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Background: Small cell lung cancer (SCLC) is characterized by aggressive spread and poor prognosis, but has limited treatment options. Results of prognostic factors from randomized trials on treatment arrangement are conflicting and large‐scale real‐world analysis is lacking. Methods: Patients diagnosed SCLC between 2008 and 2018 in Peking University Cancer Hospital were included in this study. Kaplan–Meier methods were adopted, and univariate analysis and multivariate Cox regression models were constructed to analyze prognostic factors. Results: Among 1045 patients who presented to our center, 988 eligible patients were identified. Median overall survival (OS) was 16.0 months for the whole group, 24.0 months and 11.0 months for limited stage small cell lung cancer (LS‐SCLC) and extensive stage small cell lung cancer (ES‐SCLC), separately. Limited‐stage, good performance status (PS) (ECOG 0–1), response to primary systemic treatment, and patients who received initiative irradiation and three or more lines of chemotherapy were predicted to have better OS in the whole group. Only response to first‐line systemic therapy and prophylactic cranial irradiation (PCI) were independent prognostic factors of survival in LS‐SCLC; while good PS (ECOG 0–1), without liver, bone, or subcutaneous metastases, response to first‐line therapy, initial local irradiation, and three or more lines of systemic therapy predicted a favorable prognosis in ES‐SCLC. Conclusions: The present study retrieved from large real–world data suggested that response to primary systemic therapy and aggressive radiotherapy are independent prognostic factors for SCLC. PCI and initiative irradiation for original or metastatic sites improved the OS in LS‐SCLC and ES‐SCLC, respectively. [ABSTRACT FROM AUTHOR]

Background: Programmed cell death-1/programmed cell death ligand-1 (PD-1/PD-L1) inhibitors are increasingly used in China, but no real-world data are available about the immune-related adverse events (irAEs). This real-world retrospective study aimed to assess the safety and effectiveness of PD-1/PD-L1 inhibitors in patients with non-small cell lung cancer (NSCLC) and to analyze the association between irAEs and effectiveness. Methods: This was a retrospective study of the clinical data of patients with NSCLC treated with PD-1/PD-L1 inhibitors from August 2016 to November 2019 at Beijing Cancer Hospital. The patients were divided into the irAE or non-irAE groups. Overall adverse events, the impact of irAE on tumor response, and the association of irAEs with effectiveness were evaluated. Results: One hundred and ninety-one patients were included, including 70 (36.6%) patients in the irAE group and 121 (63.4%) patients in the non-irAE group. AE, grades 3–5 AEs, and irAE occurred in 107 (56.0%), 24 (12.6%), and 70 (36.6%) of the patients, respectively. The objective response rate (ORR) and disease control rate (DCR) were higher in the irAE group compared with the non-irAE group (42.0% vs. 25.8%, P=0.038; 91.9% vs. 70.8%, P=0.002). Multivariable analyses identified that irAE were associated with progression-free survival (HR=0.62, 95%CI: 0.43–0.91; P=0.015), but not with overall survival (HR=0.76, 95%CI: 0.44–1.28; P=0.299). Conclusion: In NSCLC treated with PD-1/PD-L1 inhibitors, patients with irAEs showed improved effectiveness over patients without irAEs. Future studies of anti-PD-1/PD-L1 immunotherapy should explore this association and the underlying biological mechanisms of efficacy. [ABSTRACT FROM AUTHOR]

The purpose of this prospective phase II clinical trial was to investigate the efficacy and safety of anlotinib in patients with relapsed small cell lung cancer (SCLC). Forty‐five patients with relapsed SCLC were enrolled and treated with anlotinib (one cycle of 12 mg daily for 14 days, discontinued for 7 days, and repeated every 21 days) until disease progression or intolerance of treatment. The primary end point was progression‐free survival (PFS). Secondary end points were overall survival (OS), disease control rate (DCR), objective control rate (ORR) and toxicity. The median PFS was 4.1 months (95% confidence interval [CI] 2.4‐5.8) and the median OS was 6.1 months (95% CI 2.2‐10.0). The OS for the limited‐stage subgroup was significantly longer than that of the extensive‐stage subgroup (P =.02). The DCR was 67%, and the ORR was 11%. The most common adverse event was hypertension (13%), which was controlled well with antihypertensive drugs. In conclusion, anlotinib has likely efficacy in patients with relapsed SCLC, and the side effects can be well tolerated. A longer OS was observed in limited‐stage SCLC patients treated with anlotinib. What's new? Although untreated small cell lung cancer (SCLC) patients are usually sensitive to chemotherapy, they are prone to relapse. Anlotinib is a novel multi‐target small molecule tyrosine kinase inhibitor with both anti‐angiogenesis and anti‐tumor growth effects. While anlotinib became the first drug approved for third‐line and further‐line treatment of SCLC in China, few studies have focused on anlotinib treatment in relapsed SCLC. This one‐arm, prospective phase II clinical study reports a median progression‐free survival of 4.1 months (95% CI 2.4‐5.8) and median overall survival of 6.1 months (95% CI 2.2‐10.0) for anlotinib treatment in relapsed SCLC, with relatively mild side effects. [ABSTRACT FROM AUTHOR]

Background: Rice paddy wetlands may serve as a nutrient source or sink in agricultural ecosystems. However, the pattern of nutrient loss on a watershed scale is not clear. A year-round on-site observation study based on 6-h intervals was conducted. Rainfall, temperature, runoff nutrient concentrations, and adjacent stream water samples were automatically monitored to uncover the temporal changes in the runoff losses of the predominant nutrient proxies (total N and total P) from a typical rice-planting area (120 ha) in the Yangtze river delta region of China. Results: A high total N concentration in the rice-planting area was observed during the rice-planting season; however, significant fluctuation in the total P was evident throughout the year. The Δtotal N (drainage minus stream) parameter showed negative values with a mean of −0.25 mg L−1, while Δtotal P showed positive values with a mean of 0.06 mg L−1. The annual average N and P runoff from paddy field were 11.6 kg ha−1 and 1.5 kg ha−1, respectively. The window phases for the total N loss were mainly concentrated in the rice-growing season. However, the window phase for the total P loss was more dispersive throughout the year. No apparent relationships were found between rainfall and N and P concentrations by self-organizing map analysis. Conclusions: The high-resolution monitoring, in this study, suggested that nutrient loss loading rather than nutrient concentration was strongly related to surface runoff Therefore, fertilization before high-intensity rainfall should be avoided to mitigate the nutrient runoff losses and maintain the rice wetland eco-function. [ABSTRACT FROM AUTHOR]

Background: Tyrosine kinase inhibitors (TKIs) can significantly prolong overall survival for patients with advanced non‐small cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR)‐mutation or anaplastic lymphoma kinase (ALK)‐rearrangement. However, the real‐world evaluation status of ALK/EGFR in China remains unclear. Methods: We conducted a prospective study including 1134 patients with cytologically or histologically confirmed advanced NSCLC (stage IIIb–IV) at 12 Chinese hospitals. Results: The most common evaluation methods were amplification‐refractory mutation system for EGFR status and immunohistochemistry targeting D5F3 for ALK status. Among patients with non‐squamous, the EGFR mutation rate was 44.1% and the ALK rearrangement rate was 10.0%. Among patients with squamous cell carcinoma, the EGFR mutation rate was 8.3% and the ALK rearrangement rate was 3.7%. Among all patients, gender (HR = 1.7, 95%CI = 1.2–2.4, P = 0.006), smoking history (HR = 1.8, 95%CI = 1.3–2.7, P = 0.001), histology (HR = 5.0, 95%CI = 2.4–10.1, P < 0.001), and brain metastases (HR = 1.5, 95%CI = 1.1–2.2, P = 0.017) were independent predictors of EGFR mutation, while age (HR = 2.6, 95%CI = 1.7–4.1, P < 0.001) was an independent predictor of ALK rearrangement. The median time from tumor diagnosis to EGFR or ALK status confirmation was 7 and 5 days, respectively. Targeted therapy rate was 73.8% in EGFR‐positive patients and 51.4% in ALK‐positive patients. There was a negative correlation between the first‐line targeted therapy rate and the EGFR mutation detection period (r = −0.152, P = 0.02), while no significant correlation among patients with ALK rearrangement (r = −0.179, P = 0.076). Conclusion: Squamous NSCLC patients should also be routinely tested to determine their EGFR/ALK statuses. The first‐line targeted therapy rate remains low in Chinese patients with NSCLC. [ABSTRACT FROM AUTHOR]

Background: Thymoma and thymic carcinoma are rare thymic epithelial tumors. We investigated the efficacy of first‐line gemcitabine and cisplatin (GP) chemotherapy versus gemcitabine and cisplatin chemotherapy combined with the anti‐angiogenic drug endostar (GP + E) in advanced thymoma and thymic carcinoma. Methods: The records of 45 patients with invasive metastatic thymomas or thymic carcinomas treated with GP as first‐line therapy between August 2008 and July 2017 at the Department of Respiratory Medicine, Peking University Cancer Hospital and Institute were retrospectively reviewed. Results: Eighteen patients (75%) in the GP + E group achieved a partial response and six (25%) had stable disease. In GP only group, nine (42.8%) patients achieved a partial response, 11 (52.4%) had stable disease, and one (4.8%) had progressive disease. The GP + E group had a significantly higher overall response rate (75% vs. 42.9%; P = 0.028), and median progression‐free survival (PFS) and overall survival (OS) of 19 and 76 months, respectively. In the GP only group, median PFS and OS were 16 and 29 months, respectively. PFS and OS were not significantly different between the groups. Conclusions: GP has moderate efficacy and could represent a suitable first‐line therapy for thymic carcinoma and thymoma. Chemotherapy combined with endostar could improve the overall response rate, but did not prolong PFS or OS. [ABSTRACT FROM AUTHOR]

Background The programmed cell death-1/programmed cell death-1 ligand ( PD-1/ PD- L1) pathway plays a crucial role in tumor evasion. This study evaluated the association between circulating PD-L1 expression and clinical characteristics in patients with advanced non-small cell lung cancer ( NSCLC). Methods A total of 109 advanced NSCLC and 65 healthy patients from the Beijng Cancer Hospital were enrolled in the study. Circulating PD- L1 expression was tested by enzyme-linked immunosorbent assay. The associations between the level of PD- L1 expression and clinicopathologic features and prognosis were statistically analyzed. Results The expression of PD- L1 in advanced NSCLC patients was significantly upregulated compared with the healthy control ( P < 0.001). The expression of PD-L1 was significantly correlated with abdominal organ metastasis ( P = 0.004). A high PD- L1 expression had a worse prognosis than a low expression in patients (18.7 vs. 26.8 month, P < 0.001). Conclusions PD- L1 was elevated in advanced NSCLC patients and may play an important role in tumor immune evasion and patient prognosis. [ABSTRACT FROM AUTHOR]

Avian leukosis virus (ALV) is an enveloped retrovirus with a single-stranded RNA genome, belonging to the genus Alpharetrovirus within the family Retroviridae. The disease (Avian leukosis, AL) caused by ALV is mainly characterized by tumor development and immunosuppression in chickens, which increases susceptibility to other pathogens and leads to significant economic losses in the Chinese poultry industry. The government and poultry industry have made lots of efforts to eradicate ALV, but the threat of which remains not vanished. This review provides a summary of the updated understanding of ALV in China, which mainly focuses on genetic and molecular biology, epidemiology, and diagnostic methods. Additionally, promising antiviral agents and ALV eradication strategies performed in China are also included. [ABSTRACT FROM AUTHOR]

Increasing nitrogen oxides (NO x ) emissions over the fast developing regions have been of great concern due to their critical associations with the aggravated haze and climate change. However, little geographically specific data exists for estimating spatio-temporal trends of NO x emissions. In order to quantify the spatial and temporal variations of NO x emissions, a spatially explicit approach based on the continuous satellite observations of artificial nighttime stable lights (NSLs) from the Defense Meteorological Satellite Program/Operational Linescan System (DMSP/OLS) was developed to estimate NO x emissions from the largest emission source of fossil fuel combustion. The NSL based model was established with three types of data including satellite data of nighttime stable lights, geographical data of administrative boundaries, and provincial energy consumptions in China, where a significant growth of NO x emission has experienced during three policy stages corresponding to the 9 th –11 th Five-Year Plan (FYP, 1995–2010). The estimated national NO x emissions increased by 8.2% per year during the study period, and the total annual NO x emissions in China estimated by the NSL-based model were approximately 4.1%–13.8% higher than the previous estimates. The spatio-temporal variations of NO x emissions at city scale were then evaluated by the Moran's I indices. The global Moran's I indices for measuring spatial agglomerations of China's NO x emission increased by 50.7% during 1995–2010. Although the inland cities have shown larger contribution to the emission growth than the more developed coastal cities since 2005, the High–High clusters of NO x emission located in Beijing-Tianjin-Hebei regions, the Yangtze River Delta, and the Pearl River Delta should still be the major focus of NO x mitigation. Our results indicate that the readily available DMSP/OLS nighttime stable lights based model could be an easily accessible and effective tool for achieving strategic decision making toward NO x reduction. [ABSTRACT FROM AUTHOR]

Chemokines direct cell migration in development and immune defense, and bridge between innate and adaptive immune responses. The chemokine gene family has been rapidly evolving and has undergone species/lineage-specific expansion. Mammals possess inflammatory CXC chemokines CXCL1-8/15 and CXCL9-11 sub-groups, and homeostatic CXCL12-14, 16–17. Orthologues of mammalian CXCL12-14, three chemokines related to CXCL1-8/15 (CXCL8_L1-3), two chemokines related to CXC9-11 (CXCL11_L1-2), and five fish-specific chemokines (CXCL_F1-5) have been described in teleosts. In this study, we reported three novel CXC chemokines in Asian swamp eel Monopterus albus, a commercially important freshwater fish species in China. Two of them belong to the fish-specific CXCL_F2 group, named CXCL_F2a/b, that share 89.5% amino acid identity. The other (CXCL11_L3) belongs to a third CXCL11_L related to the mammalian CXCL9-11 subfamily found only in percomorph fish species, and is the only CXCL9-11 related molecules in this lineage. Mammalian CXCL9-11 attract Th1 cells, and block the migration of Th2 cells in an immune response. This study suggests that all major lineages of teleosts have a CXCL9-11 related chemokine that will aid future functional investigation of CXCL11_L in fish. Cxcl_f2a is highly expressed constitutively in the skin of swamp eels that may attract immune cells to protect the skin in the absence of scales. Cxcl11_l3 and cxcl_f2b are highly expressed in immune tissues/organs and are up-regulated by the viral mimic poly I:C, but not bacterial infection in vivo , suggesting their role in anti-viral defense. The two cxcl_f2 paralogues are differentially expressed and modulated, indicating sub- and/or neo-functionalization. • CXCL11_L3 represents a third CXCL9-11 related chemokine in percomorph fish species. • Cxcl_f2a and cxcl_f2b are differentially expressed and modulated in vivo. • Cxcl11_l3 and cxcl_f2b are induced by poly I:C suggesting a role in anti-viral defence. [ABSTRACT FROM AUTHOR]

Eutrophication can stimulate the emissions of volatile sulfur compounds (VSCs) accompanied by variations in environmental variables in lakes. However, the effects of eutrophication on VSC emissions from lake sediments as well as the underlying mechanisms remain unclear. In this study, depth gradient sediments at different eutrophication levels and seasons were collected from Lake Taihu to investigate the response of sulfur biotransformation in the sediments to eutrophication based on the analysis of environmental variables, microbial activity, abundance and community structure. H 2 S and CS 2 were the main VSCs produced from the lake sediments, with the production rates of 2.3-7.9 and 1.2-3.9 ng g -1  h -1 in August, respectively, which were higher than those in March, mainly due to the increasing activity and abundance of sulfate-reducing bacteria (SRB) at high temperatures. The VSC production rates from the sediments increased with lake eutrophication level. Higher VSC production rates were detected in surface sediments in eutrophic regions but in deep sediments in oligotrophic regions. Sulfuricurvum, Thiobacillus and Sulfuricella were the main sulfur-oxidizing bacteria (SOB) in the sediments, while Desulfatiglans and Desulfobacca were the predominant SRB. Organic matter, Fe 3+ , NO 3 - -N and total sulfur had significant influences on the microbial communities in the sediments. Partial least squares path modelling showed that the trophic level index could stimulate VSC emissions from lake sediments by influencing the activities and abundances of SOB and SRB. These findings indicated that sediments contributed substantially to VSC emissions from eutrophic lakes, especially surface sediments, and sediment dredging might be an effective way to mitigate VSC emissions from eutrophic lakes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Eutrophic lakes are hot spots of CH 4 and volatile sulfur compound (VSC) emissions, especially during algal blooms and decay. However, the response of CH 4 and VSC emissions to lake eutrophication and algae growth as well as the underlying mechanisms remain unclear. In this study, the emissions of CH 4 and VSCs from four regions of Lake Taihu with different eutrophic levels were investigated in four months (i.e., March, May, August and December). The CH 4 emissions ranged from 20.4 to 126.9 mg m -2 d -1 in the investigated sites and increased with eutrophic levels and temperature. H 2 S and CS 2 were the dominant volatile sulfur compounds (VSCs) emitted from the lake. The CH 4 oxidation potential of water ranged from 2.1 to 14.9 μg h -1 L -1 , which had positive correlations with trophic level index and the environmental variables except for the NH 4 + -N concentration. Eutrophic levels could increase the abundances of bacteria and methanotrophs in lake water. α-Proteobacteria methanotroph Methylocystis was more abundant than γ-Proteobacteria methanotrophs in March and May, while the latter was more abundant in August and November. The relative abundance of Cyanobacteria, including Microcystis, A. granulata var. angustissima and Cyanobium had significantly positive correlations with temperature, turbidity, SO 4 2- -S, and total sulfur. Partial least squares path modelling revealed that the algal growth could promote VSC emissions, which had a positive correlation with CH 4 oxidation potential, likely due to the positive correlation between the CH 4 and VSC emissions from lakes. These findings indicate that water eutrophication and algae growth could increase the emissions of CH 4 and VSCs from lakes. Controlling algae growth might be an effective way to mitigate the emissions of CH 4 and VSCs from freshwater lakes., (Copyright © 2022. Published by Elsevier Ltd.)

Ammonia oxidation is performed by both ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). However, the current knowledge of the distribution, diversity, and relative abundance of these two microbial groups in freshwater sediments is insufficient. We examined the spatial distribution and analyzed the possible factors leading to the niche segregation of AOA and AOB in the sediments of the Qiantang River, using clone library construction and quantitative PCR for both archaeal and bacterial amoA genes. pH and NH4(+)-N content had a significant effect on AOA abundance and AOA operational taxonomy unit (OTU) numbers. pH and organic carbon content influenced the ratio of AOA/AOB OTU numbers significantly. The influence of these factors showed an obvious spatial trend along the Qiantang River. This result suggested that AOA may contribute more than AOB to the upstream reaches of the Qiantang River, where the pH is lower and the organic carbon and NH4(+)-N contents are higher, but AOB were the principal driver of nitrification downstream, where the opposite environmental conditions were present.

As the largest bread basket in Zhejiang Province, Hangjiahu area is facing more and more serious water pollution, while the N loss loading by runoff from the paddy field during its submersed period is the main cause of the pollution. Through field experiment and fixed spot observation, the model of precipitation - runoff in Yangtze delta was testified, and the results showed that the precipitation - runoff model from HE Baogen was basically accorded with the fact after considering the impact of field overflow mouth, and the error was between - 19. 9% and + 18. 0%. The model of N concentration with precipitation - runoff in paddy field during submersed period was brought forward, with the R value being 0. 948. These two models consisted of the model of N loss loading by runoff from paddy field during submersed period. Based on this model as well as the past 30 years data of fertilization and precipitation, 1: 250,000 topography map, land use map, and water system map, the N loss loading and its distribution were estimated by using GIS method, and the results showed that the N loss loading was different from place to place, with an average of 35.26 kg N x hm(-2), and accounting for 12. 69% of the applied N. The N loss loading in Anji and Yuhang with obviously more precipitation was higher than that in other places, while Haining also had a serious N loss problem because of the huge amount of applied N.