Crystallization and preliminary X-ray diffraction studies of a replication initiator protein (RepE54) of the mini-F plasmid complexed with iteron DNA.

Authors :: Komori H
Sasai N
Matsunaga F
Wada C
Miki K
Source :: Journal of biochemistry [J Biochem] 1999 Jan; Vol. 125 (1), pp. 24-6.
Publication Year :: 1999
Abstract: A replication initiator protein (RepE54) complexed with iteron DNA at its binding site was crystallized by the hanging drop vapor diffusion method. The crystals belong to monoclinic space group C2 with unit cell dimensions of a = 108.4 A, b = 81.9 A, c = 73.9 A, and beta = 121.5 degrees, where one molecule of the protein-DNA complex exists per asymmetric unit. They diffract X-rays up to 2.6 A resolution with synchrotron radiation.

Subjects :: Bacterial Proteins genetics
Bacterial Proteins metabolism
Crystallization
Crystallography, X-Ray
DNA-Binding Proteins genetics
Mutation
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Repetitive Sequences, Nucleic Acid
Repressor Proteins genetics
Bacterial Proteins chemistry
DNA chemistry
DNA-Binding Proteins chemistry
DNA-Binding Proteins metabolism
Escherichia coli Proteins
Nucleic Acid Heteroduplexes chemistry
Plasmids chemistry
Repressor Proteins chemistry
Repressor Proteins metabolism

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