1. An injectable active hydrogel based on BMSC-derived extracellular matrix for cartilage regeneration enhancement.
- Author
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Wei B, Xu Y, Tang C, Liu NQ, Li X, Yao Q, and Wang L
- Subjects
- Animals, Cell Proliferation, Cell Differentiation, Rabbits, Cell Adhesion, Humans, Injections, Extracellular Matrix chemistry, Extracellular Matrix metabolism, Regeneration, Mesenchymal Stem Cells cytology, Cartilage, Articular physiology, Cartilage, Articular injuries, Chondrogenesis, Hydrogels chemistry, Tissue Scaffolds chemistry, Chondrocytes transplantation, Tissue Engineering methods
- Abstract
Articular cartilage injury impairs joint function and necessitates orthopedic intervention to restore the structure and function of the cartilage. Extracellular matrix (ECM) scaffolds derived from bone marrow mesenchymal stem cells (BMSCs) can effectively promote cell adhesion, proliferation, and chondrogenesis. However, pre-shaped ECM scaffolds have limited applicability due to their poor fit with the irregular surface of most articular cartilage defects. In this study, we fabricated an injectable active ECM hydrogel from autologous BMSCs-derived ECM by freeze-drying, liquid nitrogen milling, and enzymatic digestion. Moreover, our in vitro and in vivo results demonstrated that the prepared hydrogel enhanced chondrocyte adhesion and proliferation, chondrogenesis, cartilage regeneration, and integration with host tissue, respectively. These findings indicate that active ECM components can provide trophic support for cell proliferation and differentiation, restoring the structure and function of damaged cartilage., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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