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Background: Kinesio tapes (KTs) are tapes used in physical therapy and athletics. We sought to evaluate if wearing KTs for extended periods of time increases exposure to antibiotic resistant opportunistic pathogens and/or effects normal human skin bacteria., Methods: The study consisted of 10 volunteers wearing 9 KTs on their arms for 5 consecutive days. Microorganisms were isolated from fragments collected on the second and fifth day then analyzed. Bacteria were identified using the BIOLOG system. Resistance to selected antibiotics was performed using E-Test. The effect of KTs on the growth of Staphylococcus aureus, Staphylococcus epidermidis, and Micrococcus luteus was evaluated in a diffusion-based assay., Results: We found that KTs accumulated environmental bacteria. Among 22 species 8 were opportunistic pathogens, and all of them exhibited resistance to at least one antibiotic. None of the tapes produced inhibition zones against S. aureus. One tape, Leukotape P, caused growth inhibition in non-pathogenic S. epidermidis and M. luteus. The adhesive material of the tapes inhibited the growth of all tested bacteria., Conclusions: These results indicate that KTs may increase the exposure to antibiotic resistant pathogens which can accumulate from the environment. Further, extended exposure could lead to changes in normal skin microbiota, potentially contributing to increased risks of skin infections., (Copyright © 2022 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.)

Tobacco leaf contains antibacterial secondary metabolite compounds, such as phenol, alkaloids, and essential oils. This study compares the potential antibacterial effects of Indonesian tobacco leaf extracted using the heat reflux method (producing an extract) and pyrolysis method (providing a bio-oil). The tobacco leaf extract was challenged against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. The bio-oil from the pyrolysis method showed inhibitory Kirby Bauer zones higher than those of the extract from heat reflux method, with the maximum results in the pyrolysis method indicating zones of 6.35 mm (S. aureus), 5.90 mm (E. faecalis), 3.97 mm (E. coli), and 5.025 mm (P. aeruginosa). Further study analyzed the effectiveness of the disc and well diffusion antibacterial test methods for measuring the antibacterial effect of bio-oils against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. The bio-oil used in the well diffusion test method showed the most significant antibacterial effectiveness. It showed the biggest inhibition zone, with a maximum of 11.65 mm and 8.90 mm for E. coli and P. aeruginosa. Our results showed Nicotiana tabacum var. Virginia Bio Oil from Ponorogo (Indonesia) is a strong potential antimicrobial, especially using well diffusion test. [ABSTRACT FROM AUTHOR]

Background: Human platelets are a rich reservoir of molecules that promote regenerative processes and microbicidal activity. This activity might be increased by concentration in platelet-rich plasma (PRP) products and modulated by the presence of leukocytes. Despite extensive use in clinical procedures, only few studies have investigated PRP's real microbicidal potential. Therefore, this study aimed at comparing the in vitro microbicidal activity of platelets and leukocyte-enriched PRP (L-PRP) to pure platelet-rich plasma (P-PRP) and the contribution of leukocytes to microbicidal properties. Antimicrobial effects of P- and L-PRP were tested against Escherichia Coli, Staphylococcus Aureus, Klebsiella Pneumoniae, Pseudomonas Aeruginosa and Enterococcus Faecalis. Furthermore, L-PRP was frozen (L-PRP cryo) to assess whether the preparation maintained in vitro characteristics. Microbicidal proteins released by the three preparations were also evaluated. Results: L-PRP, L-PRP cryo and P-PRP generally induced comparable bacterial growth inhibition for up to 4 h' incubation, range 1-4 log. MIP-1a, RANTES, GRO-a, IL-8, NAP-2, SDF-1a and IL-6 showed strong microbicidal potential. Conclusions: We found in vitro antibacterial activity of L-PRP and P-PRP and the possibility to cryopreserve L-PRP, without important changes to its effectiveness; similar microbicidal activity between preparations containing or not leukocytes; and the contribution of three new molecules (NAP-2, SDF-1a and IL-6). [ABSTRACT FROM AUTHOR]