11 results on '"Ma, Zhongjun"'
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2. Complete genome sequence of Streptomyces sp. HNA39, a new cyclizidine producer isolated from a South China Sea sediment.
- Author
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Li S, Jiang YJ, Ma Z, and Wang N
- Subjects
- Base Sequence, Chromosome Mapping, China, Multigene Family, Streptomyces genetics
- Abstract
Streptomyces sp. HNA39 is a promising candidate for the production of antineoplastic metabolites screened from a collection of 448 actinomycetes derived from coastal sediments. The complete genome sequence of HNA39 comprises a 7,351,753-bp linear chromosome with a GC content of 71.94%. Whole genome analysis reveals the presence of 29 putative biosynthetic gene clusters (BGCs) encoding secondary metabolites. Among them, a type I PKS BGC shows an 82% similarity with the cyclizidine (CLD) BGC identified from Streptomyces NCIB 11649. LC-MS profiles further supported the production of new CLD congeners. Bafilomycins were also found produced in abundance, corresponding to another type I PKS BGC highly homologous to that of bafilomycin B1 from S. lohii. CLDs are indolizidine alkaloids consisting a fused five- and six-membered ring system with an intriguing cyclopropane terminal linked by a trans-dienic chain. The cyclization mechanism of the cylopropyl ring, one of its pharmacophores, is still unknown. Genome sequencing of the new CLD producer and subsequent comparative analysis of their gene clusters would further our understanding of the chemistry behind cyclopropane formation., Competing Interests: Declaration of Competing Interest The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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3. Suncheonosides E-M and Benzothioate Derivatives from the Marine-Derived Streptomyces sp. ZSN77.
- Author
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Jiang M, Zhang Y, Zhang Y, Ma Z, and Wang J
- Subjects
- HCT116 Cells, Humans, Magnetic Resonance Spectroscopy methods, Molecular Structure, PC-3 Cells, Streptomyces chemistry
- Abstract
Thirteen new compounds, including suncheonosides E-M ( 1 - 9 ), four benzothioate derivatives ( 10 - 13 ), and one known compound ( 14 ), were identified from the marine-derived Streptomyces sp. ZSN77. Suncheonosides E-M incorporate β-d-glucose, while the reported suncheonosides (A-D) incorporate only l-rhamnose. All of the structures were determined by extensive analysis of NMR spectroscopic and HRESIMS data. Bioactivity evaluation of these compounds showed that 6 had significant activity against PC3 cells with an IC
50 value of 4.1 ± 0.1 μM, while compounds 12 and 14 exhibited cytotoxicity against HCT116 cells with IC50 values of 7.3 ± 0.4 and 3.9 ± 0.3 μM, respectively. In addition, compounds 1 , 2 , 6 , 10 , and 14 displayed potent in vivo anti-inflammatory efficacy with inhibition of NO production in a dose-dependent manner.- Published
- 2022
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4. Aminoacyl sulfonamide assembly in SB-203208 biosynthesis.
- Author
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Hu Z, Awakawa T, Ma Z, and Abe I
- Subjects
- Alkaloids metabolism, Aminoacyltransferases genetics, Isoleucine metabolism, Pyridines metabolism, Streptomyces genetics, Sulfur Compounds metabolism, Aminoacyltransferases metabolism, Biosynthetic Pathways genetics, Indenes metabolism, Multigene Family, Streptomyces metabolism, Sulfonamides metabolism
- Abstract
Sulfonamide is present in many important drugs, due to its unique chemical and biological properties. In contrast, naturally occurring sulfonamides are rare, and their biosynthetic knowledge are scarce. Here we identify the biosynthetic gene cluster of sulfonamide antibiotics, altemicidin, SB-203207, and SB-203208, from Streptomyces sp. NCIMB40513. The heterologous gene expression and biochemical analyses reveal unique aminoacyl transfer reactions, including the tRNA synthetase-like enzyme SbzA-catalyzed L-isoleucine transfer and the GNAT enzyme SbzC-catalyzed β-methylphenylalanine transfer. Furthermore, we elucidate the biogenesis of 2-sulfamoylacetic acid from L-cysteine, by the collaboration of the cupin dioxygenase SbzM and the aldehyde dehydrogenase SbzJ. Remarkably, SbzM catalyzes the two-step oxidation and decarboxylation of L-cysteine, and the subsequent intramolecular amino group rearrangement leads to N-S bond formation. This detailed analysis of the aminoacyl sulfonamide antibiotics biosynthetic machineries paves the way toward investigations of sulfonamide biosynthesis and its engineering.
- Published
- 2019
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5. Bisamides and rhamnosides from mangrove actinomycete Streptomyces sp. SZ-A15.
- Author
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Ma Q, Ding W, Chen Z, and Ma Z
- Subjects
- Amides pharmacology, Antineoplastic Agents pharmacology, Cell Cycle Proteins, Glycosides pharmacology, Humans, Magnetic Resonance Spectroscopy, Molecular Structure, Spectrophotometry, Ultraviolet, Streptomyces metabolism, Wetlands, Amides chemistry, Antineoplastic Agents chemistry, Glycosides chemistry, Nuclear Proteins antagonists & inhibitors, Streptomyces chemistry, Transcription Factors antagonists & inhibitors
- Abstract
Four new bisamides 1-4, and two new rhamnosides (5, 6), along with four known compounds 7-10, were isolated from a scale culture of the mangrove-derived actinomycete Streptomyces sp. SZ-A15. All structures were determined through analysis of the UV, IR, HRESIMS, 1D and 2D NMR spectra as well as by comparison with literature data. BRD4 inhibition of all isolated compounds was evaluated. As for the ability to inhibit protein BRD4, compound 9 exhibited moderate activity with the value of 78.4 ± 2.2% at 10 μM.
- Published
- 2018
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6. Induced production of cytochalasans in co-culture of marine fungus Aspergillus flavipes and actinomycete Streptomyces sp.
- Author
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Yu L, Ding W, and Ma Z
- Subjects
- Coculture Techniques, Cytochalasins chemistry, Cytochalasins pharmacology, Magnetic Resonance Spectroscopy, Microscopy, Electron, Scanning, Aspergillus metabolism, Cytochalasins biosynthesis, Streptomyces metabolism
- Abstract
Abstarct Secondary metabolites profiles of co-culture of Aspergillus flavipes and Streptomyces sp. that isolated from the same habitat showed an induced production of a series of cytochalasans (five aspochalasins and rosellichalasin, determined by MS and NMR analysis). These cytochalasans were found to be produced by A. flavipes in LC-MS comparison analysis, and biological activity assays revealed that they were able to cause cytotoxic effects against Streptomyces sp. within a wide range of concentrations without causing any effect to the producer A. flavipes, which favoured the producer in competition. Further induction mechanism study applying membrane-separated culture and morphology study with scanning electron microscopy (SEM) suggested that the successful induction of active secondary metabolites required microbial physical contact.
- Published
- 2016
- Full Text
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7. Interactions in the Competitive Coexistence Process of Streptomyces sp. and Escherichia coli.
- Author
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Yu L, Hu Z, Hu Z, and Ma Z
- Subjects
- Culture Media chemistry, Escherichia coli metabolism, Escherichia coli physiology, Hydrogen-Ion Concentration, Indole Alkaloids analysis, Streptomyces metabolism, Streptomyces physiology, Antibiosis, Escherichia coli growth & development, Streptomyces growth & development
- Abstract
Competitive coexistence of different microorganism species is a fundamental ecological process in the evolution and maintenance of biodiversity. This work studied the interactions happened in the competitive coexistence process of actinomycete Streptomyces sp. and Escherichia coli from morphological and secondary metabolites perspective. We found three important interactions occurred in their successful coexistence process: medium pH was elevated, indole alkaloids with dual inhibiting effects were produced, and culture environment was spatially structured. For the weed-like superior competitor E. coli, its massive growth was suppressed by the elevated pH and the newly produced novel bisindole alkaloid hepchrome. For the inferior Streptomyces sp., its mycelium floated to the medium surface for further colonization, and the growth in liquid medium was inhibited by its self-produced alkaloids such as halichrome A, 1,1,1-Tris (3-indolyl) methane, vibrindole A, and hepchrome. The coexistence of E. coli and Streptomyces sp. was thereby achieved through reduction of spatial and energy resource overlapping and suppression of competition.
- Published
- 2015
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8. Production of bioactive tryptamine derivatives by co-culture of marine Streptomyces with Bacillus mycoides.
- Author
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Yu L, Hu Z, and Ma Z
- Subjects
- Bacillus growth & development, Coculture Techniques, Culture Media, Fermentation, Industrial Microbiology, Molecular Structure, Streptomyces growth & development, Thiazoles, Bacillus metabolism, Streptomyces metabolism, Tryptamines biosynthesis
- Abstract
Tryptamine derivatives such as tryptamine and bacillamides were strong algicidal compounds promising in controlling harmful algae blooms, but their bioactivity and application researches were hindered by extremely low natural production rates. This study found an induced production of algicidal tryptamine derivatives by co-culture of marine Streptomyces with Bacillus mycoides, and optimised the culture method through changing important factors such as medium nutrition content, culture mode and pH value. The final established co-culture method used only 5 g yeast extracts and 5 g glycerol in 1 L 75% sea water, but got a yield of 14.9 mg/L N-acetyltryptamine, 2.8 mg/L N-propanoyltryptamine, 3.0 mg/L bacillamide A, 13.7 mg/L bacillamide B and 9.6 mg/L bacillamide C, which were all undetectable under normal culture conditions.
- Published
- 2015
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9. <italic>Streptomyces nigra</italic> sp. nov. Is a Novel Actinobacterium Isolated From Mangrove Soil and Exerts a Potent Antitumor Activity <italic>in Vitro</italic>.
- Author
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Chen, Can, Ye, Yanghui, Wang, Ruijun, Zhang, Yinglao, Wu, Chen, Debnath, Sanjit C., Ma, Zhongjun, Wang, Jidong, and Wu, Min
- Subjects
STREPTOMYCES ,MANGROVE soils ,ANTINEOPLASTIC agents - Abstract
A new bacterial strain, designated 452
T , was isolated from the rhizosphere soil of the mangroveAvicennia marina in China. As determined, its cell wall peptidoglycan contained LL-diaminopimelic acid; MK-9(H8) and MK-9(H6) were the major isoprenoid quinones; and iso-C16:0 (31.3%), anteiso-C15:0 (16.9%), and iso-C15:0 (12.5%) were the major cellular fatty acids (>10.0%). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 452T formed a distinct lineage in the clade of the genusStreptomyces , and was closely related toS. coerulescens DSM 40146T (99.6% sequence identity),S. bellus DSM 40185T (99.5%), andS. coeruleorubidus DSM 41172T (99.3%). The DNA-DNA relatedness between strain 452T and these type strains ranged between 29.3 and 42.3%. Based on the phenotypic, chemotaxonomic, and phylogenetic features, the strain 452T is considered to represent a novel species of the genusStreptomyces , for which the nameStreptomyces nigra sp. nov. is proposed. The type strain is 452T (=KCTC 39960T = MCCC 1K03346T ). Further, strain 452T extracts exhibited a pronounced antitumor activity against human cancer cell lines A549, HCT-116, and HepG2, but not against normal human colon cells CCD-18Co. Active substances in the fermentation broth of strain 452T were isolated by bioassay-guided analysis, and then purified using a macroporous resin, silica gel, sephadex LX-20 column, and semi-preparative high-performance liquid chromatography (HPLC). Eight proline-containing diketopiperazines, namely, cyclo(Pro-Ala), cyclo(Pro-Gly), cyclo(Pro-Phe), cyclo(Pro-Met), cyclo(Pro-Val), cyclo(Pro-Leu), cyclo(Pro-Tyr), and cyclo(L-Leu-trans-4-hydroxy-L-Pro), were identified by electrospray ionization mass spectrometry (MS) and nuclear magnetic resonance (NMR). The compounds displayed different levels of cytotoxicity. The highest cytotoxicity was exhibited by cyclo(Pro-Ala) and cyclo(Pro-Met) against A549 cells, and cyclo(Phe-Pro) and cyclo(Pro-Ala) against HCT-116 cells, with average IC50 values equal to 18.5, 27.3, 32.3, and 47.6 μg/mL, respectively. The diversity of diketopiperazines and other chemicals produced by 452T was further investigated using gas chromatography (GC)-MS and liquid chromatography (LC)-MS. The analysis revealed 16 types of metabolites with antitumor activity and 16 other types of diketopiperazines. Hence, extracts of the newly identified strain may be used a starting material for the development of antitumor agents. [ABSTRACT FROM AUTHOR]- Published
- 2018
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10. Thioquinomycins A-D, novel naphthothiophenediones from the marine-derived Streptomyces sp. SS17F.
- Author
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Zhang, Dashan, Jiang, Yongjun, Li, Jiaqi, Ding, Wanjing, Chen, Zhe, and Ma, Zhongjun
- Subjects
- *
STREPTOMYCES , *TUNICAMYCIN , *STREPTOMYCETACEAE , *CHEMICALS , *SPECTROSCOPE , *NAPHTHALENE - Abstract
Abstract Thioquinomycins A-D (1–4), four novel naphthothiophenediones were isolated from the rice-based medium of the marine-derived Streptomyces sp. SS17F. Their structures were elucidated by spectroscopic methods and HRESIMS data. The absolute configurations of all the compounds were elucidated by X-ray diffraction analysis, ECD and Mosher's method combined with 19F-NMR. Compounds 1–4 showed moderate cytotoxicity against NCI-H1975 with IC 50 values from 17.5 to 50 μ M. In addition, compounds 1–4 also exhibited inhibitory activities against PKCα and ROCK2 protein kinase. Graphical abstract Image 1 [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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11. Strepolyketides A-C, three novel SEK15-derived polyketides from Streptomyces sp. HN2A53.
- Author
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Jiang, Yongjun, Huang, Yun, Chen, Shuang, Ji, Yuanyuan, Ding, Wanjing, and Ma, Zhongjun
- Subjects
- *
POLYKETIDES , *STREPTOMYCES , *INFLUENZA A virus , *NEURAMINIDASE , *DIMERS , *DATA analysis - Abstract
• Three novel SEK15-derived polyketides were identified from Streptomyces sp. HN2A53. • Compounds 2 – 3 were the unique dimers of SEK15. • Compounds 2 – 4 showed moderate inhibitory activities against influenza virus neuraminidase. Three novel SEK15-derived polyketides, strepolyketides A-C (1 – 3), and the known SEK15 (4) were identified from Streptomyces sp. HN2A53. Their structures were established by comprehensive 1D and 2D NMR spectroscopic analysis and HRESIMS data. Compounds 2 – 3 were the unique dimers of SEK15. Biological evaluation of these compounds showed that compounds 2 – 4 showed moderate inhibitory activity against influenza virus neuraminidase (NA) in vitro. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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