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Start Over Author "F. BONDIOLI" Topic tissue scaffolds
3 results on '"F. BONDIOLI"'

1. Highly porous PHB-based bioactive scaffolds for bone tissue engineering by in situ synthesis of hydroxyapatite.

Author

Degli Esposti M, Chiellini F, Bondioli F, Morselli D, and Fabbri P

Subjects
Alkaline Phosphatase metabolism, Animals, Bone and Bones drug effects, Cell Line, Cell Proliferation drug effects, Collagen biosynthesis, Durapatite chemistry, Hydroxybutyrates chemistry, Mice, Molecular Weight, Polyesters chemistry, Porosity, Temperature, Thermogravimetry, X-Ray Diffraction, Biocompatible Materials pharmacology, Bone and Bones physiology, Durapatite chemical synthesis, Hydroxybutyrates pharmacology, Polyesters pharmacology, Tissue Engineering methods, Tissue Scaffolds chemistry
Abstract

In this study bioactive and bioresorbable porous scaffolds for bone tissue regeneration, based on poly(3-hydroxybutyrate) (PHB), are presented. The porous structure is obtained by thermally induced phase separation (TIPS) technique, whereas the osteoinductivity and osteoconductivity are enhanced through the incorporation of hydroxyapatite (HA). The HA particles are generated in PHB using an innovative filler in situ synthesis, and the properties of the composite scaffolds are then compared to scaffolds obtained by conventional mechanical dispersion of ex situ synthesized HA particles. The in situ synthesis leads to composite materials with improved porosity, even at high filler content, without any degradation of the polymeric matrix as confirmed by GPC and DSC measurements. On the contrary, the samples prepared by ex situ method show a suppressed porosity by increasing the inorganic filler content, therefore limiting the amount of HA that can be loaded in PHB and the resulting bioactivity. The possibility to use PHB/HA porous composites as scaffolds for bone tissue regeneration, is assessed by preliminary cell viability in vitro studies. In particular, it is observed that the composites are fully cytocompatible and able to sustain MC3T3-E1 mouse pre-osteoblast cells adhesion and proliferation. Investigations on cell morphology reveal, for all PHB/HA scaffolds, the presence of differentiated cells with a predominance of osteocyte-like morphology, which are not observed for neat PHB scaffolds. Moreover, the MC3T3-E1 cells differentiation towards osteoblastic phenotype is further supported by the evaluation of the early osteogenic markers. In particular, samples loaded with HA in situ synthesized showed the highest ALP production and typical morphology of the terminal differentiation stages of osteoblasts., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Published
2019
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2. Porous scaffolds of polycaprolactone reinforced with in situ generated hydroxyapatite for bone tissue engineering.

Author

Fabbri P, Bondioli F, Messori M, Bartoli C, Dinucci D, and Chiellini F

Subjects
Animals, BALB 3T3 Cells, Biomechanical Phenomena, Bone and Bones cytology, Bone and Bones drug effects, Cell Adhesion drug effects, Cell Adhesion physiology, Cell Proliferation drug effects, Cells, Cultured, Durapatite chemical synthesis, Materials Testing, Mice, Microscopy, Electron, Transmission, Phase Transition, Porosity, Tissue Engineering methods, Water pharmacology, Bone and Bones physiology, Durapatite chemistry, Polyesters chemistry, Tissue Scaffolds chemistry
Abstract

Polycaprolactone/hydroxyapatite (PCL/HA) composites were prepared by in situ generation of HA in the polymer solution starting from the precursors calcium nitrate tetrahydrate and ammonium dihydrogen phosphate via sol-gel process. Highly interconnected porosity was achieved by means of the salt-leaching technique using a mixture of sodium chloride and sodium bicarbonate as porogens. Structure and morphology of the PCL/HA composites were investigated by scanning electron microscopy, and mechanical properties were determined by means of tensile and compression tests. The possibility to employ the developed composites as scaffolds for bone tissue regeneration was assessed by cytotoxicity test of the PCL/HA composites extracts and cell adhesion and proliferation in vitro studies.

Published
2010
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3. Porous scaffolds of polycaprolactone reinforced with in situ generated hydroxyapatite for bone tissue engineering

Author

Dinuccio Dinucci, Cristina Bartoli, Federica Chiellini, Massimo Messori, Federica Bondioli, Paola Fabbri, P. Fabbri, F. Bondioli, M. Messori, C. Bartoli, D. Dinucci, and F. Chiellini

Subjects
Materials science, BALB 3T3 Cells, Sodium, Polyesters, Biomedical Engineering, Biophysics, chemistry.chemical_element, Bioengineering, Bone tissue, Bone and Bones, Phase Transition, Biomaterials, Scaffold, chemistry.chemical_compound, Mice, Tissue engineering, Microscopy, Electron, Transmission, polycaprolactone, Ultimate tensile strength, Materials Testing, medicine, Cell Adhesion, Animals, Composite material, Cells, Cultured, Cell Proliferation, Tissue Scaffolds, technology, industry, and agriculture, Biomaterial, Water, hydroxyapatite, POLYCAPROLACTONE, TISSUE ENGINEERING, Ammonium dihydrogen phosphate, HYDROXYAPATITE, Biomechanical Phenomena, Polyester, medicine.anatomical_structure, Durapatite, chemistry, Chemical engineering, scaffolds, tissue engineering, Polycaprolactone, Porosity
Abstract

Polycaprolactone/hydroxyapatite (PCL/HA) composites were prepared by in situ generation of HA in the polymer solution starting from the precursors calcium nitrate tetrahydrate and ammonium dihydrogen phosphate via sol–gel process. Highly interconnected porosity was achieved by means of the salt-leaching technique using a mixture of sodium chloride and sodium bicarbonate as porogens. Structure and morphology of the PCL/HA composites were investigated by scanning electron microscopy, and mechanical properties were determined by means of tensile and compression tests. The possibility to employ the developed composites as scaffolds for bone tissue regeneration was assessed by cytotoxicity test of the PCL/HA composites extracts and cell adhesion and pro- liferation in vitro studies.

Published
2010

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