1. Monoclonal antibody labels both rod and cone outer segments of turtle photoreceptors.
- Author
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Ohtsuka T and Kawamata K
- Subjects
- Animals, Antibody Specificity, Cross Reactions, Epitopes analysis, Eye Proteins analysis, Eye Proteins immunology, Immunoenzyme Techniques, Retinal Pigments analysis, Rhodopsin analysis, Rhodopsin immunology, Rod Opsins, Antibodies, Monoclonal immunology, Photoreceptor Cells analysis, Retinal Pigments immunology, Rod Cell Outer Segment analysis, Turtles metabolism
- Abstract
We have studied the immunoreactivity of turtle photoreceptors to a monoclonal antibody (MAb 15-18) which binds to the external loop connecting bovine rhodopsin helices IV-V. Three chromatic types of cone photoreceptors were identified by the presence and color of oil droplets. MAb 15-18 intensely labeled the outer segments of both rods and green cones. In addition, a weak cross-reactivity was also found in the outer segments of red cones having a pale-green oil droplet, and of blue cones. Other morphological subtypes of red cones, cones with a red oil droplet and both members of double cones, showed no labeling. Our results indicate that rhodopsin and green cone opsin have a similar antigenic determinant, and that two different structural forms of red cone opsin may be present in the turtle retina.
- Published
- 1990
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