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2 results on '"Bissels, U"'

1. Delivery of RNA-based molecules to human hematopoietic stem and progenitor cells for modulation of gene expression.

Author

Diener Y, Bosio A, and Bissels U

Subjects
Genetic Therapy methods, Hematopoietic Stem Cell Transplantation, Humans, MicroRNAs chemistry, MicroRNAs genetics, RNA administration & dosage, RNA chemistry, RNA, Antisense chemistry, RNA, Messenger chemistry, RNA, Messenger genetics, Transfection methods, Cell Engineering, Gene Expression, Gene Transfer Techniques, Hematopoietic Stem Cells metabolism, RNA genetics
Abstract

Gene modulation of human hematopoietic stem and progenitor cells (HSPCs) harbors great potential for therapeutic application of these cells and presents a versatile tool in basic research to enhance our understanding of HSPC biology. However, stable genetic modification might be adverse, particularly in clinical settings. Here, we review a broad range of approaches to transient, nonviral modulation of protein expression with a focus on RNA-based methods. We compare different delivery methods and describe the usefulness of RNA molecules for overexpression as well as downregulation of proteins in HSPCs., (Copyright © 2016 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. All rights reserved.)

Published
2016
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2. A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing

Author

Astrid Novosel, Markus Landthaler, Julia Schliwka, Sabina Chiaretti, Miklós Palkovits, Roberto Di Lauro, Nicola Iovino, Mirabela Rusu, Roman-Ulrich Müller, Giuseppe Macino, James W. Nagle, Andreas Bosio, Mihaela Zavolan, Peter Wernet, Alice O. Kamphorst, Charles E. Rogler, Chris Sander, Veit Hornung, James J. Russo, Wayne Tam, Thomas Benzing, Alexei A. Aravin, Uta Fuchs, Thomas Tuschl, Pablo Landgraf, Sébastien Pfeffer, Alain Sewer, Hans Ingo Trompeter, Jason M. Inman, Ruchi Choksi, Amanda J. Rice, Arndt Borkhardt, Robert L. Sheridan, Valerio Fulci, Carolina Lin, Leandro C. Hermida, Michael J. Brownstein, Ute Bissels, Bernhard Schermer, Daniela Frezzetti, Minchen Chien, Grace Teng, Jingyue Ju, Quang Phan, Gunther Hartmann, F. Nina Papavasiliou, Robin Foà, Nicholas D. Socci, David B. Weir, Gabriella De Vita, Peter Lichter, Landgraf, P, Rusu, M, Sheridan, R, Sewer, A, Iovino, N, Aravin, A, Pfeffer, S, Rice, A, Kamphorst, Ao, Landthaler, M, Lin, C, Socci, Nd, Hermida, L, Fulci, V, Chiaretti, S, Foa, R, Schliwka, J, Fuchs, U, Novosel, A, Muller, Ru, Schermer, B, Bissels, U, Inman, J, Phan, Q, Chien, M, Weir, Db, Choksi, R, DE VITA, Gabriella, Frezzetti, D, Trompeter, Hi, Hornung, V, Teng, G, Hartmann, G, Palkovits, M, DI LAURO, Roberto, Wernet, P, Macino, G, Rogler, Ce, Nagle, Jw, Ju, J, Papavasiliou, Fn, Benzing, T, Lichter, P, Tam, W, Brownstein, Mj, Bosio, A, Borkhardt, A, Russo, Jj, Sander, C, Zavolan, M, Tuschl, T., Institut de biologie moléculaire des plantes (IBMP), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
Small RNA, moleneuro, molimmuno, rna, Molecular Sequence Data, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Genome, General Biochemistry, Genetics and Molecular Biology, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, IsomiR, Mirtron, Sequence Homology, Nucleic Acid, microRNA, Animals, Humans, Cell Lineage, RNA, Messenger, MOLIMMUNO, Conserved Sequence, Phylogeny, MOLENEURO, Gene Library, 030304 developmental biology, Genetics, Regulation of gene expression, 0303 health sciences, Base Sequence, Biochemistry, Genetics and Molecular Biology(all), Gene Expression Profiling, RNA, Hematopoietic Stem Cells, Rats, Gene expression profiling, MicroRNAs, Gene Expression Regulation, Hematologic Neoplasms, 030220 oncology & carcinogenesis
Abstract

SummaryMicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.

Published
2007

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