1. A Metagenomic Investigation of the Viruses Associated with Shiraz Disease in Australia.
- Author
-
Wu, Qi, Habili, Nuredin, Kinoti, Wycliff M., Tyerman, Stephen D., Rinaldo, Amy, Zheng, Linda, and Constable, Fiona E.
- Subjects
- *
GRAPEVINE leafroll virus , *SYRAH , *METAGENOMICS , *GRAPE diseases & pests , *GRAPES , *NUCLEOTIDE sequencing - Abstract
Shiraz disease (SD) is an economically important virus-associated disease that can significantly reduce yield in sensitive grapevine varieties and has so far only been reported in South Africa and Australia. In this study, RT-PCR and metagenomic high-throughput sequencing was used to study the virome of symptomatic and asymptomatic grapevines within vineyards affected by SD and located in South Australia. Results showed that grapevine virus A (GVA) phylogroup II variants were strongly associated with SD symptoms in Shiraz grapevines that also had mixed infections of viruses including combinations of grapevine leafroll-associated virus 3 (GLRaV-3) and grapevine leafroll-associated virus 4 strains 5, 6 and 9 (GLRaV-4/5, GLRaV-4/6, GLRaV-4/9). GVA phylogroup III variants, on the other hand, were present in both symptomatic and asymptomatic grapevines, suggesting no or decreased virulence of these strains. Similarly, only GVA phylogroup I variants were found in heritage Shiraz grapevines affected by mild leafroll disease, along with GLRaV-1, suggesting this phylogroup may not be associated with SD. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF