1. Draw-fill batch culture mode for production of xylanases by Cellulomonas flavigena on sugar cane bagasse.
- Author
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Vega-Estrada J, Flores-Cotera LB, Santiago A, Magaña-Plaza I, and Montes-Horcasitas C
- Subjects
- Actinomycetales genetics, Actinomycetales growth & development, Biomass, Biotechnology instrumentation, Cellulose, Industrial Microbiology, Substrate Specificity, Xylan Endo-1,3-beta-Xylosidase, Actinomycetales enzymology, Biotechnology methods, Fermentation, Xylosidases biosynthesis
- Abstract
Draw-fill culture was evaluated as a method for xylanase production by Cellulomonas flavigena on sugar cane bagasse. Specific xylanase activity and volumetric xylanase activities were measured by harvesting 50%, 55%, 60% and 70% of fermented broth at the end of each subculture. Maximum specific (64 IU mg(-1) protein) and volumetric (166 IU ml(-1)) xylanase activities were obtained by harvesting 50-55% of broth. Values were 3.4 and 3.8 times greater than those obtained in batch cultures carried out under the same conditions. Enzyme productivity of 4.2 IU ml(-1) h(-1) was significantly greater than that obtained in continuous cultures (2.4 IU ml(-1) h(-1)) (P<0.05).
- Published
- 2002
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