1. Rare subtypes of BK virus are viable and frequently detected in renal transplant recipients with BK virus-associated nephropathy
- Author
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Jennifer Trofe-Clark, Jennifer Gordon, E. Steve Woodle, Jessica Otte, Prabir Roy Chaudhury, Pasquale Ferrante, Sara Tremolada, Kamel Khalili, Selma Akan, and Martyn K. White
- Subjects
viruses ,Population ,Urine ,Biology ,medicine.disease_cause ,Cell Line ,Nephropathy ,Viral Proteins ,BK virus ,Virology ,Chlorocebus aethiops ,medicine ,Animals ,Humans ,Polyomavirus-associated nephropathy ,Cloning, Molecular ,education ,Kidney transplant ,Vero Cells ,Kidney transplantation ,BKV ,Polyomavirus Infections ,Kidney ,education.field_of_study ,virus diseases ,medicine.disease ,Kidney Transplantation ,Subtyping ,Tumor Virus Infections ,medicine.anatomical_structure ,Amino Acid Substitution ,Viral replication ,COS Cells ,Immunology ,Vero cell ,Kidney Diseases - Abstract
BK virus-associated nephropathy (BKVN) occurs in up to 5% of kidney transplants and is a significant cause of graft loss. Four major subtypes of BKV have been described, with the vast majority of individuals persistently infected with BKV Type I (>80% of the population). Sequencing of BKV isolates subcloned from BKVN patients revealed a high percentage of variants in the urine (40%) in the VP1 subtyping region. In vitro analysis of several viral variants revealed that all variants recovered from the urine of BKVN patients produced infectious viral particles and were replication competent in cell culture while some of the variants induced cytopathic changes in infected cells when compared to the major BKV subtype, VP1 Type I. These results suggest that rare BKV VP1 variants are more frequently associated with disease and that some variants could be more cytopathic than others in renal transplant recipients.
- Published
- 2010
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