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Staphylococcus pseudintermedius 5'-nucleotidase suppresses canine phagocytic activity.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2020 Jul; Vol. 246, pp. 108720. Date of Electronic Publication: 2020 May 16. - Publication Year :
- 2020
-
Abstract
- Staphylococcus pseudintermedius is a major opportunistic bacterial pathogen and the leading cause of pyoderma in dogs. In canines it is also often associated with infections of the urinary system and wounds and occasionally infects people. Widespread antimicrobial resistance has made the development of alternative treatments a high priority. The development of a staphylococcal vaccine, however, has proven challenging. Identification of virulence factors that inhibit phagocytosis and avoid innate immunity may play a significant role in preventing or treating infection with S. pseudintermedius. In this study, we identified a putative 5'-nucleotidase provisionally named SpAdsA, a S. pseudintermedius cell- wall protein encoded by SpAdsA. SpAdsA shares approximately 52% identity with the orthologous protein of Staphylococcus aureus and 14.8% identity with that of Streptococcus suis type2. It catalyzes the dephosphorylation of adenosine triphosphate and attenuation of this enzyme with critical amino acid substitutions nearly eliminated its hydrolytic activity. Exogenous adenosine inhibited phagocytosis of S. pseudintermedius by canine neutrophils and monocytes. Conversely, the addition of SpAdsA inhibitor or A <subscript>2A</subscript> adenosine receptor antagonist impaired the capacity of S. pseudintermedius to escape from killing by phagocytic cells. The neutralizing ability of canine antibody produced against SpAdsA-M was determined. Taken together, these results suggest that SpAdsA likely plays an important role in S. pseudintermedius virulence and that attenuated SpAdsA may be a good candidate for inclusion in a vaccine against S. pseudintermedius.<br />Competing Interests: Declaration of Competing Interest The authors (MA, DB, SK) have a patent pending for a vaccine composition that includes S. pseudintermedius 5′-nucleotidase.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- 5'-Nucleotidase genetics
Adenosine pharmacology
Adenosine Triphosphate metabolism
Animals
Bacterial Proteins genetics
Bacterial Proteins immunology
Cell Wall chemistry
Dogs
Phagocytes immunology
Phosphorylation
Staphylococcus genetics
Virulence Factors
5'-Nucleotidase immunology
Phagocytes microbiology
Phagocytosis
Staphylococcus enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 246
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32605759
- Full Text :
- https://doi.org/10.1016/j.vetmic.2020.108720