1. Design and evaluation of Raman reporters for the Raman-silent region
- Author
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Konstantinos Plakas, Lauren E. Rosch, Michael D. Clark, Shukree Adbul-Rashed, Travis M. Shaffer, Stefan Harmsen, Sanjiv S. Gambhir, and Michael R. Detty
- Subjects
inorganic chemicals ,surface-enhanced Raman scattering ,Biomedical Engineering ,Medicine (miscellaneous) ,Spectrum Analysis, Raman ,Alkynes ,triple bonds ,Raman-silent region ,Nanoparticles ,Gold ,Nanotag ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Research Paper ,Biotechnology - Abstract
Rationale: Surface enhanced Raman scattering (SERS) is proving to be a useful tool for biomedical imaging. However, this imaging technique can suffer from poor signal-to-noise ratio, as the complexity of biological tissues can lead to overlapping of Raman bands from tissues and the Raman reporter molecule utilized. Methods: Herein we describe the synthesis of triple bond containing Raman reporters that scatter light in the biological silent window, between 1750 cm-1 and 2750 cm-1. Results: Our SERS nanoprobes are comprised of uniquely designed Raman reporters containing either alkyne- or cyano-functional groups, enabling them to be readily distinguished from background biological tissue. Conclusion: We identify promising candidates that eventually can be moved forward as Raman reporters in SERS nanoparticles for highly specific contrast-enhanced Raman-based disease or analyte detection in biological applications.
- Published
- 2022