1. Staphylococcus ursi sp. nov., a new member of the ' Staphylococcus intermedius group' isolated from healthy black bears.
- Author
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Perreten V, Kania SA, and Bemis D
- Subjects
- Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Fatty Acids chemistry, Genes, Bacterial, Nucleic Acid Hybridization, Peptidoglycan chemistry, Phospholipids chemistry, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Staphylococcus isolation & purification, Staphylococcus intermedius genetics, Tennessee, Vitamin K 2 analogs & derivatives, Vitamin K 2 chemistry, Phylogeny, Staphylococcus classification, Ursidae microbiology
- Abstract
Six Staphylococcus strains were isolated from healthy black bears ( Ursus americanus ) in the Great Smoky Mountains National Park, Tennessee, USA. Phylogenetic analysis based on complete genome, 16S rRNA, dnaJ , hsp60 , rpoB and sodA genes, and MALDI-TOF-MS main spectral profiles revealed that the strains belonged to one species and showed the closest relatedness to members of the ' Staphylococcus intermedius group' (SIG), which include Staphylococcus intermedius , Staphylococcus pseudintermedius, Staphylococcus delphini and Staphyloccoccus cornubiensis . The strains were positive in SIG-specific and negative in individual species-specific PCR assays for the nuc gene. The strains can be differentiated from the other SIG species by the absence of sucrose fermentation, from S. intermedius DSM 20373
T , S. pseudintermedius CCUG 49543T and S. cornubiensis DSM 105366T by the absence of methyl β-d-glucopyranoside fermentation and from S. delphini DSM 20771T by fermentation of trehalose. DNA relatedness of the type strain MI 10-1553T with the type strains of S. delphini , S. pseudintermedius , S. intermedius and S. cornubiensis was ≤48.2 % by digital DNA-DNA hybridization and ≤92.3 % by average nucleotide identity calculations. Iso-C15:0 , anteiso-C15 : 0 and anteiso-C17 : 0 were the most common fatty acids. Polar lipids consisted of phosphadidylglycerols, phospholipids, glycolipid, diphosphatidylglycerol and aminophospholipid. Cell-wall peptidoglycan was of type A3α l-Lys-Gly3 (Ser; similar to A11.2 and A11.3). The respiratory quinone belonged to menaquinone 7 (MK-7). The G+C content of MI 10-1553T was 39.3 mol%. The isolated strains represent a novel species of the genus Staphylococcus , for which we propose the name Staphylococcus ursi sp. nov. The type strain is MI 10-1553T (=ATCC TSD-55T =CCOS 1900T ).- Published
- 2020
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