1. Optogenetic mapping of feeding and self-stimulation within the lateral hypothalamus of the rat
- Author
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Kent C. Berridge and Kevin R. Urstadt
- Subjects
Food intake ,Lateral hypothalamus ,Physiology ,Channelrhodopsin ,Stimulation ,Biochemistry ,Eating ,Self Stimulation ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Animal Anatomy ,Mammals ,Neurons ,2. Zero hunger ,Brain Mapping ,0303 health sciences ,Multidisciplinary ,Chemistry ,05 social sciences ,Lateral preoptic area ,Eukaryota ,Animal Models ,Bioassays and Physiological Analysis ,Optical Equipment ,Experimental Organism Systems ,Laser intensity ,Vertebrates ,Medicine ,Engineering and Technology ,Eating behavior ,Cellular Types ,Anatomy ,Reward motivation ,Research Article ,Yellow Fluorescent Protein ,Science ,Equipment ,Surgical and Invasive Medical Procedures ,Optogenetics ,Biology ,Research and Analysis Methods ,Rodents ,050105 experimental psychology ,03 medical and health sciences ,Model Organisms ,Reward ,Animals ,0501 psychology and cognitive sciences ,030304 developmental biology ,Motivation ,Functional Electrical Stimulation ,Lasers ,Organisms ,Biology and Life Sciences ,Proteins ,Feeding Behavior ,Neurophysiological Analysis ,Cell Biology ,Preoptic Area ,Electric Stimulation ,Rats ,Luminescent Proteins ,Hypothalamic Area, Lateral ,Cellular Neuroscience ,Amniotes ,Animal Studies ,Physiological Processes ,Zoology ,Neuroscience ,030217 neurology & neurosurgery - Abstract
The lateral hypothalamus (LH) includes several anatomical subregions involved in eating and reward motivation. This study explored localization of function across different LH subregions in controlling food intake stimulated by optogenetic channelrhodopsin excitation, and in supporting laser self-stimulation. We particularly compared the tuberal LH subregion, the posterior LH subregion, and the lateral preoptic area. Local diameters of tissue optogenetically stimulated within the LH were assessed by measuring laser-induced Fos plumes and Jun plumes via immunofluorescence surrounding optic fiber tips. Those plume diameters were used to map localization of function for behavioral effects elicited by LH optogenetic stimulation. Optogenetic stimulation of the tuberal subsection of the LH produced the most robust eating behavior and food intake initially, but produced only mild laser self-stimulation in the same rats. However, after repeated exposures to optogenetic stimulation, tuberal LH behavioral profiles shifted toward more self-stimulation and less food intake. By contrast, stimulation of the lateral preoptic area produced relatively little food intake or self-stimulation, either initially or after extended stimulation experience. Stimulation in the posterior LH subregion supported moderate self-stimulation, but not food intake, and at higher laser intensity shifted valence to evoke escape behaviors. We conclude that the tuberal LH subregion may best mediate stimulation-bound increases in food intake stimulated by optogenetic excitation. However, incentive motivational effects of tuberal LH stimulation may shift toward self-stimulation behavior after repeated stimulation. By contrast, the lateral preoptic area and posterior LH do not as readily elicit either eating behavior or laser self-stimulation, and may be more prone to higher-intensity aversive effects.
- Published
- 2019
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