1. A comparative analysis of recombinant protein expression in different biofactories: bacteria, insect cells and plant systems.
- Author
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Gecchele E, Merlin M, Brozzetti A, Falorni A, Pezzotti M, and Avesani L
- Subjects
- Animals, Baculoviridae genetics, Baculoviridae metabolism, Escherichia coli genetics, Escherichia coli metabolism, Glutamate Decarboxylase genetics, Humans, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, Recombinant Proteins genetics, Sf9 Cells virology, Spodoptera, Nicotiana enzymology, Biotechnology methods, Glutamate Decarboxylase biosynthesis, Recombinant Proteins biosynthesis, Nicotiana genetics, Nicotiana metabolism
- Abstract
Plant-based systems are considered a valuable platform for the production of recombinant proteins as a result of their well-documented potential for the flexible, low-cost production of high-quality, bioactive products. In this study, we compared the expression of a target human recombinant protein in traditional fermenter-based cell cultures (bacterial and insect) with plant-based expression systems, both transient and stable. For each platform, we described the set-up, optimization and length of the production process, the final product quality and the yields and we evaluated provisional production costs, specific for the selected target recombinant protein. Overall, our results indicate that bacteria are unsuitable for the production of the target protein due to its accumulation within insoluble inclusion bodies. On the other hand, plant-based systems are versatile platforms that allow the production of the selected protein at lower-costs than Baculovirus/insect cell system. In particular, stable transgenic lines displayed the highest-yield of the final product and transient expressing plants the fastest process development. However, not all recombinant proteins may benefit from plant-based systems but the best production platform should be determined empirically with a case-by-case approach, as described here.
- Published
- 2015
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