8 results on '"Maghsoudlou, P"'
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2. Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration
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Anna Urciuolo, Luca Urbani, Silvia Perin, Panagiotis Maghsoudlou, Federico Scottoni, Asllan Gjinovci, Henry Collins-Hooper, Stavros Loukogeorgakis, Athanasios Tyraskis, Silvia Torelli, Elena Germinario, Mario Enrique Alvarez Fallas, Carla Julia-Vilella, Simon Eaton, Bert Blaauw, Ketan Patel, and Paolo De Coppi
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Medicine ,Science - Abstract
Abstract Pathological conditions affecting skeletal muscle function may lead to irreversible volumetric muscle loss (VML). Therapeutic approaches involving acellular matrices represent an emerging and promising strategy to promote regeneration of skeletal muscle following injury. Here we investigated the ability of three different decellularised skeletal muscle scaffolds to support muscle regeneration in a xenogeneic immune-competent model of VML, in which the EDL muscle was surgically resected. All implanted acellular matrices, used to replace the resected muscles, were able to generate functional artificial muscles by promoting host myogenic cell migration and differentiation, as well as nervous fibres, vascular networks, and satellite cell (SC) homing. However, acellular tissue mainly composed of extracellular matrix (ECM) allowed better myofibre three-dimensional (3D) organization and the restoration of SC pool, when compared to scaffolds which also preserved muscular cytoskeletal structures. Finally, we showed that fibroblasts are indispensable to promote efficient migration and myogenesis by muscle stem cells across the scaffolds in vitro. This data strongly support the use of xenogeneic acellular muscles as device to treat VML conditions in absence of donor cell implementation, as well as in vitro model for studying cell interplay during myogenesis.
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- 2018
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3. Publisher Correction: In Utero Gene Therapy (IUGT) Using GLOBE Lentiviral Vector Phenotypically Corrects the Heterozygous Humanised Mouse Model and Its Progress Can Be Monitored Using MRI Techniques
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Panicos Shangaris, Stavros P. Loukogeorgakis, Sindhu Subramaniam, Christina Flouri, Laurence H. Jackson, Wei Wang, Michael P. Blundell, Shanrun Liu, Simon Eaton, Nahla Bakhamis, Durrgah Latchumi Ramachandra, Panayiotis Maghsoudlou, Luca Urbani, Simon N. Waddington, Ayad Eddaoudi, Joy Archer, Michael N. Antoniou, Daniel J. Stuckey, Manfred Schmidt, Adrian J. Thrasher, Thomas M. Ryan, Paolo De Coppi, and Anna L. David
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Medicine ,Science - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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- 2019
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4. Mouse decellularised liver scaffold improves human embryonic and induced pluripotent stem cells differentiation into hepatocyte-like cells.
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Maëlle Lorvellec, Federico Scottoni, Claire Crowley, Rebeca Fiadeiro, Panagiotis Maghsoudlou, Alessandro Filippo Pellegata, Francesca Mazzacuva, Asllan Gjinovci, Anne-Marie Lyne, Justine Zulini, Daniel Little, Olukunbi Mosaku, Deirdre Kelly, Paolo De Coppi, and Paul Gissen
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Medicine ,Science - Abstract
Liver transplantation is the definitive treatment of liver failure but donor organ shortage limits its availability. Stem cells are highly expandable and have the potential to differentiate into any specialist cell. Use of patient-derived induced Pluripotent Stem Cells (hiPSCs) has the additional advantage for organ regeneration therapies by removing the need for immunosuppression. We compared hepatocyte differentiation of human embryonic stem cells (hESCs) and hiPSCs in a mouse decellularised liver scaffold (3D) with standard in vitro protocol (2D). Mouse livers were decellularised preserving micro-architecture, blood vessel network and extracellular matrix. hESCs and hiPSCs were primed towards the definitive endoderm. Cells were then seeded either in 3D or 2D cultures and the hepatocyte differentiation was continued. Both hESCs and hiPSCs differentiated more efficiently in 3D than in 2D, with higher and earlier expression of mature hepatocyte marker albumin, lipid and glycogen synthesis associated with a decrease in expression of fetal hepatocyte marker alpha-fetoprotein. Thus we conclude that stem cell hepatocyte differentiation in 3D culture promotes faster cell maturation. This finding suggests that optimised 3D protocols could allow generation of mature liver cells not achieved so far in standard 2D conditions and lead to improvement in cell models of liver disease and regenerative medicine applications.
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- 2017
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5. Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application.
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Luca Urbani, Panagiotis Maghsoudlou, Anna Milan, Maria Menikou, Charlotte Klara Hagen, Giorgia Totonelli, Carlotta Camilli, Simon Eaton, Alan Burns, Alessandro Olivo, and Paolo De Coppi
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Medicine ,Science - Abstract
Oesophageal tissue engineering is a therapeutic alternative when oesophageal replacement is required. Decellularised scaffolds are ideal as they are derived from tissue-specific extracellular matrix and are non-immunogenic. However, appropriate preservation may significantly affect scaffold behaviour. Here we aim to prove that an effective method for short- and long-term preservation can be applied to tissue engineered products allowing their translation to clinical application. Rabbit oesophagi were decellularised using the detergent-enzymatic treatment (DET), a combination of deionised water, sodium deoxycholate and DNase-I. Samples were stored in phosphate-buffered saline solution at 4°C (4°C) or slow cooled in medium with 10% Me2SO at -1°C/min followed by storage in liquid nitrogen (SCM). Structural and functional analyses were performed prior to and after 2 and 4 weeks and 3 and 6 months of storage under each condition. Efficient decellularisation was achieved after 2 cycles of DET as determined with histology and DNA quantification, with preservation of the ECM. Only the SCM method, commonly used for cell storage, maintained the architecture and biomechanical properties of the scaffold up to 6 months. On the contrary, 4°C method was effective for short-term storage but led to a progressive distortion and degradation of the tissue architecture at the following time points. Efficient storage allows a timely use of decellularised oesophagi, essential for clinical translation. Here we describe that slow cooling with cryoprotectant solution in liquid nitrogen vapour leads to reliable long-term storage of decellularised oesophageal scaffolds for tissue engineering purposes.
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- 2017
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6. Opium use during pregnancy and risk of preterm delivery: A population-based cohort study.
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Siavash Maghsoudlou, Sven Cnattingius, Scott Montgomery, Mohsen Aarabi, Shahriar Semnani, Anna-Karin Wikström, and Shahram Bahmanyar
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Medicine ,Science - Abstract
BACKGROUND:Use of narcotic or "recreational" drugs has been associated with adverse pregnancy outcomes such as preterm delivery. However, the associations might be confounded by other factors related to high-risk behaviours. This is the first study to investigate the association between traditional opium use during pregnancy and risk of preterm delivery. METHOD AND FINDINGS:We performed a population-based cohort study in the rural areas of the Golestan province, Iran between 2008 and 2010. We randomly selected 920 women who used (usually smoked) opium during pregnancy and 920 women who did not. Logistic regression models were used to estimate odds ratios (OR) and 95% confidence intervals (CI) for the associations between the opium use during pregnancy and preterm delivery and adjustment was made for potential confounding factors. This study shows compared with non-use of opium and tobacco, use of only opium during pregnancy was associated with an increased risk of preterm delivery (OR = 1.56; 95% CI 1.05-2.32), and the risk was more than two-fold increased among dual users of opium and tobacco (OR = 2.31; 95% CI 1.37-3.90). We observed that opium use only was associated with a doubled risk for preterm caesarean delivery (OR = 2.05; 95% CI 1.10-3.82) but not for preterm vaginal delivery (OR = 1.25; 95% CI 0.75-2.07). Dual use of opium and tobacco was associated with a substantially increased risk of vaginal preterm delivery (OR = 2.58; 95% CI 1.41-4.71). CONCLUSIONS:Opium use during pregnancy among non-tobacco smokers is associated with an increased risk of preterm caesarean delivery, indicating an increased risk of a compromised foetus before or during labour. Women who use both opium and smoked during pregnancy have an increased risk of preterm vaginal delivery, indicating an increased risk of spontaneous preterm delivery.
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- 2017
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7. Optimization of Liver Decellularization Maintains Extracellular Matrix Micro-Architecture and Composition Predisposing to Effective Cell Seeding.
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Panagiotis Maghsoudlou, Fanourios Georgiades, Holly Smith, Anna Milan, Panicos Shangaris, Luca Urbani, Stavros P Loukogeorgakis, Benedetta Lombardi, Giuseppe Mazza, Charlotte Hagen, Neil J Sebire, Mark Turmaine, Simon Eaton, Alessandro Olivo, Jasminka Godovac-Zimmermann, Massimo Pinzani, Paul Gissen, and Paolo De Coppi
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Medicine ,Science - Abstract
Hepatic tissue engineering using decellularized scaffolds is a potential therapeutic alternative to conventional transplantation. However, scaffolds are usually obtained using decellularization protocols that destroy the extracellular matrix (ECM) and hamper clinical translation. We aim to develop a decellularization technique that reliably maintains hepatic microarchitecture and ECM components. Isolated rat livers were decellularized by detergent-enzymatic technique with (EDTA-DET) or without EDTA (DET). Histology, DNA quantification and proteomics confirmed decellularization with further DNA reduction with the addition of EDTA. Quantification, histology, immunostaining, and proteomics demonstrated preservation of extracellular matrix components in both scaffolds with a higher amount of collagen and glycosaminoglycans in the EDTA-DET scaffold. Scanning electron microscopy and X-ray phase contrast imaging showed microarchitecture preservation, with EDTA-DET scaffolds more tightly packed. DET scaffold seeding with a hepatocellular cell line demonstrated complete repopulation in 14 days, with cells proliferating at that time. Decellularization using DET preserves microarchitecture and extracellular matrix components whilst allowing for cell growth for up to 14 days. Addition of EDTA creates a denser, more compact matrix. Transplantation of the scaffolds and scaling up of the methodology are the next steps for successful hepatic tissue engineering.
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- 2016
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8. Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors
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Urbani, Luca, Camilli, Carlotta, Phylactopoulos, Demetra-Ellie, Crowley, Claire, Natarajan, Dipa, Scottoni, Federico, Maghsoudlou, Panayiotis, McCann, Conor J., Pellegata, Alessandro Filippo, Urciuolo, Anna, Deguchi, Koichi, Khalaf, Sahira, Aruta, Salvatore Ferdinando, Signorelli, Maria Cristina, Kiely, David, Hannon, Edward, Trevisan, Matteo, Wong, Rui Rachel, Baradez, Marc Olivier, Moulding, Dale, Virasami, Alex, Gjinovci, Asllan, Loukogeorgakis, Stavros, Mantero, Sara, Thapar, Nikhil, Sebire, Neil, Eaton, Simon, Lowdell, Mark, Cossu, Giulio, Bonfanti, Paola, and De Coppi, Paolo
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Animals ,Cell Culture Techniques ,Cell Differentiation ,Child ,Child, Preschool ,Cryopreservation ,Epithelial Cells ,Esophagus ,Extracellular Matrix ,Humans ,Infant ,Infant, Newborn ,Male ,Mice, Inbred C57BL ,Mice, Transgenic ,Muscle, Skeletal ,Neural Crest ,Rats, Sprague-Dawley ,Tissue Engineering ,Tissue Scaffolds ,Genetics and Molecular Biology (all) ,ACELLULAR MATRIX ,MULTIPOTENT STEM-CELLS ,DETERGENT ENZYMATIC TREATMENT ,Inbred C57BL ,Biochemistry ,Transgenic ,Mice ,lcsh:Science ,Chemistry (all) ,Skeletal ,Multidisciplinary Sciences ,Muscle ,Science & Technology - Other Topics ,SKELETAL-MUSCLE ,Science ,SMALL-INTESTINAL SUBMUCOSA ,DOG-MODEL ,Article ,Physics and Astronomy (all) ,EXTRACELLULAR-MATRIX ,Preschool ,Science & Technology ,Newborn ,Rats ,CANINE MODEL ,BIOLOGIC SCAFFOLDS ,Biochemistry, Genetics and Molecular Biology (all) ,TISSUE ,lcsh:Q ,Sprague-Dawley - Abstract
A tissue engineered oesophagus could overcome limitations associated with oesophageal substitution. Combining decellularized scaffolds with patient-derived cells shows promise for regeneration of tissue defects. In this proof-of-principle study, a two-stage approach for generation of a bio-artificial oesophageal graft addresses some major challenges in organ engineering, namely: (i) development of multi-strata tubular structures, (ii) appropriate re-population/maturation of constructs before transplantation, (iii) cryopreservation of bio-engineered organs and (iv) in vivo pre-vascularization. The graft comprises decellularized rat oesophagus homogeneously re-populated with mesoangioblasts and fibroblasts for the muscle layer. The oesophageal muscle reaches organised maturation after dynamic culture in a bioreactor and functional integration with neural crest stem cells. Grafts are pre-vascularised in vivo in the omentum prior to mucosa reconstitution with expanded epithelial progenitors. Overall, our optimised two-stage approach produces a fully re-populated, structurally organized and pre-vascularized oesophageal substitute, which could become an alternative to current oesophageal substitutes., Combining decellularised scaffolds with patient-derived cells holds promise for bioengineering of functional tissues. Here the authors develop a two-stage approach to engineer an oesophageal graft that retains the structural organisation of native oesophagus.
- Published
- 2018
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