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A comparative analysis of recombinant protein expression in different biofactories: bacteria, insect cells and plant systems.
- Source :
-
Journal of visualized experiments : JoVE [J Vis Exp] 2015 Mar 23 (97). Date of Electronic Publication: 2015 Mar 23. - Publication Year :
- 2015
-
Abstract
- Plant-based systems are considered a valuable platform for the production of recombinant proteins as a result of their well-documented potential for the flexible, low-cost production of high-quality, bioactive products. In this study, we compared the expression of a target human recombinant protein in traditional fermenter-based cell cultures (bacterial and insect) with plant-based expression systems, both transient and stable. For each platform, we described the set-up, optimization and length of the production process, the final product quality and the yields and we evaluated provisional production costs, specific for the selected target recombinant protein. Overall, our results indicate that bacteria are unsuitable for the production of the target protein due to its accumulation within insoluble inclusion bodies. On the other hand, plant-based systems are versatile platforms that allow the production of the selected protein at lower-costs than Baculovirus/insect cell system. In particular, stable transgenic lines displayed the highest-yield of the final product and transient expressing plants the fastest process development. However, not all recombinant proteins may benefit from plant-based systems but the best production platform should be determined empirically with a case-by-case approach, as described here.
- Subjects :
- Animals
Baculoviridae genetics
Baculoviridae metabolism
Escherichia coli genetics
Escherichia coli metabolism
Glutamate Decarboxylase genetics
Humans
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Recombinant Proteins genetics
Sf9 Cells virology
Spodoptera
Nicotiana enzymology
Biotechnology methods
Glutamate Decarboxylase biosynthesis
Recombinant Proteins biosynthesis
Nicotiana genetics
Nicotiana metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-087X
- Issue :
- 97
- Database :
- MEDLINE
- Journal :
- Journal of visualized experiments : JoVE
- Publication Type :
- Academic Journal
- Accession number :
- 25867956
- Full Text :
- https://doi.org/10.3791/52459