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Altered rhodopsin accessibility in the retinal dystrophic mouse.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1985 Oct 30; Vol. 132 (2), pp. 804-10. - Publication Year :
- 1985
-
Abstract
- Retinas obtained from 7-day-old rd mice show less reaction with antirhodopsin antisera than retinas from normal mice of the same age. Likewise, antisera prepared against synthetic peptides, which corresponds to the carboxyl terminus of rhodopsin, also react less with rd retinas from 7-day-old mice. In contrast, Western blots of denatured rhodopsin from rd vs. normal retinas of the same age indicate no change in the total quantity of this protein. These results demonstrate that in the 7-day-old rd mouse retina, rhodopsin is not altered in quantity; rather, it is less accessible to reaction with anti-rhodopsin antisera. Furthermore, these results suggest that the site of altered accessibility is on the carboxyl terminus of rhodopsin.
- Subjects :
- Age Factors
Animals
Calmodulin deficiency
Cyclic GMP metabolism
Immunologic Techniques
Mice
Retina analysis
Retinal Degeneration genetics
Rhodopsin immunology
Rod Cell Outer Segment metabolism
Mice, Mutant Strains metabolism
Retinal Degeneration metabolism
Retinal Pigments metabolism
Rhodopsin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 132
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 2998385
- Full Text :
- https://doi.org/10.1016/0006-291x(85)91203-3