37 results on '"Bhatia, Madhav"'
Search Results
2. Substance P Promotes Leukocyte Infiltration in the Liver and Lungs of Mice with Sepsis: A Key Role for Adhesion Molecules on Vascular Endothelial Cells.
- Author
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Zhu Z, Chambers S, and Bhatia M
- Subjects
- Animals, Mice, Male, Leukocytes metabolism, Mice, Inbred C57BL, Peroxidase metabolism, Cell Adhesion Molecules metabolism, Cell Adhesion Molecules genetics, Disease Models, Animal, Sepsis metabolism, Sepsis pathology, Substance P metabolism, Lung metabolism, Lung pathology, Liver metabolism, Liver pathology, Intercellular Adhesion Molecule-1 metabolism, Intercellular Adhesion Molecule-1 genetics, Endothelial Cells metabolism, Vascular Cell Adhesion Molecule-1 metabolism, Vascular Cell Adhesion Molecule-1 genetics, Receptors, Neurokinin-1 metabolism, Receptors, Neurokinin-1 genetics
- Abstract
Substance P (SP), encoded by the Tac1 gene, has been shown to promote leukocyte infiltration and organ impairment in mice with sepsis. Neurokinin-1 receptor (NK1R) is the major receptor that mediates the detrimental impact of SP on sepsis. This investigation studied whether SP affects the expression of adhesion molecules, including intercellular cell adhesion molecule-1 (ICAM1) and vascular cell adhesion molecule-1 (VCAM1) on vascular endothelial cells in the liver and lungs, contributing to leukocyte infiltration in these tissues of mice with sepsis. Sepsis was induced by caecal ligation and puncture (CLP) surgery in mice. The actions of SP were inhibited by deleting the Tac1 gene, blocking NK1R, or combining these two methods. The activity of myeloperoxidase and the concentrations of ICAM1 and VCAM1 in the liver and lungs, as well as the expression of ICAM1 and VCAM1 on vascular endothelial cells in these tissues, were measured. The activity of myeloperoxidase and the concentration of ICAM1 and VCAM1 in the liver and lungs, as well as the expression of ICAM1 and VCAM1 on vascular endothelial cells in these tissues, increased in mice with CLP surgery-induced sepsis. Suppressing the biosynthesis of SP and its interactions with NK1R attenuated CLP surgery-induced alterations in the liver and lungs of mice. Our findings indicate that SP upregulates the expression of ICAM1 and VCAM1 on vascular endothelial cells in the liver and lungs, thereby increasing leukocyte infiltration in these tissues of mice with CLP surgery-induced sepsis by activating NK1R.
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- 2024
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3. Kupffer Cell Inactivation Alters Endothelial Cell Adhesion Molecules in Cecal Ligation and Puncture-Induced Sepsis.
- Author
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Manandhar S, Gaddam RR, Chambers S, and Bhatia M
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- Animals, Mice, Intercellular Adhesion Molecule-1, Vascular Cell Adhesion Molecule-1, Endothelial Cells, Punctures, Kupffer Cells, Sepsis
- Abstract
The activation of Kupffer cells, resident macrophages in the liver, is closely associated with the inflammatory response during sepsis, which leads to multiple-organ failure. However, how Kupffer cell activation affects adhesion molecules (ICAM-1 and VCAM-1) in sepsis has not been determined. This study investigated Kupffer cell inactivation's (by gadolinium chloride; GdCl
3 ) effects on adhesion molecule expression in CLP-induced sepsis. The induction of sepsis resulted in increased expression of liver and lung ICAM-1 and VCAM-1. GdCl3 pretreatment significantly decreased liver ICAM-1 expression but had no effect on VCAM-1 expression. In contrast, GdCl3 pretreatment had no effect on sepsis-induced increased adhesion molecule expression in the lungs. Similarly, the immunoreactivity of ICAM-1 was decreased in liver sinusoidal endothelial cells but increased in pulmonary endothelial cells in septic mice pretreated with GdCl3 . Further, GdCl3 pretreatment had no effect on the immunoreactivity of VCAM-1 in endothelial cells of the liver and lungs. Hence, the findings of this study demonstrate the differential effects of Kupffer cell inactivation on liver and lung adhesion molecules and suggest the complexity of their involvement in the pathophysiology of sepsis.- Published
- 2024
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4. Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells.
- Author
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Manandhar S, Chambers S, Miller A, Ishii I, and Bhatia M
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- Animals, Mice, Cell Adhesion Molecules, Endothelial Cells, Gene Deletion, NF-kappa B, Cystathionine gamma-Lyase antagonists & inhibitors, Cystathionine gamma-Lyase genetics, Sepsis drug therapy, Sepsis genetics
- Abstract
Hydrogen sulfide (H
2 S), synthesized by cystathionine gamma-lyase (Cth), contributes to the inflammatory response observed in sepsis. This study examines the effect of Cth-derived H2 S in adhesion molecules on endothelial cells of vital organs in mice in a cecal ligation puncture (CLP)-induced model of sepsis, using two different and complementary approaches: Cth gene deletion and pharmacological inhibition. Our findings revealed a decreased level of H2 S-synthesizing activity (via Cth) in both Cth-/- mice and PAG-treated wild-type (WT) mice following CLP-induced sepsis. Both treatment groups had reduced MPO activity and expression of chemokines (MCP-1 and MIP-2α), adhesion molecules (ICAM-1 and VCAM-1), ERK1/2 phosphorylation, and NF-κB in the liver and lung compared with in CLP-WT mice. Additionally, we found that PAG treatment in Cth-/- mice had no additional effect on the expression of ERK1/2 phosphorylation, NF-κB, or the production of chemokines and adhesion molecules in the liver and lung compared to Cth-/- mice following CLP-induced sepsis. The WT group with sepsis had an increased immunoreactivity of adhesion molecules on endothelial cells in the liver and lung than the WT sham-operated control. The Cth-/- , PAG-treated WT, and Cth-/- groups of mice showed decreased immunoreactivity of adhesion molecules on endothelial cells in the liver and lung following sepsis. Inhibition of H2 S production via both approaches reduced adhesion molecule expression on endothelial cells and reduced liver and lung injury in mice with sepsis. In conclusion, this study demonstrates that H2 S has an important role in the pathogenesis of sepsis and validates PAG use as a suited tool for investigating the Cth/H2 S-signalling axis in sepsis.- Published
- 2023
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5. Gases in Sepsis: Novel Mediators and Therapeutic Targets.
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Zhu Z, Chambers S, Zeng Y, and Bhatia M
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- Carbon Monoxide metabolism, Carbon Monoxide therapeutic use, Gases, Humans, Nitric Oxide metabolism, Gasotransmitters metabolism, Gasotransmitters therapeutic use, Hydrogen Sulfide metabolism, Hydrogen Sulfide therapeutic use, Sepsis drug therapy
- Abstract
Sepsis, a potentially lethal condition resulting from failure to control the initial infection, is associated with a dysregulated host defense response to pathogens and their toxins. Sepsis remains a leading cause of morbidity, mortality and disability worldwide. The pathophysiology of sepsis is very complicated and is not yet fully understood. Worse still, the development of effective therapeutic agents is still an unmet need and a great challenge. Gases, including nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H
2 S), are small-molecule biological mediators that are endogenously produced, mainly by enzyme-catalyzed reactions. Accumulating evidence suggests that these gaseous mediators are widely involved in the pathophysiology of sepsis. Many sepsis-associated alterations, such as the elimination of invasive pathogens, the resolution of disorganized inflammation and the preservation of the function of multiple organs and systems, are shaped by them. Increasing attention has been paid to developing therapeutic approaches targeting these molecules for sepsis/septic shock, taking advantage of the multiple actions played by NO, CO and H2 S. Several preliminary studies have identified promising therapeutic strategies for gaseous-mediator-based treatments for sepsis. In this review article, we summarize the state-of-the-art knowledge on the pathophysiology of sepsis; the metabolism and physiological function of NO, CO and H2 S; the crosstalk among these gaseous mediators; and their crucial effects on the development and progression of sepsis. In addition, we also briefly discuss the prospect of developing therapeutic interventions targeting these gaseous mediators for sepsis.- Published
- 2022
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6. Cystathionine-Gamma-Lyase-Derived Hydrogen Sulfide-Regulated Substance P Modulates Liver Sieve Fenestrations in Caecal Ligation and Puncture-Induced Sepsis.
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Gaddam RR, Chambers S, Fraser R, Cogger VC, Le Couteur DG, Ishii I, and Bhatia M
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- Animals, Endothelial Cells metabolism, Endothelial Cells pathology, Liver pathology, Lung metabolism, Lung pathology, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Receptors, Neurokinin-1 metabolism, Substance P genetics, Cystathionine gamma-Lyase metabolism, Liver metabolism, Sepsis metabolism, Substance P metabolism, Sulfites metabolism
- Abstract
Cystathionine-γ-lyase (CSE) isa hydrogen sulfide (H
2 S)-synthesizing enzyme that promotesinflammation by upregulating H2 S in sepsis. Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells (liver sieve) that undergo alteration during sepsis and H2 S plays a role in this process. Substance P (SP) is encoded by the preprotachykinin A (PPTA) gene, and promotes inflammation in sepsis; however, its regulation by H2 S is poorly understood. Furthermore, the interaction between H2 S and SP in modulating LSEC fenestrations following sepsis remains unclear. This study aimed to investigate whether CSE/H2 S regulates SP and the neurokinin-1 receptor (NK-1R) andmodulates fenestrations in LSECs following caecalligation and puncture (CLP)-induced sepsis. Here we report thatthe absence of either CSE or H2 S protects against liver sieve defenestration and gaps formation in LSECsin sepsis by decreased SP-NK-1R signaling. Following sepsis, there is an increased expression of liver CSE and H2 S synthesis, and plasma H2 S levels, which were aligned with higher SP levels in the liver, lungs and plasma and NK-1R in the liver and lungs. The genetic deletion of CSE led to decreased sepsis-induced SP and NK-1R in the liver, lungs and plasma SP suggesting H2 S synthesized through CSE regulates the SP-NK-1R pathway in sepsis. Further, mice deficient in the SP-encoding gene (PPTA) preservedsepsis-induced LSEC defenestrationand gaps formation, as seen by maintenance of patent fenestrations and fewer gaps. In conclusion, CSE/H2 S regulates SP-NK-1R and modulates LSEC fenestrations in sepsis.- Published
- 2019
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7. Circulating levels of hydrogen sulfide and substance P in patients with sepsis.
- Author
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Gaddam RR, Chambers S, Murdoch D, Shaw G, and Bhatia M
- Subjects
- Aged, C-Reactive Protein analysis, Calcitonin blood, Female, Humans, Intensive Care Units, Interleukin-6 blood, Male, Middle Aged, Protein Precursors blood, Sepsis drug therapy, Hydrogen Sulfide blood, Sepsis blood, Substance P blood
- Abstract
Objective: To determine alterations of circulating levels of hydrogen sulfide and substance P in patients with sepsis compared to non-sepsis patients with similar disease severity and organ dysfunction., Methods: This study included 23 septic and 14 non-septic patients during 2015-16 study period at the Christchurch Hospital Intensive Care Unit, Christchurch, New Zealand. Blood samples were collected from the time of admission to 96 h, with collection at different time points (0 h, 12 h, 24 h, 48 h, 72 h and 96 h) and subjected to measurement of hydrogen sulfide, substance P, procalcitonin, C-reactive protein, interleukin-6 and lactate levels., Results: Patients with sepsis showed higher circulating hydrogen sulfide and substance P levels compared to patients without sepsis. Hydrogen sulfide levels were significantly higher at 12 h (1.45 vs 0.75 μM; p < 0.05) and 24 h (1.11 vs 0.72 μM; p < 0.01), whereas substance P levels were higher at 48 h (0.55 vs 0.31 ng/mL; p < 0.05). Increased hydrogen sulfide and substance P levels in septic patients were associated with increased levels of inflammatory mediators - procalcitonin, C-reactive protein and interleukin-6., Conclusions: These results provide evidence that higher circulating levels of hydrogen sulfide and substance P are associated with increased inflammatory response in patients with sepsis., (Copyright © 2017 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2017
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8. Differential Effects of Kupffer Cell Inactivation on Inflammation and The Liver Sieve Following Caecal-Ligation and Puncture-Induced Sepsis in Mice.
- Author
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Gaddam RR, Fraser R, Badiei A, Chambers S, Cogger VC, Le Couteur DG, and Bhatia M
- Subjects
- Alanine Transaminase metabolism, Animals, Aspartate Aminotransferases metabolism, Chemokine CCL2 metabolism, Chemokine CXCL2 metabolism, Enzyme-Linked Immunosorbent Assay, Gadolinium toxicity, Inflammation immunology, Interleukin-1beta metabolism, Interleukin-6 metabolism, Kupffer Cells immunology, Liver injuries, Lung immunology, Lung metabolism, Lung Injury immunology, Lung Injury metabolism, Male, Mice, Mice, Inbred C57BL, Microscopy, Electron, Scanning, Peroxidase metabolism, Sepsis etiology, Tumor Necrosis Factor-alpha metabolism, Inflammation metabolism, Kupffer Cells metabolism, Liver immunology, Liver metabolism, Sepsis immunology, Sepsis metabolism
- Abstract
Sepsis remains a common clinical problem with significant mortality. Activation of the Kupffer cells during sepsis is associated with systemic inflammatory response and multiple organ failure. Kupffer cell activation also leads to structural changes in the liver sinusoidal endothelial cells (LSECs) during endotoxemia. However, these effects remain to be elucidated in caecal-ligation and puncture (CLP)-induced polymicrobial sepsis. To investigate the role of Kupffer cells on LSECs fenestrae and inflammation during CLP-induced sepsis, sepsis was induced by CLP and mice were treated with gadolinium chloride (GdCl3) before CLP-induced sepsis, to inactivate Kupffer cells. Mice were sacrificed after 8 h. Blood, liver, and lung tissues were collected and processed to measure LSECs fenestration, myeloperoxidase (MPO) activity, alanine transaminase (ALT) and aspartate aminotransferase (AST) activity, histological examination, and various cytokines/chemokines levels. LSECs fenestrae was studied using scanning electron micrographs of the LSECs. Strikingly, CLP mice treated with GdCl3 were protected against liver injury as evidenced by decreased LSECs defenestration and damage, MPO, ALT and AST activities, liver tissue damage, and inflammatory cytokines TNF-α, IL-6 and IL-1β, and chemokines MCP-1 and MIP-2α. However, CLP mice treated with GdCl3 had no protection against increased lung MPO activity, tissue damage, inflammatory cytokines, and chemokines. Treatment with GdCl3 also had no effect on the systemic inflammatory response as shown by no change in the circulatory inflammatory cytokines and chemokines following CLP-induced sepsis. Collectively, these data suggest that inactivation of Kupffer cells by GdCl3 protects the liver but had no effect on lung injury or inflammation and systemic inflammatory response following CLP-induced sepsis.
- Published
- 2017
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9. Cystathionine-Gamma-Lyase Gene Deletion Protects Mice against Inflammation and Liver Sieve Injury following Polymicrobial Sepsis.
- Author
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Gaddam RR, Fraser R, Badiei A, Chambers S, Cogger VC, Le Couteur DG, Ishii I, and Bhatia M
- Subjects
- Animals, Chemokines metabolism, Cytokines metabolism, Disease Models, Animal, Liver Diseases etiology, Liver Diseases metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Pneumonia etiology, Pneumonia metabolism, Sepsis microbiology, Cystathionine gamma-Lyase physiology, Gene Deletion, Liver Diseases prevention & control, Pneumonia prevention & control, Protective Agents metabolism, Sepsis complications
- Abstract
Background: Hydrogen sulfide (H2S), produced by the activity of cystathionine-gamma-lyase (CSE), is a key mediator of inflammation in sepsis. The liver sinusoidal endothelial cells (LSECs) are important target and mediator of sepsis. The aim of this study was to investigate the role of CSE-derived H2S on inflammation and LSECs fenestrae in caecal-ligation and puncture (CLP)-induced sepsis using CSE KO mice., Methods: Sepsis was induced by CLP, and mice (C57BL/6J, male) were sacrificed after 8 hours. Liver, lung, and blood were collected and processed to measure CSE expression, H2S synthesis, MPO activity, NF-κB p65, ERK1/2, and cytokines/chemokines levels. Diameter, frequency, porosity and gap area of the liver sieve were calculated from scanning electron micrographs of the LSECs., Results: An increased CSE expression and H2S synthesizing activity in the liver and lung of wild-type mice following CLP-induced sepsis. This was associated with an increased liver and lung MPO activity, and increased liver and lung and plasma levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β, and the chemokines MCP-1 and MIP-2α. Conversely, CSE KO mice had less liver and lung injury and reduced inflammation following CLP-induced sepsis as evidenced by decreased levels of H2S synthesizing activity, MPO activity, and pro-inflammatory cytokines/chemokines production. Extracellular-regulated kinase (ERK1/2) and nuclear factor-κB p65 (NF-κB) became significantly activated after the CLP in WT mice but not in CSE KO mice. In addition, CLP-induced damage to the LSECs, as indicated by increased defenestration and gaps formation in the LSECs compared to WT sham control. CSE KO mice showed decreased defenestration and gaps formation following sepsis., Conclusions: Mice with CSE (an H2S synthesising enzyme) gene deletion are less susceptible to CLP-induced sepsis and associated inflammatory response through ERK1/2-NF-κB p65 pathway as evidenced by reduced inflammation, tissue damage, and LSECs defenestration and gaps formation.
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- 2016
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10. Hydrogen sulfide upregulates cyclooxygenase-2 and prostaglandin E metabolite in sepsis-evoked acute lung injury via transient receptor potential vanilloid type 1 channel activation.
- Author
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Ang SF, Sio SW, Moochhala SM, MacAry PA, and Bhatia M
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- Acute Lung Injury chemically induced, Acute Lung Injury enzymology, Animals, Cecum, Cyclooxygenase 2 metabolism, Cyclooxygenase 2 physiology, Dinoprostone antagonists & inhibitors, Dinoprostone metabolism, Ligation, Lung enzymology, Lung metabolism, Male, Mice, Punctures, Sepsis complications, Sepsis enzymology, TRPV Cation Channels metabolism, Up-Regulation drug effects, Acute Lung Injury metabolism, Cyclooxygenase 2 biosynthesis, Dinoprostone biosynthesis, Hydrogen Sulfide pharmacology, Lung pathology, Sepsis metabolism, TRPV Cation Channels biosynthesis, Up-Regulation immunology
- Abstract
Hydrogen sulfide (H(2)S) has been shown to promote transient receptor potential vanilloid type 1 (TRPV1)-mediated neurogenic inflammation in sepsis and its associated multiple organ failure, including acute lung injury (ALI). Accumulating evidence suggests that the cyclooxygenase-2 (COX-2)/PGE(2) pathway plays an important role in augmenting inflammatory immune response in sepsis and respiratory diseases. However, the interactions among H(2)S, COX-2, and PGE(2) in inciting sepsis-evoked ALI remain unknown. Therefore, the aim of this study was to investigate whether H(2)S would upregulate COX-2 and work in conjunction with it to instigate ALI in a murine model of polymicrobial sepsis. Polymicrobial sepsis was induced by cecal ligation and puncture (CLP) in male Swiss mice. dl-propargylglycine, an inhibitor of H(2)S formation, was administrated 1 h before or 1 h after CLP, whereas sodium hydrosulfide, an H(2)S donor, was given during CLP. Mice were treated with TRPV1 antagonist capsazepine 30 min before CLP, followed by assessment of lung COX-2 and PGE(2) metabolite (PGEM) levels. Additionally, septic mice were administrated with parecoxib, a selective COX-2 inhibitor, 20 min post-CLP and subjected to ALI and survival analysis. H(2)S augmented COX-2 and PGEM production in sepsis-evoked ALI by a TRPV1 channel-dependent mechanism. COX-2 inhibition with parecoxib attenuated H(2)S-augmented lung PGEM production, neutrophil infiltration, edema, proinflammatory cytokines, chemokines, and adhesion molecules levels, restored lung histoarchitecture, and protected against CLP-induced lethality. The strong anti-inflammatory and antiseptic actions of selective COX-2 inhibitor may provide a potential therapeutic approach for the management of sepsis and sepsis-associated ALI.
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- 2011
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11. Hydrogen sulfide and neurogenic inflammation in polymicrobial sepsis: involvement of substance P and ERK-NF-κB signaling.
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Ang SF, Moochhala SM, MacAry PA, and Bhatia M
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- Animals, Capsaicin analogs & derivatives, Capsaicin pharmacology, Cell Nucleus metabolism, Chemokines metabolism, Cytokines metabolism, Inflammation, Male, Mice, Phosphorylation, Extracellular Signal-Regulated MAP Kinases metabolism, Hydrogen Sulfide pharmacology, NF-kappa B metabolism, Sepsis drug therapy, Sepsis microbiology, Substance P metabolism, TRPV Cation Channels metabolism
- Abstract
Hydrogen sulfide (H(2)S) has been shown to induce transient receptor potential vanilloid 1 (TRPV1)-mediated neurogenic inflammation in polymicrobial sepsis. However, endogenous neural factors that modulate this event and the molecular mechanism by which this occurs remain unclear. Therefore, this study tested the hypothesis that whether substance P (SP) is one important neural element that implicates in H(2)S-induced neurogenic inflammation in sepsis in a TRPV1-dependent manner, and if so, whether H(2)S regulates this response through activation of the extracellular signal-regulated kinase-nuclear factor-κB (ERK-NF-κB) pathway. Male Swiss mice were subjected to cecal ligation and puncture (CLP)-induced sepsis and treated with TRPV1 antagonist capsazepine 30 minutes before CLP. DL-propargylglycine (PAG), an inhibitor of H(2)S formation, was administrated 1 hour before or 1 hour after sepsis, whereas sodium hydrosulfide (NaHS), an H(2)S donor, was given at the same time as CLP. Capsazepine significantly attenuated H(2)S-induced SP production, inflammatory cytokines, chemokines, and adhesion molecules levels, and protected against lung and liver dysfunction in sepsis. In the absence of H(2)S, capsazepine caused no significant changes to the PAG-mediated attenuation of lung and plasma SP levels, sepsis-associated systemic inflammatory response and multiple organ dysfunction. In addition, capsazepine greatly inhibited phosphorylation of ERK(1/2) and inhibitory κBα, concurrent with suppression of NF-κB activation even in the presence of NaHS. Furthermore, capsazepine had no effect on PAG-mediated abrogation of these levels in sepsis. Taken together, the present findings show that H(2)S regulates TRPV1-mediated neurogenic inflammation in polymicrobial sepsis through enhancement of SP production and activation of the ERK-NF-κB pathway.
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- 2011
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12. The role of pro-inflammatory molecules and pharmacological agents in acute pancreatitis and sepsis.
- Author
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Shanmugam MK and Bhatia M
- Subjects
- Animals, Apoptosis, Ceruletide immunology, Chemokines immunology, Female, Humans, Hydrogen Sulfide toxicity, Integrins immunology, Male, Mice, Pancreatitis, Acute Necrotizing chemically induced, Pancreatitis, Acute Necrotizing pathology, Rats, Selectins immunology, Sepsis chemically induced, Sepsis pathology, Substance P immunology, Inflammation Mediators immunology, Pancreatitis, Acute Necrotizing immunology, Sepsis immunology
- Abstract
Acute pancreatitis (AP) and sepsis are inflammatory disorder varying in magnitude of response to infection or inflammatory stimuli. The specific role of various causative factors in AP, septic shock, current pharmacological treatments, animal models, role of infiltrating cells and novel molecules that play an important role in the disease progression to sepsis are explored. AP is an inflammatory disease of the pancreas. Over the years accumulating evidence suggests numerous molecules as key regulators of the inflammatory signaling cascade such as selectins, chemokine signaling and expression of intergrins on leukocytes facilitate adhesion to vessel walls. Inhibition of any of these molecules has proven to be effective in animal models of AP. Recently, the biochemical role of hydrogen sulfide (H(2)S) and substance P in caerulein induced AP and in cecal ligation and puncture induced sepsis and their role in the pathogenesis of the disease have highlighted the importance of novel molecules as therapeutic targets in addition to the known pro-inflammatory molecules, cytokines and chemoattractant chemokines and their receptors upregulated in AP and sepsis. This review aims to give an overview of the multifaceted complex interactions in a prearranged fashion and their functional role in the inflammatory process that afflict AP and sepsis. The interlinking molecules in AP and sepsis emphasize the similarities in the inflammatory response and the importance of pharmacological agents that reduce or inhibit the progression to chronic stage.
- Published
- 2010
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13. Hydrogen sulfide promotes transient receptor potential vanilloid 1-mediated neurogenic inflammation in polymicrobial sepsis.
- Author
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Ang SF, Moochhala SM, and Bhatia M
- Subjects
- Alkynes pharmacology, Animals, Capsaicin analogs & derivatives, Capsaicin pharmacology, Cystathionine gamma-Lyase metabolism, Glycine analogs & derivatives, Glycine pharmacology, Hydrogen Sulfide antagonists & inhibitors, Hydrogen Sulfide blood, Liver metabolism, Liver physiopathology, Lung metabolism, Lung physiopathology, Male, Mice, Multiple Organ Failure metabolism, Peroxidase metabolism, Sepsis physiopathology, Sulfides pharmacology, TRPV Cation Channels physiology, Transient Receptor Potential Channels antagonists & inhibitors, Transient Receptor Potential Channels physiology, Hydrogen Sulfide metabolism, Neurogenic Inflammation physiopathology, Sepsis metabolism, TRPV Cation Channels drug effects, Transient Receptor Potential Channels drug effects
- Abstract
Objective: To investigate the interaction and involvement of hydrogen sulfide and transient receptor potential vanilloid type 1 in the pathogenesis of sepsis. Hydrogen sulfide has been demonstrated to be involved in many inflammatory states including sepsis. Its contribution in neurogenic inflammation has been suggested in normal airways and urinary bladder. However, whether endogenous hydrogen sulfide would induce transient receptor potential vanilloid type 1-mediated neurogenic inflammation in sepsis remains unknown., Design: Prospective, experimental study., Setting: Research laboratory., Subject: Male Swiss mice., Interventions: Mice were subjected to cecal ligation and puncture-induced sepsis and treated with transient receptor potential vanilloid type 1 antagonist capsazepine (15 mg/kg subcutaneous) 30 mins before cecal ligation and puncture. To investigate hydrogen sulfide-mediated neurogenic inflammation in sepsis, DL-propargylglycine (50 mg/kg intraperitoneal), an inhibitor of hydrogen sulfide formation was administrated 1 hr before or 1 hr after the induction of sepsis, whereas sodium hydrosulfide (10 mg/kg intraperitoneal), a hydrogen sulfide donor, was given at the same time as cecal ligation and puncture. Lung and liver myeloperoxidase activities, liver cystathionine-gamma-lyase activity, plasma hydrogen sulfide level, histopathological examination, and survival studies were determined after induction of sepsis., Measurements and Main Results: Capsazepine treatment attenuates significantly systemic inflammation and multiple organ damage caused by sepsis, and protects against sepsis-induced mortality. Similarly, administration of sodium hydrosulfide exacerbates but capsazepine reverses these deleterious effects. In the presence of DL-propargylglycine, capsazepine causes no significant changes to the attenuation of sepsis-associated systemic inflammation, multiple organ damage, and mortality. In addition, capsazepine has no effect on endogenous generation of hydrogen sulfide, suggesting that hydrogen sulfide is located upstream of transient receptor potential vanilloid type 1 activation, and may play a critical role in regulating the production and release of sensory neuropeptides in sepsis., Conclusions: The present study shows that hydrogen sulfide induces systemic inflammation and multiple organ damage characteristic of sepsis via transient receptor potential vanilloid type 1-mediated neurogenic inflammation.
- Published
- 2010
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14. Plasma cytokine profiles in preprotachykinin-A knockout mice subjected to polymicrobial sepsis.
- Author
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Hegde A, Uttamchandani M, Moochhala SM, and Bhatia M
- Subjects
- Analysis of Variance, Animals, Cecum surgery, Cytokines immunology, Disease Models, Animal, Male, Mice, Mice, Inbred BALB C, Mice, Knockout, Protein Precursors immunology, Sepsis genetics, Sepsis immunology, Tachykinins immunology, Cytokines blood, Protein Precursors genetics, Sepsis blood, Tachykinins genetics
- Abstract
During the course of polymicrobial sepsis, a range of pro- and antiinflammatory cytokines are produced by the host immune system. Successful recovery from sepsis involves striking a balance between these counteracting cytokines. We herein investigated the circulating cytokine profiles in preprotachykinin-A knockout (PPTA(-/-)) mice, which have been found to be protected significantly against microbial sepsis, by employing multiplexed bead-based suspension arrays for the measurement of 18 plasma cytokines. Four sets of PPTA(-/-) and wild-type mice, each with six mice, were subjected to cecal ligation and puncture-induced sepsis or a sham procedure and were killed at 1, 5, 8 and 24 h post surgery. The cytokine profiles revealed, rather interestingly, that both pro- and antiinflammatory cytokines were elevated in the knockout group in response to a septic challenge. The higher systemic levels of both pro- and antiinflammatory cytokines in PPTA(-/-) septic mice was similar to the increase that we observed earlier in lung tissue of PPTA(-/-) mice after induction of sepsis. Thus, elevated levels of both pro- and antiinflammatory mediators may act simultaneously and help to resolve the infectious assault at the early stages of sepsis without excessively damaging the host tissue in PPTA(-/-) mice. In addition, our results underline the importance of comprehensive clinical analysis of multiple biomarkers to provide a better prognostic tool.
- Published
- 2010
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15. Sepsis as a model of SIRS.
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Bhatia M, He M, Zhang H, and Moochhala S
- Subjects
- Animals, Disease Models, Animal, Inflammation Mediators physiology, Sepsis physiopathology, Systemic Inflammatory Response Syndrome physiopathology
- Abstract
Sepsis describes a complex clinical syndrome that results from the host inability to regulate the inflammatory response against infection. Despite more than 20 years of extensive study, sepsis and excessive systemic inflammatory response syndrome (SIRS) are still the leading cause of death in intensive care units. The clinical study of sepsis and new therapeutics remains challenging due to the complexity of this disease. Therefore, many animal models have been employed to investigate the pathogenesis of sepsis and to preliminarily test potential therapeutics. However, so far, most therapeutics that have shown promising results in animal models failed in human clinical trials. In this chapter we will present an overview of different experimental animal models of sepsis and compare their advantages and disadvantage. The studies in animal models have greatly improved our understanding about the inflammatory mediators in sepsis. In this chapter we will also highlight the roles of several critical mediators including TNF-a , IL-1b , IL-6, chemokines, substance P, hydrogen sulfide and activated protein C in animal models of sepsis as well as in clinical studies.
- Published
- 2009
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16. Administration of exogenous fractalkine, a CX3C chemokine, is capable of modulating inflammatory response in cecal ligation and puncture-induced sepsis.
- Author
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He M, Moochhala SM, Adhikari S, and Bhatia M
- Subjects
- Animals, CX3C Chemokine Receptor 1, Cell Adhesion drug effects, Chemokine CX3CL1 blood, Cytokines blood, Disease Models, Animal, Leukocyte Rolling drug effects, Lung metabolism, Lung pathology, Lung Injury pathology, Male, Mice, Peroxidase blood, RNA, Messenger biosynthesis, Sepsis pathology, Splanchnic Circulation drug effects, Time Factors, Chemokine CX3CL1 pharmacology, Down-Regulation drug effects, Lung Injury blood, Neutrophil Infiltration drug effects, Receptors, Chemokine biosynthesis, Sepsis blood
- Abstract
Fractalkine (FTK) is a unique member of the CX3C chemokine family by acting through the CX3CR1 receptor. Membrane-bound FTK acts like an adhesion molecule, whereas soluble FTK (sFTK) acts as a classic chemokine ligand. Whether this chemokine plays a role in sepsis is still not clear. Using a mouse model of cecal ligation and puncture (CLP)-induced sepsis, we found that FTK levels were elevated in plasma 24 h after CLP. Reverse transcription-polymerase chain reaction results showed that FTK messenger RNA levels were upregulated, whereas CX3CR1 messenger RNA levels were downregulated in lungs after CLP procedure. To study the role of FTK in lung injury during sepsis, we injected exogenous sFTK into the mice before the CLP procedure. We found that plasma FTK levels were further elevated by sFTK. Mice that were injected with FTK had a lower myeloperoxidase activity in lungs compared with the CLP group. Furthermore, macrophage inflammatory protein 2, IL-1beta, and IL-6 levels in lungs were reduced after the injection of FTK. Treatment with sFTK also attenuated lung morphological changes in histological sections. To find out whether sFTK had an effect on leukocyte rolling and adherence, intravital microscope was used. Results showed that sFTK significantly attenuated leukocyte adhesion but had little effect on leukocyte rolling in mesenteric microcirculation. Taken together, our findings suggest that FTK may be a novel chemokine that modulates neutrophil infiltration and chemokine and cytokine production during sepsis.
- Published
- 2009
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17. Endogenous hydrogen sulfide regulates inflammatory response by activating the ERK pathway in polymicrobial sepsis.
- Author
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Zhang H, Moochhala SM, and Bhatia M
- Subjects
- Animals, Bacteremia enzymology, Bacteremia metabolism, Bacteremia pathology, Cecum surgery, Enzyme Activation immunology, Hydrogen Sulfide blood, Inflammation Mediators blood, Ligation, Male, Mice, Mitogen-Activated Protein Kinase 1 physiology, Mitogen-Activated Protein Kinase 3 physiology, NF-kappa B metabolism, NF-kappa B physiology, Peritonitis enzymology, Peritonitis metabolism, Peritonitis pathology, Punctures, Sepsis immunology, Sepsis metabolism, Up-Regulation immunology, Hydrogen Sulfide metabolism, Inflammation Mediators physiology, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3 metabolism, Sepsis enzymology, Sepsis pathology, Signal Transduction immunology
- Abstract
Hydrogen sulfide (H(2)S) up-regulates inflammatory response in several inflammatory diseases. However, to date, little is known about the molecular mechanism by which H(2)S provokes the inflammatory response in sepsis. Thus, the aim of this study was to investigate the signaling pathway underlying the proinflammatory role of H(2)S in cecal ligation and puncture (CLP)-induced sepsis. Male Swiss mice were subjected to CLP and treated with dl-propargylglycine (PAG; 50 mg/kg i.p., an inhibitor of H(2)S formation), NaHS (10 mg/kg, i.p., an H(2)S donor), or saline. PAG was administered 1 h before CLP, whereas NaHS was given at the time of CLP. CLP-induced sepsis resulted in a time-dependent increase in the synthesis of endogenous H(2)S. Maximum phosphorylation of ERK1/2 and degradation of IkappaBalpha in lung and liver were observed 4 h after CLP. Inhibition of H(2)S formation by PAG significantly reduced the phosphorylation of ERK1/2 in lung and liver 4 h after CLP, coupled with decreased degradation of IkappaBalpha and activation of NF-kappaB. In contrast, injection of NaHS significantly enhanced the activation of ERK1/2 in lung and liver, therefore leading to a further rise in tissue NF-kappaB activity. As a result, pretreatment with PAG significantly reduced the production of cytokines and chemokines in sepsis, whereas exogenous H(2)S greatly increased it. In addition, pretreatment with PD98059, an inhibitor of ERK kinase (MEK-1), significantly prevented NaHS from aggravating systemic inflammation in sepsis. In conclusion, the present study shows for the first time that H(2)S may regulate systemic inflammatory response in sepsis via ERK pathway.
- Published
- 2008
- Full Text
- View/download PDF
18. Role of MCP-1 in endotoxemia and sepsis.
- Author
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Ramnath RD, Ng SW, Guglielmotti A, and Bhatia M
- Subjects
- Animals, Calmodulin pharmacology, Endotoxemia chemically induced, Endotoxemia drug therapy, Indazoles therapeutic use, Lipopolysaccharides, Liver cytology, Liver drug effects, Liver metabolism, Lung cytology, Lung drug effects, Lung metabolism, Mice, Peritonitis drug therapy, Peroxidase metabolism, Propionates therapeutic use, Sepsis chemically induced, Sepsis drug therapy, Chemokine CCL2 metabolism, Endotoxemia metabolism, Indazoles pharmacology, Peritonitis metabolism, Propionates pharmacology, Sepsis metabolism
- Abstract
Sepsis is a complex clinical syndrome resulting from a harmful host inflammatory response to infection. Similarly, lipopolysaccharide (LPS) induced endotoxemia is marked by the activation of inflammatory responses, which can lead to shock, multiple organ damage and even death. Inflammatory mediator, chemokines are known to play an important role in the pathogenesis of sepsis and endotoxemia. Monocyte chemoattractant protein (MCP)-1, a prototype of CC chemokines, is a potent chemoattractant and a regulatory mediator involved in a variety of inflammatory diseases. The objective of this study is to investigate the role of MCP-1, by using bindarit, a blocker of MCP-1 synthesis, in murine models of sepsis and endotoxemia. Treatment with bindarit both prophylactically and therapeutically significantly (P<0.05) reduced MCP-1 levels in the lungs and liver in both sepsis and endotoxemia. In addition, prophylactic and therapeutic treatment with bindarit significantly (P<0.05) protected mice against sepsis and endotoxemia, as evidenced by the attenuation in lung and liver myeloperoxidase (MPO) activity, an indicator of neutrophil recruitment. The protective effect of bindarit was further confirmed by histological examination of lung and liver sections. Treatment with bindarit reduced lung and liver injury as indicated by decreased thickening of alveolar and neutrophil infiltration in CLP-induced sepsis and LPS-induced endotoxemia. Considering these results, we propose that anti-MCP-1 strategies may be of potential therapeutic value in the treatment of sepsis and endotoxemia.
- Published
- 2008
- Full Text
- View/download PDF
19. Endogenous hydrogen sulfide regulates leukocyte trafficking in cecal ligation and puncture-induced sepsis.
- Author
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Zhang H, Zhi L, Moochhala SM, Moore PK, and Bhatia M
- Subjects
- Alkynes pharmacology, Animals, Cell Adhesion drug effects, Cell Adhesion immunology, Cell Adhesion Molecules biosynthesis, Cell Adhesion Molecules immunology, Chemokine CXCL2 biosynthesis, Chemokine CXCL2 immunology, Enzyme Inhibitors pharmacology, Glycine analogs & derivatives, Glycine pharmacology, Hydrogen Sulfide antagonists & inhibitors, Hydrogen Sulfide metabolism, Inflammation Mediators antagonists & inhibitors, Inflammation Mediators metabolism, Leukocyte Rolling drug effects, Liver immunology, Liver metabolism, Liver pathology, Lung immunology, Lung metabolism, Lung pathology, Male, Mesenteric Veins immunology, Mesenteric Veins metabolism, Mesenteric Veins pathology, Mice, NF-kappa B antagonists & inhibitors, NF-kappa B immunology, NF-kappa B metabolism, Neutrophil Infiltration drug effects, Neutrophils metabolism, Neutrophils pathology, Nitriles pharmacology, Pneumonia immunology, Pneumonia metabolism, Pneumonia pathology, Receptors, Interleukin-8B biosynthesis, Receptors, Interleukin-8B immunology, Sepsis metabolism, Sepsis pathology, Sulfides pharmacology, Sulfones pharmacology, Up-Regulation drug effects, Up-Regulation immunology, Hydrogen Sulfide immunology, Inflammation Mediators immunology, Leukocyte Rolling immunology, Neutrophil Infiltration immunology, Neutrophils immunology, Sepsis immunology
- Abstract
Hydrogen sulfide (H(2)S) is recognized increasingly as a proinflammatory mediator in various inflammatory conditions. Here, we have investigated the role of H(2)S in regulating expression of some endothelial adhesion molecules and recruitment of leukocytes to inflamed sites in sepsis. Male Swiss mice were subjected to cecal ligation and puncture (CLP)-induced sepsis and treated with saline (i.p.), DL-propargylglycine (PAG; 50 mg/kg, i.p.), an inhibitor of H(2)S formation or NaHS (10 mg/kg, i.p.), an H(2)S donor. PAG was administered 1 h before or after the induction of sepsis, and NaHS was given at the same time of CLP. Using intravital microcopy, we found that in sepsis, prophylactic and therapeutic administration of PAG reduced leukocyte rolling and adherence significantly in mesenteric venules coupled with decreased mRNA and protein levels of adhesion molecules (ICAM-1, P-selectin, and E-selectin) in lung and liver. In contrast, injection of NaHS up-regulated leukocyte rolling and attachment significantly, as well as tissue levels of adhesion molecules in sepsis. Conversely, normal mice were given NaHS (10 mg/kg, i.p.) to induce lung inflammation, with or without NF-kappaB inhibitor BAY 11-7082 pretreatment. NaHS treatment enhanced the level of adhesion molecules and neutrophil infiltration in lung. These alterations were reversed by pretreatment with BAY 11-7082. Moreover, expression of CXCR2 in neutrophils obtained from H(2)S-treated mice was up-regulated significantly, leading to an obvious elevation in MIP-2-directed migration of neutrophils. Therefore, H(2)S acts as an important endogenous regulator of leukocyte activation and trafficking during an inflammatory response.
- Published
- 2007
- Full Text
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20. Hydrogen sulfide up-regulates substance P in polymicrobial sepsis-associated lung injury.
- Author
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Zhang H, Hegde A, Ng SW, Adhikari S, Moochhala SM, and Bhatia M
- Subjects
- Animals, Cecum surgery, Gene Deletion, Hydrogen Sulfide antagonists & inhibitors, Hydrogen Sulfide metabolism, Inflammation Mediators antagonists & inhibitors, Inflammation Mediators metabolism, Inflammation Mediators pharmacology, Ligation, Lung drug effects, Lung metabolism, Male, Mice, Mice, Inbred BALB C, Mice, Knockout, Neurokinin-1 Receptor Antagonists, Protein Precursors deficiency, Protein Precursors genetics, Punctures, Quinuclidines administration & dosage, Quinuclidines therapeutic use, Sepsis drug therapy, Sepsis genetics, Sulfides administration & dosage, Tachykinins deficiency, Tachykinins genetics, Hydrogen Sulfide pharmacology, Lung microbiology, Lung pathology, Sepsis metabolism, Sepsis microbiology, Substance P biosynthesis, Up-Regulation drug effects
- Abstract
Hydrogen sulfide (H2S) has been shown to induce the activation of neurogenic inflammation especially in normal airways and urinary bladder. However, whether endogenous H2S would regulate sepsis-associated lung inflammation via substance P (SP) and its receptors remains unknown. Therefore, the aim of the study was to investigate the effect of H2S on the pulmonary level of SP in cecal ligation and puncture (CLP)-induced sepsis and its relevance to lung injury. Male Swiss mice or male preprotachykinin-A gene knockout (PPT-A-/-) mice and their wild-type (PPT-A+/+) mice were subjected to CLP-induced sepsis. DL-propargylglycine (50 mg/kg i.p.), an inhibitor of H2S formation was administered either 1 h before or 1 h after the induction of sepsis, while NaHS, an H2S donor, was given at the same time as CLP. L703606, an inhibitor of the neurokinin-1 receptor was given 30 min before CLP. DL-propargylglycine pretreatment or posttreatment significantly decreased the PPT-A gene expression and the production of SP in lung whereas administration of NaHS resulted in a further rise in the pulmonary level of SP in sepsis. PPT-A gene deletion and pretreatment with L703606 prevented H2S from aggravating lung inflammation. In addition, septic mice genetically deficient in PPT-A gene or pretreated with L703606 did not exhibit further increase in lung permeability after injection of NaHS. The present findings show for the first time that in sepsis, H2S up-regulates the generation of SP, which contributes to lung inflammation and lung injury mainly via activation of the neurokinin-1 receptor.
- Published
- 2007
- Full Text
- View/download PDF
21. Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis.
- Author
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Hegde A, Zhang H, Moochhala SM, and Bhatia M
- Subjects
- Animals, Cecum drug effects, Cecum injuries, Chemokines metabolism, Cytokines metabolism, Enzyme-Linked Immunosorbent Assay, Lung Diseases immunology, Lung Diseases metabolism, Lung Injury, Male, Mice, Neutrophils metabolism, Peroxidase pharmacology, Protein Precursors metabolism, Receptors, Neurokinin-1 genetics, Receptors, Neurokinin-1 metabolism, Sepsis immunology, Sepsis metabolism, Stereoisomerism, Tachykinins metabolism, Lung drug effects, Lung Diseases drug therapy, Neurokinin-1 Receptor Antagonists, Piperidines pharmacology, Quinuclidines pharmacology, Sepsis drug therapy
- Abstract
Earlier work from our laboratory has suggested a role for the neuropeptide substance P (SP) in inducing lung injury in sepsis. In that study, mice lacking the preprotachykinin-A gene, which encodes for SP, were protected against lung injury in sepsis. To further substantiate the role of SP in sepsis and to study its mechanism, we have evaluated the effect of SR140333, a SP receptor antagonist, on lung injury in sepsis, which was induced in male Swiss mice by cecal ligation and puncture (CLP). Sham-operated animals received the same surgical procedure, except CLP. Vehicle or SR140333 (1 mg/kg, s.c.) was administered to CLP mice 30 min before or 1 h after the CLP. Eight hours after surgery, lung tissue was collected and analyzed for myeloperoxidase (MPO) activity, chemokines, cytokines, and adhesion molecules. The CLP procedure alone caused a significant increase in the lung levels of MIP-2, MCP-1, IL-1beta, IL-6, ICAM-1, E- and P-selectin, and MPO activity when compared with sham-operated mice. SR140333 injected 30 min before or 1 h after CLP significantly attenuated the increased lung MPO activity and levels of MIP-2, MCP-1, IL-1beta, IL-6, ICAM-1, and E- and P-selectin compared with CLP-operated mice injected with the vehicle. Histological evaluation of the lung sections further supported the beneficial effect of SR140333 on lung inflammation. Therefore, SP receptor antagonism can be a potential therapeutic target in polymicrobial sepsis, and this effect is brought about via reduction in leukocyte recruitment.
- Published
- 2007
- Full Text
- View/download PDF
22. Hydrogen sulfide acts as an inflammatory mediator in cecal ligation and puncture-induced sepsis in mice by upregulating the production of cytokines and chemokines via NF-kappaB.
- Author
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Zhang H, Zhi L, Moochhala S, Moore PK, and Bhatia M
- Subjects
- Alkynes pharmacology, Animals, Cystathionine gamma-Lyase antagonists & inhibitors, Disease Models, Animal, Glycine analogs & derivatives, Glycine pharmacology, Inflammation physiopathology, Ligation, Liver metabolism, Lung metabolism, Lung Diseases physiopathology, Male, Mice, Punctures, RNA, Messenger metabolism, Sulfides pharmacology, Up-Regulation, Cecum physiology, Chemokines biosynthesis, Cytokines biosynthesis, Hydrogen Sulfide pharmacology, Inflammation Mediators pharmacology, NF-kappa B physiology, Sepsis physiopathology
- Abstract
Recent studies have implied that hydrogen sulfide (H2S) plays a crucial role in several inflammatory conditions. However, so far little is known about the mechanism by which H2S provokes the inflammatory response in sepsis. Thus the aim of this study was to investigate if H2S regulates sepsis-associated systemic inflammation and production of proinflammatory mediators via the activation of NF-kappaB. Male Swiss mice were subjected to cecal ligation and puncture (CLP)-induced sepsis and treated with dl-propargylglycine (PAG; 50 mg/kg ip), NaHS (10 mg/kg ip), or saline. PAG, an inhibitor of H2S formation, was administered either 1 h before or 1 h after CLP, whereas NaHS, an H2S donor, was given at the time of CLP. Some normal mice were given NaHS (10 mg/kg ip) to induce lung inflammation with or without pretreatment with the NF-kappaB inhibitor BAY 11-7082. Eight hours after CLP, both prophylactic and therapeutic administration of PAG significantly reduced the mRNA and protein levels of IL-1beta, IL-6, TNF-alpha, monocyte chemotactic protein-1, and macrophage inflammatory protein-2 in lung and liver coupled with decreased activation and translocation of NF-kappaB in lung and liver. Inhibition of H2S formation also significantly reduced lung permeability and plasma alanine aminotransferase activity. In contrast, injection of NaHS significantly aggravated sepsis-associated systemic inflammation and increased NF-kappaB activation. In addition, H2S-induced lung inflammation was blocked by BAY 11-7082. Therefore, H2S upregulates the production of proinflammatory mediators and exacerbates the systemic inflammation in sepsis through a mechanism involving NF-kappaB activation.
- Published
- 2007
- Full Text
- View/download PDF
23. Treatment with BX471, a CC chemokine receptor 1 antagonist, attenuates systemic inflammatory response during sepsis.
- Author
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He M, Horuk R, Moochhala SM, and Bhatia M
- Subjects
- Animals, Anti-Inflammatory Agents therapeutic use, Appendix surgery, Chemokine CCL4, Disease Models, Animal, Disease Progression, E-Selectin genetics, E-Selectin metabolism, Gene Expression drug effects, Intercellular Adhesion Molecule-1 genetics, Intercellular Adhesion Molecule-1 metabolism, Ligation, Liver metabolism, Liver pathology, Lung metabolism, Lung pathology, Macrophage Inflammatory Proteins biosynthesis, Male, Mice, P-Selectin genetics, P-Selectin metabolism, Peroxidase metabolism, Phenylurea Compounds therapeutic use, Piperidines therapeutic use, RNA, Messenger metabolism, Receptors, CCR1, Receptors, Chemokine metabolism, Sepsis genetics, Sepsis metabolism, Sepsis pathology, Sepsis therapy, Time Factors, Anti-Inflammatory Agents pharmacology, Liver drug effects, Lung drug effects, Neutrophil Infiltration drug effects, Phenylurea Compounds pharmacology, Piperidines pharmacology, Receptors, Chemokine antagonists & inhibitors, Sepsis prevention & control
- Abstract
Sepsis is a complex clinical syndrome resulting from a harmful host inflammatory response to infection. Chemokines and their receptors play a key role in the pathogenesis of sepsis. BX471 is a potent nonpeptide CC chemokine receptor-1 (CCR1) antagonist in both human and mouse. The aim of the present study was to evaluate the effect of prophylactic and therapeutic treatment with BX471 on cecal ligation and puncture-induced sepsis in the mouse and to investigate the underlying mechanisms. In sepsis induced by cecal ligation and puncture, treatment with BX471 significantly protected mice against lung and liver injury by attenuating MPO activity, an indicator of neutrophil recruitment in lungs and livers and attenuating lung and liver morphological changes in histological sections. Blocking CCR1 by BX471 also downregulated ICAM-1, P-selectin, and E-selectin expression at mRNA and protein levels in lungs and livers compared with placebo-treated groups. These findings suggest that blockage of CCR1 by specific antagonist may represent a promising strategy to prevent disease progression in sepsis.
- Published
- 2007
- Full Text
- View/download PDF
24. Role of hydrogen sulfide in cecal ligation and puncture-induced sepsis in the mouse.
- Author
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Zhang H, Zhi L, Moore PK, and Bhatia M
- Subjects
- Alkynes pharmacology, Animals, Disease Models, Animal, Enzyme Inhibitors pharmacology, Glycine analogs & derivatives, Glycine pharmacology, Hydrogen Sulfide blood, Liver drug effects, Liver microbiology, Lung microbiology, Mice, Punctures, Sepsis etiology, Cecum physiology, Hydrogen Sulfide pharmacology, Liver pathology, Lung pathology, Sepsis physiopathology
- Abstract
Endogenous hydrogen sulfide (H(2)S) is naturally synthesized in various types of mammalian cells from l-cysteine in a reaction catalyzed by two enzymes, cystathionine-gamma-lyase (CSE) and/or cystathionine-beta-synthase. The latest studies have implied that H(2)S functions as a vasodilator and neurotransmitter. However, so far there is little information about the role played by H(2)S in systemic inflammation such as sepsis. Thus the aim of this study was to investigate the potential role of endogenous H(2)S in cecal ligation and puncture (CLP)-induced sepsis. Male Swiss mice were subjected to CLP-induced sepsis and treated with saline (ip), dl-propargylglycine (PAG, 50 mg/kg ip), a CSE inhibitor, or sodium hydrosulfide (NaHS; 10 mg/kg ip). PAG was administered either 1 h before or 1 h after the induction of sepsis, whereas NaHS was given at the same time of CLP. CLP-induced sepsis significantly increased the plasma H(2)S level and the liver H(2)S synthesis 8 h after CLP compared with sham operation. Induction of sepsis also resulted in a significant upregulation of CSE mRNA in liver. On the other hand, prophylactic as well as therapeutic administration of PAG significantly reduced sepsis-associated systemic inflammation, as evidenced by myeloperoxidase activity and histological changes in lung and liver, and attenuated the mortality of CLP-induced sepsis. Injection of NaHS significantly aggravated sepsis-associated systemic inflammation. Therefore, the effect of inhibition of H(2)S formation and administration of NaHS suggests that H(2)S plays a proinflammatory role in regulating the severity of sepsis and associated organ injury.
- Published
- 2006
- Full Text
- View/download PDF
25. Preprotachykinin-A gene products are key mediators of lung injury in polymicrobial sepsis.
- Author
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Puneet P, Hegde A, Ng SW, Lau HY, Lu J, Moochhala SM, and Bhatia M
- Subjects
- Animals, Cell Proliferation, Cytokines biosynthesis, Gene Deletion, Male, Mice, Mice, Knockout, Neutrophils cytology, Neutrophils metabolism, Protein Precursors deficiency, Respiratory Distress Syndrome complications, Respiratory Distress Syndrome pathology, Sepsis complications, Sepsis pathology, Survival Rate, Tachykinins deficiency, Time Factors, Protein Precursors genetics, Protein Precursors metabolism, Respiratory Distress Syndrome metabolism, Respiratory Distress Syndrome microbiology, Sepsis metabolism, Sepsis microbiology, Tachykinins genetics, Tachykinins metabolism
- Abstract
Preprotachykinin-A (PPT-A) gene products substance P and neurokinin-A have been shown to play an important role in neurogenic inflammation. To investigate the role of PPT-A gene products in lung injury in sepsis, polymicrobial sepsis was induced by cecal ligation and puncture in PPT-A gene-deficient mice (PPT-A(-/-)) and the wild-type control mice (PPT-A(+/+)). PPT-A gene deletion significantly protected against mortality, delayed the onset of lethality, and improved the long-term survival following cecal ligation and puncture-induced sepsis. PPT-A(-/-) mice also had significantly attenuated inflammation and damage in the lungs. The data suggest that deletion of the PPT-A gene may have contributed to the disruption in recruitment of inflammatory cells resulting in protection against tissue damage, as in these mice the sepsis-associated increase in chemokine levels is significantly attenuated.
- Published
- 2006
- Full Text
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26. Suppressing the Substance P-NK1R Signalling Protects Mice against Sepsis-Associated Acute Inflammatory Injury and Ferroptosis in the Liver and Lungs.
- Author
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Zhu, Zhixing, Chambers, Stephen, and Bhatia, Madhav
- Subjects
LUNGS ,SUBSTANCE P ,LIVER injuries ,MICE ,DELETION mutation ,IRON - Abstract
Substance P (SP), encoded by the TAC1/Tac1 gene, acts as a significant mediator in dysregulated systemic inflammatory response and associated organ injury in sepsis by activating the neurokinin-1 receptor (NK1R). This study investigated the impact of SP-NK1R signaling on ferroptosis in the liver and lungs of mice with sepsis. Sepsis was induced by caecal ligation puncture (CLP) surgery in mice. The SP-NK1R signaling was suppressed by Tac1 gene deletion, NK1R blockade, and a combination of these two approaches. The physiological conditions of mice were recorded. The profile of the SP-NK1R cascade, inflammatory response, ferroptosis, and tissue histology were investigated in the liver and lungs. Several manifestations of sepsis occurred in Tac1
+/+ mice during the development of sepsis. Notably, hypothermia became significant four hours after the induction of sepsis. In the liver and lungs of mice subjected to CLP surgery, the concentrations of SP and NK1R were upregulated. Additionally, the concentrations of pro-inflammatory mediators, including cytokines (IL-1β, IL-6, and TNF-α) and chemokines (MCP-1 and MIP-2), were increased. Moreover, ferroptosis was elevated, as evidenced by increased concentrations of iron and MDA and reduced concentrations of GSH, Nrf2, and Gpx4. Suppressing the SP-NK1R cascade significantly mitigated CLP-surgery-induced alterations in mice. Importantly, these three approaches used to suppress SP-NK1R signaling showed similar effects on protecting mice against sepsis. In conclusion, increased SP-mediated acute inflammatory response and injury in the liver and lungs in mice with CLP-surgery-induced sepsis was associated with elevated ferroptosis. The detrimental effect of SP on sepsis was predominantly mediated by NK1R. Therefore, the suppression of increased SP-NK1R signaling and ferroptosis may be a promising adjuvant therapeutic candidate for sepsis and associated acute liver and lung injury. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
27. Hydrogen Sulfide and Substance P Levels in Patients with Escherichia coli and Klebsiella pneumoniae Bacteraemia.
- Author
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Manandhar, Sumeet, Scott-Thomas, Amy, Harrington, Michael, Sinha, Priyanka, Pilbrow, Anna, Richards, Arthur Mark, Cameron, Vicky, Bhatia, Madhav, and Chambers, Stephen T.
- Subjects
SUBSTANCE P ,KLEBSIELLA pneumoniae ,HYDROGEN sulfide ,ESCHERICHIA coli ,URINARY tract infections ,BACTEREMIA - Abstract
Hydrogen sulfide (H
2 S) and substance P (SP) are known from animal models and in vitro studies as proinflammatory mediators. In this study, peripheral blood concentrations of H2 S and SP were measured in patients with Escherichia coli or Klebsiella pneumoniae bacteraemia. Fifty patients were recruited from general wards at Christchurch Hospital, during 2020–2021. Samples from age- and sex-matched healthy subjects previously recruited as controls for studies of cardiovascular disease were used as controls. The concentrations of H2 S were higher than controls on day 0, day 1, and day 2, and SP was higher than controls on all 4 days. The concentrations of H2 S were highest on day 0, whereas SP concentrations were higher on day 2 than other days. Interleukin-6 and C-reactive protein were significantly higher on day 0 and day 1, respectively. The concentrations of H2 S and SP did not differ between 15 non-septic (SIRS 0-1) and the 35 septic subjects (SIRS ≥ 2). Substance P concentrations were higher in subjects with abdominal infection than urinary tract infections on day 0 (p = 0.0002) and day 1 (p = 0.0091). In conclusion, the peak H2 S concentrations precede the SP peak in patients with Gram-negative bacteraemia, but this response varies with the site of infection. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
28. Hydrogen Sulfide in Inflammation: A Novel Mediator and Therapeutic Target.
- Author
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Bhatia, Madhav and Gaddam, Ravinder Reddy
- Subjects
- *
LIPOXINS , *HYDROGEN sulfide , *INFLAMMATORY mediators - Abstract
Significance: Inflammation is a normal response to injury, but uncontrolled inflammation can lead to several diseases. In recent years, research has shown endogenously synthesized hydrogen sulfide (H2S) to be a novel mediator of inflammation. This review summarizes the current understanding and recent advances of H2S role with respect to inflammation in different diseases. Recent Advances: Promising early results from clinical studies suggest an important role of H2S in human inflammatory disease. Critical Issues: Defining the precise mechanism by which H2S contributes to inflammation is a complex challenge, and there is active ongoing research that is focused on addressing this question. Most of this work has been conducted on animal models of human disease and isolated/cultured cells, and its translation to the clinic is another challenge in the area of H2S research. Future Directions: Defining the mechanism by which H2S acts as an inflammatory mediator will help us better understand different inflammatory diseases and help develop novel therapeutic approaches for these diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
29. Hydrogen Sulfide and Neurogenic Inflammation in Polymicrobial Sepsis: Involvement of Substance P and ERK-NF-kB Signaling.
- Author
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Ang, Seah-Fang, Moochhala, Shabbir M., MacAry, Paul A., and Bhatia, Madhav
- Subjects
HYDROGEN sulfide ,DEVELOPMENTAL neurobiology ,INFLAMMATION ,SEPSIS ,NF-kappa B ,CELLULAR signal transduction ,TRP channels - Abstract
Hydrogen sulfide (H
2 S) has been shown to induce transient receptor potential vanilloid 1 (TRPV1)-mediated neurogenic inflammation in polymicrobial sepsis. However, endogenous neural factors that modulate this event and the molecular mechanism by which this occurs remain unclear. Therefore, this study tested the hypothesis that whether substance P (SP) is one important neural element that implicates in H2 S-induced neurogenic inflammation in sepsis in a TRPV1-dependent manner, and if so, whether H2 S regulates this response through activation of the extracellular signal-regulated kinasenuclear factor-kB (ERK-NF-kB) pathway. Male Swiss mice were subjected to cecal ligation and puncture (CLP)-induced sepsis and treated with TRPV1 antagonist capsazepine 30 minutes before CLP. DL-propargylglycine (PAG), an inhibitor of H2 S formation, was administrated 1 hour before or 1 hour after sepsis, whereas sodium hydrosulfide (NaHS), an H2 S donor, was given at the same time as CLP. Capsazepine significantly attenuated H2 S-induced SP production, inflammatory cytokines, chemokines, and adhesion molecules levels, and protected against lung and liver dysfunction in sepsis. In the absence of H2 S, capsazepine caused no significant changes to the PAG-mediated attenuation of lung and plasma SP levels, sepsisassociated systemic inflammatory response and multiple organ dysfunction. In addition, capsazepine greatly inhibited phosphorylation of ERK1/2 and inhibitory kBa, concurrent with suppression of NF-kB activation even in the presence of NaHS. Furthermore, capsazepine had no effect on PAG-mediated abrogation of these levels in sepsis. Taken together, the present findings show that H2 S regulates TRPV1-mediated neurogenic inflammation in polymicrobial sepsis through enhancement of SP production and activation of the ERK-NF-kB pathway. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
30. Treatment with BX471, a CC chemokine receptor 1 antagonist, attenuates systemic inflammatory response during sepsis.
- Author
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Min He, Horuk, Richard, Moochhala, Shabbir M., and Bhatia, Madhav
- Subjects
SEPSIS ,BLOOD diseases ,CHEMOKINES ,NEUTROPHILS ,MESSENGER RNA - Abstract
Sepsis is a complex clinical syndrome resulting from a harmful host inflammatory response to infection. Chemokines and their receptors play a key role in the pathogenesis of sepsis. BX471 is a potent nonpeptide CC chemokine receptor-1 (CCR1) antagonist in both human and mouse. The aim of the present study was to evaluate the effect of prophylactic and therapeutic treatment with BX471 on cecal ligation and puncture-induced sepsis in the mouse and to investigate the underlying mechanisms. In sepsis induced by cecal ligation and puncture, treatment with BX471 significantly protected mice against lung and liver injury by attenuating MPO activity, an indicator of neutrophil recruitment in lungs and livers and attenuating lung and liver morphological changes in histological sections. Blocking CCR1 by BX471 also downregulated ICAM-1, P-selectin, and E-selectin expression at mRNA and protein levels in lungs and livers compared with placebo-treated groups. These findings suggest that blockage of CCR1 by specific antagonist may represent a promising strategy to prevent disease progression in sepsis. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
31. Hydrogen sulfide acts as an inflammatory mediator in cecal ligation and puncture-induced sepsis in mice by upregulating the production of cytokines and chemokines via NF-κB.
- Author
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Huili Zhang, Liang Zhi, Moochhala, Shabbir, Moore, Philip K., and Bhatia, Madhav
- Subjects
HYDROGEN sulfide ,LABORATORY mice ,SEPSIS ,BLOOD diseases ,CHEMOKINES ,CYTOKINES - Abstract
Recent studies have implied that hydrogen sulfide (H
2 S) plays a crucial role in several inflammatory conditions. However, so far little is known about the mechanism by which H2 S provokes the inflammatory response in sepsis. Thus the aim of this study was to investigate if H2 S regulates sepsis-associated systemic inflammation and production of proinflammatory mediators via the activation of NF-κB. Male Swiss mice were subjected to cecal ligation and puncture (CLP)-induced sepsis and treated with DL-propargylglycine (PAG; 50 mg/kg ip), NaHS (10 mg/kg ip), or saline. PAG, an inhibitor of H2 S formation, was administered either 1 h before or I h after CLP, whereas NaHS, an H2 S donor, was given at the time of CLP. Some normal mice were given NaHS (10 mg/kg ip) to induce lung inflammation with or without pretreatment with the NF-κB inhibitor BAY 11-7082. Eight hours after CLP, both prophylactic and therapeutic administration of PAG significantly reduced the mRNA and protein levels of lL-1β lL-6, TNF-α, monocyte chemotactic protein-1, and macrophage inflammatory protein-2 in lung and liver coupled with decreased activation and translocation of NF-κB in lung and liver. Inhibition of H2 S formation also significantly reduced lung permeability and plasma alanine aminotransferase activity. In contrast, injection of NaHS significantly aggravated sepsis-associated systemic inflammation and increased NF-κB activation. In addition, H2 S-induced lung inflammation was blocked by BAY 11-7082. Therefore, H2 S upregulates the production of proinflammatory mediators and exacerbates the systemic inflammation in sepsis through a mechanism involving NF-κB activation. [ABSTRACT FROM AUTHOR]- Published
- 2007
- Full Text
- View/download PDF
32. Inflammatory mediators in sepsis: Cytokines, chemokines, adhesion molecules and gases.
- Author
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Devi Ramnath, Raina, Weing, Siaw, He, Min, Sun, Jia, Zhang, Huili, Singh Bawa, Manmish, and Bhatia, Madhav
- Subjects
INFECTION ,MULTIPLE organ failure ,CYTOKINES ,SEPSIS ,CHEMOKINES ,NEUTROPHILS ,MOLECULES - Abstract
Sepsis is a systemic inflammatory response syndrome in response to severe infection. An overwhelming systemic response brought about by the release of various inflammatory mediators can lead to shock, multiple organ damage and death. Cytokines play an important role in the pathogenesis of sepsis and are regulated by a complex network of pro- and anti-inflammatory mediators. Various chemokines sequester neutrophils into the target organ, further augmenting inflammation. Chemokine receptor antagonism represents a major therapeutic approach against sepsis. Adhesion molecules mediate the migration of leukocytes towards the site of inflammation and their activation. Gaseous mediators such as nitric oxide and hydrogen sulfide are also involved in the pathogenesis of inflammation. Our review summarizes the current understanding of the roles of various inflammatory mediators in sepsis. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
33. Role of hydrogen sulfide in cecal ligation and puncture-induced sepsis in the mouse.
- Author
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Huili Zhang, Liang Zhi, Moore, Philip K., and Bhatia, Madhav
- Subjects
SEPSIS ,INFLAMMATION ,ABDOMEN ,BILIARY tract ,MESSENGER RNA ,HYDROGEN sulfide - Abstract
Endogenous hydrogen sulfide (H
2 S) is naturally synthesized in various types of mammalian cells from L-Cysteine in a reaction catalyzed by two enzymes, cystathionine-γ-lyase (CSE) and/or cystathionine-β-synthase. The latest studies have implied that H2 S functions as a vasodilator and neurotransmitter. However, so far there is little information about the role played by H2 S in systemic inflammation such as sepsis. Thus the aim of this study was to investigate the potential role of endogenous H2 S in cecal ligation and puncture (CLP)-induced sepsis. Male Swiss mice were subjected to CLP-induced sepsis and treated with saline (ip), DL-propargylglycine (PAG, 50 mg/kg ip), a CSE inhibitor, or sodium hydrosulfide (NaHS; 10 mg/kg ip). PAG was administered either 1 h before or 1 h after the induction of sepsis, whereas NaHS was given at the same time of CLP. CLP-induced sepsis significantly increased the plasma H2 5 level and the liver H2 S synthesis 8 h after CLP compared with sham operation. Induction of sepsis also resulted in a significant upregulation of CSE mRNA in liver. On the other hand, prophylactic as well as therapeutic administration of PAG significantly reduced sepsis-associated systemic inflammation, as evidenced by myeloperoxidase activity and histological changes in lung and liver, and attenuated the mortality of CLP-induced sepsis. Injection of NaHS significantly aggravated sepsis-associated systemic inflammation. Therefore, the effect of inhibition of H2 S formation and administration of NaHS suggests that H2 S plays a proinflammatory role in regulating the severity of sepsis and associated organ injury. [ABSTRACT FROM AUTHOR]- Published
- 2006
- Full Text
- View/download PDF
34. Hydrogen sulfide promotes transient receptor potential vanilloid 1-mediated neurogenic inflammation in polymicrobial sepsis.
- Author
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Seah-Fang Ang, Moochhala, Shabbir M., and Bhatia, Madhav
- Subjects
- *
SEPSIS , *HYDROGEN sulfide , *INFLAMMATION , *LABORATORY rats , *TRP channels - Abstract
The article presents a study on the involvement and interaction of hydrogen sulfide and transient receptor potential vanilloid type 1 in the pathologic process of sepsis. It states that male Swiss mice were treated with transient receptor potential vanilloid type 1 antagonist capsazepine 30 minutes before cecal ligation and puncture-induced sepsis. The result shows that capsazepine treatment attenuates the systemic inflammation and multiple organ damaged induced by hydrogen sulfide.
- Published
- 2010
- Full Text
- View/download PDF
35. Hydrogen Sulfide Upregulates Cyclooxygenase-2 and Prostaglandin E Metabolite in Sepsis-Evoked Acute Lung Injury via Transient Receptor Potential Vanilloid Type 1 Channel Activation.
- Author
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Seah-Fang Ang, Sio, Selena W. S., Moochhala, Shabbir M., MacAry, Paul A., and Bhatia, Madhav
- Subjects
- *
HYDROGEN sulfide , *CYCLOOXYGENASE 2 , *PROSTAGLANDINS E , *SEPSIS , *LUNG injuries - Abstract
Hydrogen sulfide (H2S) has been shown to promote transient receptor potential vanilloid type 1 (TRPV1)-mediated neurogenic inflammation in sepsis and its associated multiple organ failure, including acute lung injury (ALI). Accumulating evidence suggests that the cyclooxygenase-2 (COX-2)/PGE2 pathway plays an important role in augmenting inflammatory immune response in sepsis and respiratory diseases. However, the interactions among H2S, COX-2, and PGE2 in inciting sepsis-evoked ALI remain unknown. Therefore, the aim of this study was to investigate whether H2S would upregulate COX-2 and work in conjunction with it to instigate ALI in a murine model of polymicrobial sepsis. Polymicrobial sepsis was induced by cecal ligation and puncture (CLP) in male Swiss mice. dl-propargylglycine, an inhibitor of H2S formation, was administrated 1 h before or 1 h after CLP, whereas sodium hydrosulfide, an H2S donor, was given during CLP. Mice were treated with TRPV1 antagonist capsazepine 30 min before CLP, followed by assessment of lung COX-2 and PGE2 metabolite (PGEM) levels. Additionally, septic mice were administrated with parecoxib, a selective COX-2 inhibitor, 20 min post-CLP and subjected to ALI and survival analysis. H2S augmented COX-2 and PGEM production in sepsis-evoked ALI by a TRPV1 channel-dependent mechanism. COX-2 inhibition with parecoxib attenuated H2S-augmented lung PGEM production, neutrophil infiltration, edema, proinflammatory cytokines, chemokines, and adhesion molecules levels, restored lung histoarchitecture, and protected against CLP-induced lethality. The strong anti-inflammatory and antiseptic actions of selective COX-2 inhibitor may provide a potential therapeutic approach for the management of sepsis and sepsis-associated ALI. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
36. Role of Hydrogen Sulfide in Severe Burn Injury-Induced Inflammation in Mice.
- Author
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Jing Zhang, Selena Wei Shan Sio, Moochhala, Shabbir, and Bhatia, Madhav
- Subjects
- *
HYDROGEN sulfide , *ANIMAL models of wound healing , *LABORATORY mice , *MESSENGER RNA , *PANCREATITIS , *ENDOTOXEMIA , *SEPSIS , *THERAPEUTICS - Abstract
Endogenous hydrogen sulfide (H2S) is naturally synthesized in many types of mammalian cells from L-cysteine in the reactions catalyzed by cystathionine-β-synthase and cystathionine-γ-lyase (CSE). H2S has been demonstrated to play a proinflammatory role in various animal models of hindpaw edema, acute pancreatitis, lipopolysaccharide-induced endotoxemia and cecal ligation, and puncture-induced sepsis. Full-thickness burns that exceed 25% of the total body surface area (TBSA) produce a profound systemic inflammatory reaction characterized by leukocyte activation and plasma leakage in the microvasculature of tissues and organs remote from the wound. The aim of this study was to investigate the effect of local burn injury on induced distant organ endogenous H2S release and expression of CSE. Male BALB/c mice were subjected to 30% TBSA full-thickness burn and treated with saline (administered intraperitoneally [i.p.]); DL-propargylglycine (PAG, 50 mg/kg i.p.), which is a CSE inhibitor; or sodium hydrosulfide (NaHS, 10 mg/kg i.p.), which is an H2S donor. PAG was administered either 1 h before or 1 h after the burn injury, whereas NaHS was given at the same time as the burn injury. Measurements of liver myeloperoxidase (MPO) activities, liver H2S-synthesizing activity, plasma H2S level and liver and lung CSE mRNA expression and histological examination of tissues were performed after burn injury. Burn injury significantly increased the plasma H2S level and liver H2S synthesis 8 h after burn compared with the sham group. Burn injury also resulted in a significant upregulation of CSE mRNA in liver and lung. Prophylactic as well as therapeutic administration of PAG significantly reduced burn-associated systemic inflammation, as evidenced by MPO activity and histological changes in liver and lung. Injection of NaHS significantly aggravated burn-associated systemic inflammation. Therefore, our findings show for the first time the role of H2S in contributing to inflammatory damage after burn injury. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
37. Substance P in Polymicrobial Sepsis: Molecular Fingerprint of Lung Injury in Preprotachykinin-A-/- Mice.
- Author
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Hegde, Akhil, Tamizhselvi, Ramasamy, Manikandan, Jayapal, Melendez, Alirio J., Moochhala, Shabbir M., and Bhatia, Madhav
- Subjects
- *
SEPSIS , *LUNG injuries , *NEUROPEPTIDES , *MORTALITY , *GENE expression , *DNA fingerprinting , *IMMUNOREGULATION , *INTERLEUKIN-1 , *GENETICS - Abstract
Deletion of mouse preprotachykinin-A (PPTA), which encodes mainly for neuropeptide substance P, has been shown to protect against lung injury and mortality in sepsis. This study explored microarray-based differential gene expression profiles in mouse lung tissue 8 h after inducing microbial sepsis and the effect of PPTA gene deletion. A range of genes differentially expressed (more than two-fold) in microarray analysis was assessed, comparing wild-type and PPTA-knockout septic mice with their respective sham controls, and the data were further validated. Genetic deletion of substance P resulted in a significantly different expression profile of genes involved in inflammation and immunomodulation after the induction of sepsis, compared with wild-type mice. Interestingly, apart from the various proinflammatory mediators, the antiinflammatory cytokine interleukin-1 receptor antagonist gene (IL1RN) was also elevated much more in PPTA-1- septic mice. In addition, semiquantitative RT-PCR analysis supported the microarray data. The microarray data imply that the elevated levels of inflammatory gene expression in the early stages of sepsis in PPTA-knockout mice are possibly aimed to resolve the infection without excessive immunosuppression. As scientists are divided over the effects of pro- and antiinflammatory mediators in sepsis, it seems prudent to define the status depending on a complete genome profile. This is the first report exploring pulmonary gene expression profiles using microarray analysis in PPTA-knockout mice subjected to cecal ligation and puncture-induced sepsis and providing additional biological insight into the protection received against lung injury and mortality. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
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