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Abstract Increasing production of energy crops in Europe, mainly maize and rapeseed, has altered patterns of pesticide use in recent decades. The long-term effects of S-metolachlor (S-M) and of its metabolite metolachlor OA (M-OA) at the environmentally relevant concentration of 4.2 μg L−1 and at 42 μg L−1 (ten-fold concentration) on marbled crayfish (Procambarus virginalis) were evaluated in a 28-day exposure and after a subsequent 28-day recovery period. Indicators assessed were behaviour; biochemical haemolymph profile; oxidative and antioxidant parameters of gill, hepatopancreas, and muscle; and histology of hepatopancreas and gill. Results showed biochemical haemolymph profile (lactate, alanine aminotransferase, aspartate aminotransferase, inorganic phosphate), lipid peroxidation in hepatopancreas, and antioxidant parameters (catalase, reduced glutathione, glutathione S-transferase) of hepatopancreas and gill of crayfish exposed to S-M and M-OA to significantly differ from controls (P < 0.01). Antioxidant biomarker levels remained different from controls after a 28-day recovery period. Differences in behaviour including speed of movement and velocity, and histopathological damage to gill and hepatopancreas were associated with S-M and M-OA exposure and persisted after 28 days in S-M- and M-OA-free water. Results suggest harmful effects of low concentrations of S-M and its metabolite M-OA on non-target organisms and provide information for assessing their effects at environmentally relevant concentrations. Highlights • S-metolachlor and its metabolite metolachlor OA are frequently detected in rivers. • Their harmful effects were studied on the non-target invertebrate at an environmentally relevant concentration. • Exposure to these compounds was associated with changes in biochemical parameters, histology, and behaviour in crayfish. • Damage was not reversed by 28 days recovery in clean water. [ABSTRACT FROM AUTHOR]

The concept of environmental flows (E-Flows) describes the streamflow that is necessary to maintain river ecosystems. Although a large number of methods have been developed, a delay was recorded in implementing E-Flows in non-perennial rivers. The general aim of the paper was to analyse the criticalities and the current state of implementation of the E-Flows in non-perennial rivers of southern Europe. The specific objectives were to analyse (i) the European Union (EU) and national legislation on E-Flows, and (ii) the methodologies currently adopted for setting E-Flows in non-perennial rivers in the EU Member States (MSs) of the Mediterranean Region (Spain, Greece, Italy, Portugal, France, Cyprus, and Malta). From the analysis of national legislations, it is possible to acknowledge a step forward toward regulatory unification at the European level, on the subject of E-Flows and more generally toward the protection of aquatic ecosystems. The definition of E-Flows, for most countries, has abandoned the idea of a regime of constant and minimal flow, but it recognizes the importance of the biological, and chemical-physical aspects connected to it. From the analysis of the E-Flows implementation through the review of the case studies, one can surmise that in non-perennial rivers the E-Flows science is still an emerging discipline. The limited availability of hydrological, hydraulic, and biological data as well as the restricted economic resources allocated for managing non-perennial rivers are the main causes of the delay in the E-Flows implementation in MSs. The results of the present study may contribute in setting an E-Flow regime in non-perennial rivers., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper, (Copyright © 2023 Elsevier Ltd. All rights reserved.)

In the last decade many papers have explored the challenges of defining and framing urban resilience. Academic literature has recently emphasised how urban resilience differs from urban sustainability, while urban policy agendas use these terms almost interchangeably. This paper explores European academics' and city practitioners' perspectives around urban resilience meanings and principles. It aims to enhance the understanding of the concept's evolution and to discuss the gap between theory and implementation. To do so, we conducted a survey among around 100 junior and senior European scholars from different fields and interviewed 24 city practitioners. Both academics' and practitioners' perspectives have aligned with the latest conceptualisations of urban resilience. The respondents endorsed transformative and "bouncing forward" approaches, emphasizing that nowadays challenges stand within reconciling and combining transformation and resistance to change resilience perspectives. The more engineering, robustness and safety driven ones seem to be left behind. However, inconsistencies emerged when addressing implementation and resilience characteristics: interviews' responses showed how practices are still driven through robustness and safety-driven measures. The paper critically discusses these emerging conceptual misalignment and gaps to overcome in future research and practice. • Perspectives on urban resilience among EU academics and practitioners are analysed. • The metaphor of urban resilience as 'bouncing forwards' has become more popular. • Conceptual misalignments emerged about resilience definition and its characteristics. • Different actors have diverging ideas about what transformative approach entails. • Better alignment between urban resilience and sustainability is needed. [ABSTRACT FROM AUTHOR]

The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (<0.1% to 38.3%), being positively correlated (p < 0.001) with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX-R coliforms, loads over 10 3 colony-forming units per mL were occasionally observed in final effluents. We demonstrate that CTX-R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status., (Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.)

This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.