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Susceptibility of various corneal fungal isolates and Pseudomonas aeruginosa to contact lens disinfecting solutions.
- Source :
-
Journal of infection in developing countries [J Infect Dev Ctries] 2013 Mar 14; Vol. 7 (3), pp. 261-8. Date of Electronic Publication: 2013 Mar 14. - Publication Year :
- 2013
-
Abstract
- Introduction: We aimed to investigate the susceptibility of a combined inoculum of Pseudomonas aeruginosa and different fungal strains to 6 soft contact lens disinfectants.<br />Methodology: One corneal isolate of P. aeruginosa and 13 corneal fungal isolates (9 Aspergillus spp, 3 Fusarium spp, 1 Curvularia sp.) were used. The following solutions were tested: Arion Cronos, Complete RevitaLens, Dua Elite, Opti-Free Express, Regard, Oxysept Comfort, and Oxysept Comfort without catalase. The effect of the solutions was assessed on a combined inoculum of P. aeruginosa plus 1 fungal strain. Suspensions of P. aeruginosa and fungi were made in the solutions (1x106 colony-forming units/mL). After 1 hour (Arion Cronos only), 6, 8, and 24 hours, aliquots of suspension were removed and seeded on Luria-Bertani and Sabouraud agar plates.<br />Results: After 6 hours' exposure, all the solutions but Dua Elite and Oxysept Comfort eradicated P. aeruginosa. Conversely, apart from 3% hydrogen peroxide-based Oxysept Comfort without catalase, which eradicated all the fungi tested after 6 hours, all the other solutions were partly ineffective at killing some of the fungal isolates, even after 24 hours' exposure.<br />Conclusions: Most contact lens disinfectants may be ineffective if contact lens care systems become co-contaminated with P. aeruginosa and fungi. In our experiment, only exposure to 3% hydrogen peroxide without neutralizer for at least 6 hours was always able to kill a combined inoculum of P. aeruginosa and different fungal strains.
- Subjects :
- Colony Count, Microbial
Fungi isolation & purification
Humans
Microbial Sensitivity Tests
Microbial Viability drug effects
Pseudomonas aeruginosa isolation & purification
Time Factors
Anti-Infective Agents pharmacology
Contact Lens Solutions pharmacology
Cornea microbiology
Fungi drug effects
Pseudomonas aeruginosa drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1972-2680
- Volume :
- 7
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of infection in developing countries
- Publication Type :
- Academic Journal
- Accession number :
- 23493005
- Full Text :
- https://doi.org/10.3855/jidc.2756