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BACKGROUND: Range of Motion (ROM) and passive torque have been widely used to evaluate flexibility stretching protocols, but little attention has been given to the reliability of these measurements. OBJECTIVE: To verify the interday reliability of isokinetic dynamometer for assessing ROM measures based on discomfort perception (initial and maximum), static and dynamic measures of passive torque during a leg extension movement on a young adult sample. METHODS: Twenty students (13 men; 7 women) aged between 18 and 30 years attended the laboratory to perform the test and retest with 2-7 days interval. The evaluations included: Initial Discomfort Angle (IDA), Passive dynamic torque (Stiffness), Passive static torque (Viscoelastic Stress Relaxation-VSR) and Maximal Discomfort Angle (MDA). Reliability was tested by the Intraclass Correlation Coefficient (ICC), one-sample t-test and Bland-Altman charts. RESULTS: Stiffness (ICC = 0.93), IDA (ICC = 0.90) and MDA (ICC = 0.87) showed excellent reliability values, while VSR had moderate ones (ICC = 0.59). Bland-Altman plots indicated bias close or equal to zero. The one-sample t-test for all measures reinforced the absence of a systematic error. CONCLUSIONS: Dynamic and static stiffness measures and ROM based on the subject's discomfort perception may be applied to relevant clinical issues. [ABSTRACT FROM AUTHOR]