1. Detection of tick-borne pathogens by MassTag polymerase chain reaction.
- Author
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Tokarz R, Kapoor V, Samuel JE, Bouyer DH, Briese T, and Lipkin WI
- Subjects
- Anaplasma isolation & purification, Animals, Borrelia isolation & purification, DNA Primers, Polymerase Chain Reaction methods, United States, Arachnid Vectors microbiology, Polymerase Chain Reaction veterinary, Ticks microbiology
- Abstract
MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.
- Published
- 2009
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