1. Virus-Enabled Biosensor for Human Serum Albumin.
- Author
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Ogata AF, Edgar JM, Majumdar S, Briggs JS, Patterson SV, Tan MX, Kudlacek ST, Schneider CA, Weiss GA, and Penner RM
- Subjects
- Biosensing Techniques methods, Electric Conductivity, Electric Impedance, Electrodes, Equipment Design, Humans, Limit of Detection, Reproducibility of Results, Serum Albumin, Human analysis, Bacteriophage M13 chemistry, Biosensing Techniques instrumentation, Bridged Bicyclo Compounds, Heterocyclic chemistry, Polymers chemistry, Serum Albumin, Human urine, Virion chemistry
- Abstract
The label-free detection of human serum albumin (HSA) in aqueous buffer is demonstrated using a simple, monolithic, two-electrode electrochemical biosensor. In this device, both millimeter-scale electrodes are coated with a thin layer of a composite containing M13 virus particles and the electronically conductive polymer poly(3,4-ethylenedioxy thiophene) or PEDOT. These virus particles, engineered to selectively bind HSA, serve as receptors in this biosensor. The resistance component of the electrical impedance, Z
re , measured between these two electrodes provides electrical transduction of HSA binding to the virus-PEDOT film. The analysis of sample volumes as small as 50 μL is made possible using a microfluidic cell. Upon exposure to HSA, virus-PEDOT films show a prompt increase in Zre within 5 s and a stable Zre signal within 15 min. HSA concentrations in the range from 100 nM to 5 μM are detectable. Sensor-to-sensor reproducibility of the HSA measurement is characterized by a coefficient-of-variance (COV) ranging from 2% to 8% across this entire concentration range. In addition, virus-PEDOT sensors successfully detected HSA in synthetic urine solutions.- Published
- 2017
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