1. Sequence-specific recognition of methylated DNA by human zinc-finger proteins.
- Author
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Sasai N, Nakao M, and Defossez PA
- Subjects
- Base Sequence, Binding Sites, Consensus Sequence, DNA-Binding Proteins chemistry, Humans, Protein Binding, Repressor Proteins chemistry, Transcription Factors metabolism, DNA Methylation, DNA-Binding Proteins metabolism, Repressor Proteins metabolism
- Abstract
DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achieved is largely unclear. Here, we report that the human zinc-finger proteins Kaiso, ZBTB4 and ZBTB38 can bind methylated DNA in a sequence-specific manner, and that they may use a mode of binding common to other zinc-finger proteins. This suggests that many other sequence-specific methyl binding proteins may exist.
- Published
- 2010
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