1. In vitro antimicrobial activity of a new ophthalmic solution containing povidone-iodine 0.6% (IODIM ® ).
- Author
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Pinna A, Donadu MG, Usai D, Dore S, D'Amico-Ricci G, Boscia F, and Zanetti S
- Subjects
- Candida isolation & purification, Colony Count, Microbial, Corneal Ulcer microbiology, Culture Media, Humans, Microbial Sensitivity Tests, Ophthalmic Solutions pharmacology, Pharmaceutical Preparations, Pseudomonas aeruginosa isolation & purification, Staphylococcus aureus isolation & purification, Staphylococcus epidermidis isolation & purification, Time Factors, Anti-Infective Agents, Local pharmacology, Candida drug effects, Povidone-Iodine pharmacology, Pseudomonas aeruginosa drug effects, Staphylococcus aureus drug effects, Staphylococcus epidermidis drug effects
- Abstract
Purpose: To assess the in vitro antimicrobial activity of a new commercial ophthalmic solution containing povidone-iodine 0.6% (IODIM
® )., Methods: Staphylococcus aureus ATCC 43300, Pseudomonas aeruginosa ATCC 27853, three ocular bacterial isolates (1 S. epidermidis, 1 S. aureus, 1 P. aeruginosa) and five Candida species were used. The bacterial and fungal isolates were cultured on Columbia blood agar base plates and Sabouraud-dextrose agar plates, respectively and incubated overnight at 37°C. Bacterial and fungal suspensions in sterile saline solution were prepared to an optical density equal to 0.5 McFarland standard (approximately 108 CFU/ml). Suspensions of the isolates were made in IODIM® solution to obtain a final concentration of 106 CFU/ml. The suspensions were then distributed in conical tubes in a final volume of 1 ml and incubated at 37°C. At different time-points (1, 5, 10, 15, 20, 25, 30 min and 24 hr), 10 μl of each suspension was removed, seeded on Columbia blood agar base and Sabouraud-dextrose agar plates and then incubated for 24 hr at 37°C. Positive and negative controls were included in all experiments., Results: After 5-min incubation, there was no bacterial growth on any plate. Conversely, IODIM® failed to kill the Candida isolates after 30 min' exposure and needed 24 hr to eradicate the organisms., Conclusion: IODIM® ophthalmic solution showed in vitro antimicrobial activity against S. epidermidis, S. aureus, P. aeruginosa and Candida species. Results suggest that it may be a potential candidate for the treatment of ocular surface infections and antimicrobial prophylaxis before intravitreal injections., (© 2019 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.)- Published
- 2020
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