9 results on '"Jones-Engel, Lisa"'
Search Results
2. A novel Bayesian method for detection of APOBEC3-mediated hypermutation and its application to zoonotic transmission of simian foamy viruses.
- Author
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Matsen FA 4th, Small CT, Soliven K, Engel GA, Feeroz MM, Wang X, Craig KL, Hasan MK, Emerman M, Linial ML, and Jones-Engel L
- Subjects
- APOBEC Deaminases, APOBEC-3G Deaminase, Animals, Bangladesh, Bayes Theorem, Codon, Terminator, Computational Biology, Cytidine Deaminase genetics, Host-Pathogen Interactions genetics, Humans, Macaca genetics, Macaca virology, Models, Genetic, RNA, Viral genetics, Simian foamy virus pathogenicity, Simian foamy virus physiology, Species Specificity, Virus Replication, Cytosine Deaminase genetics, Mutation, Retroviridae Infections genetics, Retroviridae Infections transmission, Simian foamy virus genetics, Zoonoses genetics, Zoonoses transmission
- Abstract
Simian Foamy Virus (SFV) can be transmitted from non-human primates (NHP) to humans. However, there are no documented cases of human to human transmission, and significant differences exist between infection in NHP and human hosts. The mechanism for these between-host differences is not completely understood. In this paper we develop a new Bayesian approach to the detection of APOBEC3-mediated hypermutation, and use it to compare SFV sequences from human and NHP hosts living in close proximity in Bangladesh. We find that human APOBEC3G can induce genetic changes that may prevent SFV replication in infected humans in vivo.
- Published
- 2014
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3. New World simian foamy virus infections in vivo and in vitro.
- Author
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Stenbak CR, Craig KL, Ivanov SB, Wang X, Soliven KC, Jackson DL, Gutierrez GA, Engel G, Jones-Engel L, and Linial ML
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- Animals, Base Sequence, Cell Line, Humans, Macaca mulatta, Molecular Sequence Data, Platyrrhini, Simian foamy virus genetics, Simian foamy virus isolation & purification, Zoonoses transmission, Zoonoses virology, Monkey Diseases virology, Retroviridae Infections veterinary, Retroviridae Infections virology, Simian foamy virus physiology
- Abstract
Foamy viruses (FV) are complex retroviruses that naturally infect all nonhuman primates (NHP) studied to date. Zoonotic transmission of Old World NHP simian foamy viruses (SFV) has been documented, leading to nonpathogenic persistent infections. To date, there have been no reports concerning zoonotic transmission of New World monkey (NWM) SFV to humans and resulting infection. In this study, we developed a Western blot assay to detect antibodies to NWM SFV, a nested PCR assay to detect NWM SFV DNA, and a β-galactosidase-containing indicator cell line to assay replication of NWM SFV. Using these tools, we analyzed the plasma and blood of 116 primatologists, of whom 69 had reported exposures to NWM. While 8 of the primatologists tested were seropositive for SFV from a NWM, the spider monkey, none had detectable levels of viral DNA in their blood. We found that SFV isolated from three different species of NWM replicated in some, but not all, human cell lines. From our data, we conclude that while humans exposed to NWM SFV produce antibodies, there is no evidence for long-term viral persistence.
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- 2014
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4. Simian foamy virus infection of rhesus macaques in Bangladesh: relationship of latent proviruses and transcriptionally active viruses.
- Author
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Soliven K, Wang X, Small CT, Feeroz MM, Lee EG, Craig KL, Hasan K, Engel GA, Jones-Engel L, Matsen FA 4th, and Linial ML
- Subjects
- Animals, Bangladesh, Cheek virology, Female, Gene Products, gag genetics, Leukocytes, Mononuclear virology, Male, Proviruses isolation & purification, Proviruses physiology, RNA, Viral genetics, Retroviridae Infections virology, Simian foamy virus isolation & purification, Simian foamy virus physiology, Virus Replication, Macaca mulatta virology, Primate Diseases virology, Proviruses genetics, Retroviridae Infections veterinary, Simian foamy virus genetics, Transcription, Genetic, Virus Latency
- Abstract
Simian foamy viruses (SFV) are complex retroviruses that are ubiquitous in nonhuman primates (NHP) and are zoonotically transmitted to humans, presumably through NHP saliva, by licking, biting, and other behaviors. We have studied SFV in free-ranging rhesus macaques in Bangladesh. It has been previously shown that SFV in immunocompetent animals replicates to detectable levels only in superficial epithelial cells of the oral mucosa, although latent proviruses are found in most, if not all, tissues. In this study, we compare DNA sequences from latent SFV proviruses found in blood cells of 30 Bangladesh rhesus macaques to RNA sequences of transcriptionally active SFV from buccal swabs obtained from the same animals. Viral strains, defined by differences in SFV gag sequences, from buccal mucosal specimens overlapped with those from blood samples in 90% of animals. Thus, latent proviruses in peripheral blood mononuclear cells (PBMC) are, to a great extent, representative of viruses likely to be transmitted to other hosts. The level of SFV RNA in buccal swabs varied greatly between macaques, with increasing amounts of viral RNA in older animals. Evidence of APOBEC3-induced mutations was found in gag sequences derived from the blood and oral mucosa.
- Published
- 2013
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5. Diverse contexts of zoonotic transmission of simian foamy viruses in Asia.
- Author
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Jones-Engel L, May CC, Engel GA, Steinkraus KA, Schillaci MA, Fuentes A, Rompis A, Chalise MK, Aggimarangsee N, Feeroz MM, Grant R, Allan JS, Putra A, Wandia IN, Watanabe R, Kuller L, Thongsawat S, Chaiwarith R, Kyes RC, and Linial ML
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- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Ape Diseases transmission, Ape Diseases virology, Asia epidemiology, DNA, Viral genetics, Female, Humans, Male, Middle Aged, Occupational Exposure, Phylogeny, Retroviridae Infections epidemiology, Retroviridae Infections transmission, Simian foamy virus, Zoonoses transmission
- Abstract
In Asia, contact between persons and nonhuman primates is widespread in multiple occupational and nonoccupational contexts. Simian foamy viruses (SFVs) are retroviruses that are prevalent in all species of nonhuman primates. To determine SFV prevalence in humans, we tested 305 persons who lived or worked around nonhuman primates in several South and Southeast Asian countries; 8 (2.6%) were confirmed SFV positive by Western blot and, for some, by PCR. The interspecies interactions that likely resulted in virus transmission were diverse; 5 macaque taxa were implicated as a potential source of infection. Phylogenetic analysis showed that SFV from 3 infected persons was similar to that from the nonhuman primate populations with which the infected persons reported contact. Thus, SFV infections are likely to be prevalent among persons who live or work near nonhuman primates in Asia.
- Published
- 2008
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6. Sensitive assays for simian foamy viruses reveal a high prevalence of infection in commensal, free-ranging Asian monkeys.
- Author
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Jones-Engel L, Steinkraus KA, Murray SM, Engel GA, Grant R, Aggimarangsee N, Lee BP, May C, Schillaci MA, Somgird C, Sutthipat T, Vojtech L, Zhao J, and Linial ML
- Subjects
- Animals, Antibodies, Viral blood, Antibodies, Viral immunology, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Macaca, Neutralization Tests, Polymerase Chain Reaction, Prevalence, Sensitivity and Specificity, Simian foamy virus immunology, Simian foamy virus pathogenicity, Species Specificity, Virulence, Retroviridae Infections epidemiology, Simian foamy virus isolation & purification
- Abstract
Foamy viruses (FV) are retroviruses that naturally infect many hosts, including most nonhuman primates (NHPs). Zoonotic infection by primate FV has been documented in people in Asia who reported contact with free-ranging macaques. FV transmission in Asia is a concern, given abundant human-NHP contact, particularly at monkey temples and in urban settings. We have developed three assays capable of detecting the presence of FV in Asian NHP species that are commensal with humans: enzyme-linked immunosorbent assay (ELISA), Western blot assays using recombinant viral Gag protein, and an indicator cell line that can detect macaque FV. The recombinant ELISA correlates very well with the presence of FV sequences detected by PCR. We have used these assays to demonstrate both that FV is highly prevalent among free-ranging NHPs and that seroconversion occurs at a young age in these animals. These assays should also prove useful for large-scale analysis of the prevalence of FV infections in human populations in Asia that are commensal with free-ranging NHPs.
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- 2007
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7. Population dynamics of rhesus macaques and associated foamy virus in Bangladesh.
- Author
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Feeroz, Mostafa M, Soliven, Khanh, Small, Christopher T, Engel, Gregory A, Andreina Pacheco, M, Yee, JoAnn L, Wang, Xiaoxing, Kamrul Hasan, M, Oh, Gunwha, Levine, Kathryn L, Rabiul Alam, SM, Craig, Karen L, Jackson, Dana L, Lee, Eun-Gyung, Barry, Peter A, Lerche, Nicholas W, Escalante, Ananias A, Matsen Iv, Frederick A, Linial, Maxine L, and Jones-Engel, Lisa
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Bangladesh ,anthropogenic change ,emerging infectious diseases ,macaques ,simian foamy virus ,Microbiology - Abstract
Foamy viruses are complex retroviruses that have been shown to be transmitted from nonhuman primates to humans. In Bangladesh, infection with simian foamy virus (SFV) is ubiquitous among rhesus macaques, which come into contact with humans in diverse locations and contexts throughout the country. We analyzed microsatellite DNA from 126 macaques at six sites in Bangladesh in order to characterize geographic patterns of macaque population structure. We also included in this study 38 macaques owned by nomadic people who train them to perform for audiences. PCR was used to analyze a portion of the proviral gag gene from all SFV-positive macaques, and multiple clones were sequenced. Phylogenetic analysis was used to infer long-term patterns of viral transmission. Analyses of SFV gag gene sequences indicated that macaque populations from different areas harbor genetically distinct strains of SFV, suggesting that geographic features such as forest cover play a role in determining the dispersal of macaques and SFV. We also found evidence suggesting that humans traveling the region with performing macaques likely play a role in the translocation of macaques and SFV. Our studies found that individual animals can harbor more than one strain of SFV and that presence of more than one SFV strain is more common among older animals. Some macaques are infected with SFV that appears to be recombinant. These findings paint a more detailed picture of how geographic and sociocultural factors influence the spectrum of simian-borne retroviruses.
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- 2013
8. Risk assessment: a model for predicting cross-species transmission of simian foamy virus from macaques (M. fascicularis) to humans at a monkey temple in Bali, Indonesia.
- Author
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Engel, Gregory, Hungerford, Laura L., Jones-Engel, Lisa, Travis, Dominic, Eberle, Richard, Fuentes, Agustin, Grant, Richard, Kyes, Randall, and Schillaci, Michael
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RISK assessment ,INFECTIOUS disease transmission ,SIMIAN viruses ,MACAQUES ,PATHOGENIC microorganisms ,HEALTH risk assessment ,DISEASE research - Abstract
Contact between humans and nonhuman primates (NHPs) frequently occurs at monkey temples (religious sites that have become associated with free-ranging populations of NHPs) in Asia, creating the potential for NHP–human disease transmission. In March 2003 a multidisciplinary panel of experts participated in a workshop designed to model the risk of NHP–human pathogen transmission. The panel developed a risk assessment model to describe the likelihood of cross-species transmission of simian foamy virus (SFV) from temple macaques (Macaca fascicularis) to visitors at monkey temples. SFV is an enzootic simian retrovirus that has been shown to be transmitted from NHPs to humans. In operationalizing the model field data, laboratory data and expert opinions were used to estimate the likelihood of SFV transmission within this context. This model sets the stage for a discussion about modeling as a risk assessment tool and the kinds of data that are required to accurately predict transmission. Am. J. Primatol. 68:934–948, 2006. © 2006 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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9. Prevalence of enzootic simian viruses among urban performance monkeys in Indonesia.
- Author
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Schillaci, Michael A., Jones-Engel, Lisa, Engel, Gregory A., Paramastri, Yasmina, Iskandar, Entang, Wilson, Brenda, Allan, Jonathan S., Kyes, Randall C., Watanabe, Robin, and Grant, Richard
- Subjects
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SIMIAN viruses , *COMMUNICABLE diseases , *MONKEYS , *INFECTIOUS disease transmission , *INFECTION - Abstract
Animal reservoirs are the most important sources of emerging infectious diseases that threaten human populations. Global travel and tourism bring ever-increasing numbers of humans into contact with animals, increasing the likelihood of cross species transmission of infectious agents. Non-human primates come into contact with humans in a variety of contexts and may harbor infectious agents with zoonotic potential. We investigated the prevalence of infection with enzootic simian viruses among 20 urban performance monkeys ( Macaca fascicularis) in Jakarta, Indonesia. This report documents for the first time evidence of infection with four simian viruses in urban performance monkeys. Simian foamy virus was detected by PCR in 52.9% of the macaques. Antibodies to simian retrovirus were detected in 10.5% of the macaques. Antibodies to Cercopithecine Herpesvirus 1, were detected in 5.3% of the macaques. Similarly, antibodies to simian T-cell lymphotropic virus were detected in 5.3% of the macaques. No evidence of infection with simian immunodeficiency virus was detected in these macaques. These results suggest that urban performance monkeys are a reservoir for enzootic simian viruses known to be capable of infecting humans. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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