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Draw-fill batch culture mode for production of xylanases by Cellulomonas flavigena on sugar cane bagasse.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2002 Mar; Vol. 58 (4), pp. 435-8. Date of Electronic Publication: 2002 Jan 19. - Publication Year :
- 2002
-
Abstract
- Draw-fill culture was evaluated as a method for xylanase production by Cellulomonas flavigena on sugar cane bagasse. Specific xylanase activity and volumetric xylanase activities were measured by harvesting 50%, 55%, 60% and 70% of fermented broth at the end of each subculture. Maximum specific (64 IU mg(-1) protein) and volumetric (166 IU ml(-1)) xylanase activities were obtained by harvesting 50-55% of broth. Values were 3.4 and 3.8 times greater than those obtained in batch cultures carried out under the same conditions. Enzyme productivity of 4.2 IU ml(-1) h(-1) was significantly greater than that obtained in continuous cultures (2.4 IU ml(-1) h(-1)) (P<0.05).
Details
- Language :
- English
- ISSN :
- 0175-7598
- Volume :
- 58
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 11954788
- Full Text :
- https://doi.org/10.1007/s00253-001-0908-1