Back to Search
Start Over
Virus-polymer hybrid nanowires tailored to detect prostate-specific membrane antigen.
- Source :
-
Analytical chemistry [Anal Chem] 2012 Mar 20; Vol. 84 (6), pp. 2776-83. Date of Electronic Publication: 2012 Mar 07. - Publication Year :
- 2012
-
Abstract
- We demonstrate the de novo fabrication of a biosensor, based upon virus-containing poly(3,4-ethylene-dioxythiophene) (PEDOT) nanowires, that detects prostate-specific membrane antigen (PSMA). This development process occurs in three phases: (1) isolation of a M13 virus with a displayed polypeptide receptor, from a library of ≈10(11) phage-displayed peptides, which binds PSMA with high affinity and selectivity, (2) microfabrication of PEDOT nanowires that entrain these virus particles using the lithographically patterned nanowire electrodeposition (LPNE) method, and (3) electrical detection of the PSMA in high ionic strength (150 mM salt) media, including synthetic urine, using an array of virus-PEDOT nanowires with the electrical resistance of these nanowires for transduction. The electrical resistance of an array of these nanowires increases linearly with the PSMA concentration from 20 to 120 nM in high ionic strength phosphate-buffered fluoride (PBF) buffer, yielding a limit of detection (LOD) for PSMA of 56 nM.
- Subjects :
- Amino Acid Sequence
Bacteriophage M13 isolation & purification
Equipment Design
Humans
Limit of Detection
Male
Peptide Library
Peptides chemistry
Peptides immunology
Prostatic Neoplasms immunology
Antigens, Surface analysis
Antigens, Surface immunology
Bacteriophage M13 immunology
Biosensing Techniques instrumentation
Bridged Bicyclo Compounds, Heterocyclic chemistry
Glutamate Carboxypeptidase II analysis
Glutamate Carboxypeptidase II immunology
Nanowires chemistry
Polymers chemistry
Prostatic Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 84
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22339784
- Full Text :
- https://doi.org/10.1021/ac203143y