1. [Human recombinant antibodies to variola virus].
- Author
-
Tikunova NV, Bovshik EI, Iun TE, Zhirakovskaia EV, Morozova VV, Batanova TA, Gus'kov AA, Sokunova EB, Il'ichev AA, and Sandakhchiev LS
- Subjects
- Amino Acid Sequence, Antibodies, Viral biosynthesis, Antibodies, Viral genetics, Combinatorial Chemistry Techniques, Cross Reactions, Humans, Immunoglobulin Variable Region biosynthesis, Immunoglobulin Variable Region genetics, Immunoglobulin Variable Region immunology, Peptide Library, Recombinant Proteins genetics, Recombinant Proteins immunology, Antibodies, Viral immunology, Variola virus immunology
- Abstract
Eight specific antibodies to live variola virus (VV), Ind-3a strain, and 7 antibodies to VV, Butler strain, were selected from the synthetic combinatorial phage display library on single-chain (scFv) human antibodies. Indirect solid-phase enzyme immunoassay showed the ability of these antibodies to bind the VV strains Ind-3a, Butler, Brazil-131, Kuw-5, and Congo-2. Moreover, earlier selected human scFv antibodies were also tested in the reaction of binding to the above VV strains. The experiments could reveal the antibodies that bound alastrim strains more effectively that did other VV strains. The nucleotide sequences encoding for the selected scFv antibodies were determined.
- Published
- 2005