be detected in the colonic mucosal samples with a low prevalence rate and a low number of HPV reads by Luminex and NGS, respectively. However, additional studies are required to corroborate these findings. [ABSTRACT FROM AUTHOR]

This paper compares several methods for constructing weekly nowcasts of recession probabilities in Italy, with a focus on the most recent period of the Covid-19 pandemic. The common thread of these methods is that they use, in different ways, the information content provided by financial market data. In particular, a battery of probit models are estimated after extracting information from a large dataset of more than 130 financial market variables observed at a weekly frequency. The accuracy of these models is explored in a pseudo out-of-sample nowcasting exercise. The results demonstrate that nowcasts derived from probit models estimated on a large set of financial variables are, on average, more accurate than those delivered by standard probit models estimated on a single financial covariate, such as the slope of the yield curve. The proposed approach performs well even compared with probit models estimated on single time series of real economic activity variables, such as industrial production, business tendency survey data or composite PMI indicators. Overall, the financial indicators used in this paper can be easily updated as soon as new data become available on a weekly basis, thus providing reliable early estimates of the Italian business cycle. [ABSTRACT FROM AUTHOR]

Livestock European diffusion followed different human migration waves from the Fertile Crescent. In sheep, at least two diffusion waves have shaped the current breeds' biodiversity generating a complex genetic pattern composed by either primitive or fine-wool selected breeds. Nowadays most of the sheep European breeds derive from the second wave which is supposed to have largely replaced oldest genetic signatures, with the exception of several primitive breeds confined on the very edge of Northern Europe. Despite this, some populations also in the Mediterranean region are characterised by the presence of phenotypic traits considered ancestral such as the policeraty, large horns in the ram, short tail, and a moulting fleece. Italy is home of a large number of local breeds, albeit some are already extinct, others are listed as critically endangered, and among these there is the Quadricorna breed which is a four-horned sheep characterised by several traits considered as ancestral. In this context we genotyped 47 individuals belonging to the Quadricorna sheep breed, a relict and endangered breed, from Central and Southern Italy. In doing so we used the Illumina OvineSNP50K array in order to explore its genetic diversity and to compare it with other 41 breeds from the Mediterranean region and Middle-East, with the specific aim to reconstruct its origin. After retaining 32,862 SNPs following data filtering, the overall genomic architecture has been explored by using genetic diversity indices, Principal Component Analysis (PCA) and admixture analysis, while the genetic relationships and migration events have been inferred using a neighbor-joining tree based on Reynolds' distances and by the maximum likelihood tree as implemented in treemix. The Quadricorna breed exhibit genetic diversity indices comparable with those of most of the other analysed breeds, however, the two populations showed opposing patterns of genetic diversity suggesting different levels of genomic inbreeding and drift (FIS and FROH). In general, all the performed genome-wide analyses returned complementary results, indicating a westward longitudinal cline compatible with human migrations from the Middle-East and several additional genetic footprints which might mirror more recent historical events. Interestingly, among the Italian breeds, the original Quadricorna (QUAD_SA) first separated showing its own ancestral component. In addition, the admixture analysis does not suggest any signal of recent gene exchange with other Italian local breeds, highlighting a rather ancestral purity of this population. On the other hand, both the neighbor-joining tree and the treemix analysis seem to suggest a proximity of the Quadricorna populations to breeds of South-Eastern Mediterranean origin. Although our results do not support a robust link between the genetics of the first wave and the presence of primitive traits, the observed genetic uniqueness together with the inferred phylogeograpic reconstruction would suggest an ancient presence of the Quadricorna breed in the Italian Peninsula. Because of this singularity, urgent conservation actions are needed in order to keep the breed and all related cultural products alive. [ABSTRACT FROM AUTHOR]

The aim of this research is the identification of geomatics techniques and methods capable of representing historic centres in a rapid, detailed and accurate manner. Indeed, historic centres are a cultural heritage asset to be preserved, protected and conserved for future generations, taking into account the history of a community. The paper describes a geomatics methodology applied to the case study of Capitignano (Italy), one of the municipalities that was affected by the 2009 earthquake that involved numerous historic centres causing extensive damage, not only in terms of human lives but also to the cultural heritage. The integration of geomatics techniques made it possible to obtain a georeferenced point cloud that is particularly useful for the description of buildings in historic centres, both at the urban scale and at the scale of individual artefacts. In addition, it was also possible to produce orthophotos with a high geometric resolution that made it possible to identify lesions in structures quickly and accurately. [ABSTRACT FROM AUTHOR]

AIM: The study aimed to analyze students' approach to evidence-based practice and evaluate the effect of teaching (based on lessons in statistics, epidemiology, evidence-based nursing, bioengineering, English language) and internship experiences on the attitude of nursing students to evidence-based practice. METHOD: In 2019, one hundred nineteen second-year nursing students from an Italian university were involved in a pre-post longitudinal study. The Italian version of the Student-Evidence Based Practice Questionnaire was administered to nursing student in four moments of one academic year. RESULTS: The Student-Evidence Based Practice Questionnaire showed different mean scores based on the four moments of the survey with a significant increase after the experience of planning clinical cases with an online simulation system. In particular, the aptitude subscale does not have significant improvements in the various phases of the study. Conversely, the support of the clinical tutor affects student performance. The didactic module on evidence-based nursing develops the ability to assess the quality of the information found and its sharing. Basic English proficiency is not significantly correlated. CONCLUSION: To develop evidence-based practice knowledge, aptitude, and skills in nursing students, it is necessary to strengthen the learning opportunities both in classroom lessons and in simulation and internship experiences. [ABSTRACT FROM AUTHOR]

Background: Smith-Magenis syndrome (SMS) is caused by either interstitial deletions in the 17p11.2 region or pathogenic variants in the RAI1 gene and is marked by a distinct set of physical, developmental, neurological, and behavioral features. Hypercholesterolemia has been described in SMS, and obesity is also commonly found. Aim: To describe and characterize the metabolic phenotype of a cohort of SMS patients with an age range of 2.9–32.4 years and to evaluate any correlations between their body mass index and serum lipids, glycated hemoglobin (HbA1c), and basal insulin levels. Results: Seven/thirty-five patients had high values of both total cholesterol and low-density lipoprotein cholesterol; 3/35 had high values of triglycerides; none of the patients with RAI1 variants presented dyslipidemia. No patients had abnormal fasting glucose levels. Three/thirty-five patients had HbA1c in the prediabetes range. Ten/twenty-two patients with 17p11.2 deletion and 2/3 with RAI1 variants had increased insulin basal levels. Three/twenty-three patients with the 17p11.2 deletion had prediabetes. Conclusion: Our investigation suggests that SMS 'deleted' patients may show a dyslipidemic pattern, while SMS 'mutated' patients are more likely to develop early-onset obesity along with hyperinsulinism. [ABSTRACT FROM AUTHOR]

Cortesognoite (IMA 2014-029), CaV2Si2O7(OH)2·H2O, is a new vanadium silicate mineral that occurs within V-bearing lawsonite in association with vanadiocarpholite, chalcocite, quartz, minor poppiite, roscoelite, vanadomalayaite and volborthite in a silicified wood hosted in Mn-ore-bearing metacherts from the Molinello manganese mine in the Graveglia Valley, Northern Apennines, Liguria, Italy. The mean chemical composition of type cortesognoite by electron probe microanalysis is (wt%) SiO2 34.33, V2O3 31.38, CaO 15.80, Al2O3 7.69, MnO 0.14, FeO 0.09, MgO 0.06, TiO2 0.02 and H2O 10.29, totaling 99.80, giving rise to an empirical formula of (Ca0.99Mn0.01)(V1.47Al0.53Mg0.01)Si2.00O7(OH)2·H2O. The end-member formula is CaV2Si2O7(OH)2·H2O. Cortesognoite has the Cmcm lawsonite structure with a = 5.85(1) Å, b = 8.79(1) Å, c = 13.13(1) Å, V = 675(1) Å3 and Z = 4 as revealed by electron back-scatter diffraction. The calculated density using the measured composition is 3.44 g/cm3. Cortesognoite is a secondary alteration phase, formed with V-bearing lawsonite by multi-stage hydrothermal processes that occurred in the silicified fossil wood. The mineral name is in honor of Luciano Cortesogno, professor of petrography at University of Genova, Italy. [ABSTRACT FROM AUTHOR]

Due to recent events, professional liability for psychiatrists in Italy is currently a matter of lively debate. Specifically, overwhelming pressure on psychiatrists’ duties has been brought by regulatory developments, such as the closure of forensic psychiatric hospitals, with the consequent return of offenders to communitybased care, and the mental health consequences of the pandemic. According to Italian courts, psychiatrists are not only responsible for diagnostic and therapeutic appropriateness but also for the effects of their interventions on patients, and their behaviors. The aim of this study was to explore the attitude and behaviors of Italian psychiatrists regarding defensive medicine and professional liability. A total sample of 254 psychiatrists was surveyed by means of a quantitative online questionnaire. Most psychiatrists reported practicing defensive medicine (no. 153/254, 60.2%) and felt that their position of guarantee compromised their work in healthcare for patients (no. 138/253, 54.3%). Age correlated inversely with acknowledgment of defensive practices (r  = −0.245, p  <  0.001), with younger physicians more prone to defensive medicine (p  =  0.013), particularly for patients at risk of suicide or violence. Psychiatrists in ‘closed’ settings (hospital wards, residential and rehabilitation centers, mental health service units in prison) reported more malpractice claims (p  =  0.037) and complaints (p  =  0.031), as well as a greater propensity to act defensively. In the treatment of patients with violent behavior, suicidal ideation, dual diagnoses, and criminal convictions, defensive practices were associated more with perceived legal risks (r  =  0.306, p  <  0.001) than actual legal involvement (p  >  0.05). Anxiety, anger, and restlessness were common reactions to legal complaints, involving no. 50/254 (19.7%) respondents, with 40% reporting impaired functioning. Most psychiatrists (no. 175/253, 68.9%) were concerned about both civil and criminal laws regarding their professional responsibility, but many were not fully informed about recent legislative regulations and younger physicians resulted scarcely trained in risk management (p  <  0.001). In conclusion, our findings suggest that defensive medicine is a common phenomenon among psychiatrists and their position of guarantee drives this attitude. Education on legal implications and risk management should be provided starting from the university and continuing over time, to improve the knowledge of young and senior doctors on professional liability and inform their decision-making processes. This would also reduce defensive practices and improve the quality of healthcare. Considering the concerns of younger physicians, as well as of professionals working in acute and high-intensity medical care facilities, there is also an urgent need for a revision of the medical liability to ensure the sustainability of the National Health Service. [ABSTRACT FROM AUTHOR]

The role of migratory birds in the spread of ticks and tick-borne pathogens along their routes from Africa to Europe is increasingly emerging. Wild birds can host several tick species, often infected by bacteria responsible for zoonoses. The aim of the study is to assess the possible introduction of exotic ticks carried by migratory birds into Italy from Africa and to detect the presence of Rickettsia species and Coxiella burnetii they may harbor. During a two-year survey, we collected ticks from migratory birds captured during their short stop-over on Ventotene Island. Specimens were first identified by morphology or sequencing molecular targets when needed, and then tested by real-time PCR for the presence of selected pathogens. A total of 91% of the collection consisted of sub-Saharan ticks, more than 50% of which were infected by Rickettsia species belonging to the spotted fever group, mainly represented by R. aeschlimannii. In contrast, the suspected C. burnetii detected in two soft ticks were confirmed as Coxiella-like endosymbionts and not the pathogen. Although there are still gaps in the knowledge of this dispersal process, our findings confirm the role of migratory birds in the spread of ticks and tick-borne pathogens, suggesting the need for a continuous surveillance to monitor the potential emergence of new diseases in Europe. [ABSTRACT FROM AUTHOR]

Background Analyses of coronavirus disease 19 suggest specific risk factors make communities more or less vulnerable to pandemic-related deaths within countries. What is unclear is whether the characteristics affecting vulnerability of small communities within countries produce similar patterns of excess mortality across countries with different demographics and public health responses to the pandemic. Our aim is to quantify community-level variations in excess mortality within England, Italy and Sweden and identify how such spatial variability was driven by community-level characteristics. Methods We applied a two-stage Bayesian model to quantify inequalities in excess mortality in people aged 40 years and older at the community level in England, Italy and Sweden during the first year of the pandemic (March 2020–February 2021). We used community characteristics measuring deprivation, air pollution, living conditions, population density and movement of people as covariates to quantify their associations with excess mortality. Results We found just under half of communities in England (48.1%) and Italy (45.8%) had an excess mortality of over 300 per 100 000 males over the age of 40, while for Sweden that covered 23.1% of communities. We showed that deprivation is a strong predictor of excess mortality across the three countries, and communities with high levels of overcrowding were associated with higher excess mortality in England and Sweden. Conclusion These results highlight some international similarities in factors affecting mortality that will help policy makers target public health measures to increase resilience to the mortality impacts of this and future pandemics. [ABSTRACT FROM AUTHOR]

Infestations of Arge scita (Mocsáry, 1880) were recorded for the first time in Italy in summer 2022. Almond trees showing severe leaf damages, caused by the sawfly larvae feeding on the leaf lamina were recorded in the Salento peninsula of the Apulia region (southern Italy). Severely infested trees had a defoliated looking-appearance, with the lamina of the whole foliage completely consumed, with only the thick middle vein and petioles remaining attached to the trees. The occurrence of A. scita on the infested trees was confirmed by molecular and morphological characterization of specimens of larvae at different stages and adults. The simultaneous presence on the infested trees of eggs, larvae of different ages, cocoons and adults suggests that most likely this sawfly in our environmental conditions behave as a multivoltine species. Further investigations and surveys are needed to assess the live cycle and the host range, as well as to understand whether this phytophagous could represent a serious threat in areas where almond trees are cultivated. [ABSTRACT FROM AUTHOR]

Summary: Splenectomy/asplenia is a condition associated with immune‐compromission and specific vaccines are recommended for these patients, including the anti‐COVID‐19 vaccine. Among the high‐risk group for which vaccination was prioritized in Italy, the immunocompromised patients after therapies or treatments were included. The Apulian regional archive of hospital discharge forms was used to define the list of splenectomized Apulian inhabitants, considering data from 2015 through 2020. The overall vaccination status of asplenic patients was assessed via data collected from the Regional Immunization Database. The history of SARS‐CoV‐2 infection and the infectious disease outcomes were extracted from the Italian Institute of Health platform "Integrated surveillance of COVID‐19 cases in Italy". 1219 Apulian splenectomized inhabitants were included; the incidence rate of SARS‐CoV‐2 infection was 15.0 per 100 persons‐year with a proportion of re‐infection equal to 6.4%; the proportion of hospitalization was 2.9%, with a case‐fatality rate of 2.6%. The vaccine coverage (VC) for the anti‐COVID‐19 vaccine basal routine was 64.2%, for the first booster dose was 15.4%, and for the second booster dose was 0.6%. A multifactorial approach is needed to increase the vaccination uptake in this sub‐group population and to increase the awareness of the asplenia‐related risks to patients and health personnel. [ABSTRACT FROM AUTHOR]

Many ants and hemipterans are bound by a mutualistic relationship (trophobiosis) which represents an ecological keystone: ants receive food (honeydew) providing different kinds of protection. Partner protection against arthropod natural enemies is considered to be frequent and to interfere with biological control strategies of hemipteran pests.We carried out an ant‐exclusion field experiment in a fava bean (Vicia faba) plantation in Italy to monitor the abundance and behaviour of the ants (Plagiolepis pygmaea, Tetramorium semilaeve and the exotic Nylanderia jaegerskioeldi) and their relationship with facultative mutualist (Aphis fabae) and non‐mutualist (Megoura viciae) aphids, the arthropod natural enemies of the aphids, and extrafloral nectaries.Ants concentrated their activity on the attendance of facultative mutualist aphids much more than on the extrafloral nectaries. The ant‐exclusion treatment had no effect on the abundance of M. viciae and on the parasitization rate of A. fabae, while it reduced the abundance of A. fabae, aphid predators, and aphid parasitoids.Our results demonstrate that ant attendance does not imply the protection of aphids from arthropod natural enemies and suggest that the identity of the ant species involved is important for the outcome. As relatively few species have been studied in this regard, extending our knowledge to the role of more ant species is desirable to understand the ecology and evolution of ant‐aphid mutualisms and to refine integrated control strategies of aphid pests. [ABSTRACT FROM AUTHOR]

Simple Summary: Wildlife monitoring plans are not uniformly applied in all European countries, even if the World Organization for Animal Health (WOAH) identified passive surveillance of wildlife as the strategy of choice to investigate the health status of wild animals. The plan in use in the Emilia-Romagna region (Northern Italy) for the past 11 years has allowed for the collection of a large amount of data on the wild boar population. Research has been conducted on diseases for which the wild boar could be a reservoir and/or source of infection for domestic pigs due to their increasingly frequent interfaces (trichinellosis, tuberculosis, brucellosis, african swine fever, classical swine fever, Aujeszky's disease, swine vesicular disease, and swine influenza A). Although the results do not allow us to make inferences about the resident population due to the sampling method and sample size, they still give us some indications about the strengths and weaknesses of the plan itself. For instance, an active search for carcasses on the territory should be implemented. In order to improve surveillance activities, it would also be desirable to increase the harmonization of sample collection schemes and data organization from a One Health perspective, as recommended by the WOAH. In recent years, the growth of wild ungulates has increased the focus on their health monitoring. In particular, the health status of wild boars is relevant for the economic impact on the pig industry. The Emilia-Romagna region activated a wildlife monitoring plan to better evaluate the health status of the wild boar population. Between 2011 and 2021, samples of found dead and hunted wild boar have been examined for trichinellosis, tuberculosis, brucellosis, african swine fever, classical swine fever, Aujeszky's disease, swine vesicular disease, and swine influenza A. Trichinella britovi was identified in 0.001% of the examined wild boars; neither M. bovis nor M. tuberculosis were found in M. tuberculosis complex positive samples; 2.3% were positive for Brucella suis; 29.4% of the sera were positive for Aujeszky's disease virus; and 0.9% of the samples were positive for swine influenza A virus. With an uncertain population estimate, the number of animals tested, the number of positives, and the sampling method do not allow us to make many inferences but suggest the need to implement and strengthen the existing surveillance activity, as it seems to be the only viable alternative for safeguarding animal and human health. [ABSTRACT FROM AUTHOR]

The European wildcat is an elusive small carnivore species whose distribution, behavioural ecology and interactions with domestic cats are scantly known. However, the use of camera-trapping is steadily increasing in wildlife studies as well as citizen science, with the latter setting the basis for a large source of robust data. Here we provide an overview of our efforts to create an independent network, named Piccoli Fototrappolatori Indipendenti (Little Independent Camera-trappers, hereafter PFI), of citizen scientists who are contributing with the goal of a deeper understanding of wildcat ecology. We engaged 31 volunteers who collected domestic cats, putative hybrids (hereafter hybrids) and wildcats' detections at 503 locations throughout Italy from 11/04/2006 to 24/10/2022. So far, this dataset hosts 312 images and 1015 videos (1327 detections) which were morphological examined and standardised, leading to 123, 137 and 1016 detections of domestic cats, hybrids and wildcats, respectively. We documented the expansion of the wildcat towards Northern Italy, with the first camera-trapping records from the Western Alps (Val D'Aosta) and from the Northern Apennines (Liguria), as well as the detection of kink-tailed wildcats in new regions. Moreover, we observed behavioural differences among cat types, with domestic cats marking at a lower rate and with hybrids being less elusive than wildcats at night. Further research and efforts are needed to better explore the conservation consequences of our findings, as well as to investigate the full potential of citizen science combined with camera trapping which are promising tools in wildcat conservation. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this paper is to provide an analysis of the present situation and recent evolution of entrepreneurship education in Italian universities and to discuss whether these courses and curricula match the demand for entrepreneurial competences. Design/methodology/approach: The empirical analysis is based on a census of entrepreneurship courses and curricula run by universities. The information collected through the internet refers to the academic years 2003-2004 and 2009-2010. Findings: Compared with the situation observed in the USA and in other European countries, entrepreneurship education in Italy is rather "underdeveloped". Only a few universities have courses or specific curricula dedicated to entrepreneurship. The courses are concentrated within business faculties while very few exist in science and engineering faculties. The slow pace with which Italian universities are keeping up with the global trend in entrepreneurship education at university level seems in vivid contrast with the need for the Italian economy to change its industry structure from the so-called "traditional" to "high-tech" sectors. The paper discusses the reasons for this situation. Research limitations/implications: The paper does not evaluate the impact of entrepreneurship education. A suggestion for future research could be to analyze the role of these courses in encouraging entrepreneurial activity of students. Practical implications: Entrepreneurship education at university level can play an important role in the Italian economic system, fostering the creation of new business in knowledge-intensive sectors. Social implications: The exploratory analysis of the state of entrepreneurship education in Italy suggests the need to develop these courses and spread the presence, especially in the science and engineering universities. Originality/value: The paper covers a lack of research on the attitude of higher education institutions towards entrepreneurship education in Italy. (Contains 5 tables and 8 notes.)

Lettuce crop is an important horticultural crop of several Mediterranean countries, including Italy. The Italian region which is a major producer of lettuce crops is Apulia, where this crop is cultivated in open fields an in greenhouses. Since several microbial pathogens are responsible for important diseases found on lettuce produced in greenhouses, in this study, the experimental activities focused on the most severe fungal soilborne pathogens, i.e., Sclerotinia sclerotiorum and Athelia rolfsii. Their control is often performed with fungicides which cause public concern over the environment and human health. The main aims of this study were to determine the biocontrol efficacy of a Streptomyces strain in vitro and in vivo conditions on lettuce seedlings against Athelia rolfsii and Sclerotinia sclerotiorum as severe fungal soilborne pathogens through the application of its vegetative propagules and putative bioactive crude extracts via filtrate culture. The results obtained confirm a significant effectiveness of CARA17 strain to control the severity of both fungal soilborne pathogens during two different experiments: when it is used as vegetative propagules and as a culture filtrate containing putative bioactive metabolites in vitro and in vivo conditions. These preliminary results demonstrated that the actinomycetes CARA17 strain is valid as a biocontrol agent (BCA) against both the severe phytopathogens used in this study. The biocontrol action performed from the CARA17 strain is clearly and mainly due to the putative bioactive crude extracts produced, but further studies are necessary to identify which metabolites (polyphenols, terpenes, fatty acids, etc.) are produced from this Streptomyces strain. [ABSTRACT FROM AUTHOR]

Purpose: Along the coronavirus pandemic, huge business challenges are facing as a result of collapsing customer demand and organisational significant changes supported by digital development, while the increasing social and environmental needs involve business and individuals. The authors argue that this trend is modifying organisational and market logic, replacing them with values and practices linked to community-based models. The present work aims to study the impact that smart working (SW) has on the worker, seen both as a member of the organisation and the social community. Design/methodology/approach: The study data were collected from a computer-assisted web interview administered in 2020 to public employees working for health agencies across the Campania region, in South Italy. To test the conceptual model, partial least squares-structural equation modelling is used. Considering the abductive soul of the research, the study represents a pilot survey that will deliver stochastic results to be subsequently replicated in all Italian health agencies. Findings: The results of the research highlighted how the evolutionary dynamics of SW employees tend towards a reconceptualisation of workspaces, a redefinition of time and emotions and a better balance between work and personal life, thus creating a greater space for social and community aspects and determining a greater involvement in their working life. Originality/value: This research introduces a new win-win logic in the labour market, one capable of generating advantages for people, organisations and the entire social system by allowing workers to better reconcile working times with their personal needs and with flexibility demands coming from companies. [ABSTRACT FROM AUTHOR]

Canine Vector-Borne Diseases (CVBDs) are widespread in Europe and enzootic in many other countries. Though severe illnesses may occur, dogs living in enzootic areas often show vague or no clinical signs of CVBDs. Undiagnosed infections/co-infections in subclinically infected animals favor the spread of CVBDs and increase the risk of transmission to other animals and, in some cases, humans. This study has evaluated the exposure of dogs living in key enzootic countries, i.e., Italy and Greece, to major CVBDs via the use of in-clinic diagnostic kits. Overall, 300 privately owned dogs without/with single mild clinical signs living in different regions of Italy (n. 150) and Greece (n. 150) were included in the study. As part of a clinical examination, a blood sample was collected from each dog and subjected to two serological rapid tests, i.e., the SNAP® 4Dx®Plus (IDEXX Laboratories Inc.) for the detection of antibodies against Ehrlichia spp., Anaplasma spp., Borrelia burgdorferi s.l. and Dirofilaria immitis antigen and the SNAP® Leishmania (IDEXX Laboratories Inc.) for the detection of antibodies against Leishmania infantum. In all, 51 dogs (17%; 95% CI 12.9–21.7) were seropositive to at least 1 pathogen, i.e., 4 in Italy (2.7%; 95% CI 1.4–13.1) and 47 in Greece (31.3%; 95% CI 24–39.4). Dirofilaria immitis antigens were found in 39 dogs (13%; 95% CI 9.4–17.3), while antibodies against Ehrlichia, Anaplasma and Leishmania were detected in 25 (8.3%; 95% CI 5.5–12.1), 8 (2.7%; 95% CI 1.2–5.2) and 5 (1.7%; 95% CI 0.5–3.8) dogs, respectively. None of the dogs tested seropositive for B. burgdorferi s.l. Statistical analyses were performed to evaluate associations between exposure to CVBDs and possible risk factors. The present results indicate that dogs living in enzootic areas may be seropositive for one or more CVBDs in absence of clinical signs. Rapid kits are among first line tools for the detection of CVBDs in clinical settings, as they are cost-effective, straightforward and quick to use. Also, in-clinic tests used herein allowed detection of co-exposure to CVBDs investigated. [ABSTRACT FROM AUTHOR]

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