1. Immunization with a peptide derived from the G glycoprotein of bovine respiratory syncytial virus (BRSV) reduces the incidence of BRSV-associated pneumonia in the natural host.
- Author
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Bastien N, Taylor G, Thomas LH, Wyld SG, Simard C, and Trudel M
- Subjects
- Animals, Cattle, Immunization, Mice, Mice, Inbred BALB C, Pneumonia prevention & control, Viral Envelope Proteins, Cattle Diseases prevention & control, HN Protein, Peptide Fragments immunology, Pneumonia veterinary, Respiratory Syncytial Virus Infections veterinary, Respiratory Syncytial Virus, Bovine immunology, Viral Proteins immunology, Viral Vaccines immunology
- Abstract
Previous reports demonstrate that synthetic peptides corresponding to the amino acid region 174-187 of G glycoprotein from subgroups A and B human respiratory syncytial virus (HRSV), containing a Cys-->Ser substitution at position 186, confer complete resistance to immunized BALB/c mice against infection with the respective virus. In this report, we show that a Cys186-->Ser substituted peptide (BG/174-187) representing the corresponding region of the bovine (B) RSV G glycoprotein conferred complete protection of mice against BRSV challenge, suggesting that the 174-187 region of RSV G glycoproteins constitutes a dominant protective epitope which has been maintained throughout evolution. Furthermore, immunization of calves with peptide BG/174-187 efficiently induced the production of antibodies capable of recognizing both the parental G glycoprotein and peptide BG/174-187. Following challenge with live BRSV, although none of the animals were protected from upper respiratory tract disease, there were little or no gross pneumonic lesions in the four peptide-immunized calves. In contrast, moderate to extensive pneumonic lesions were observed in 2 out of 3 calves in the control group. Our results thus suggest that peptide BG/174-187 efficiently prevented BRSV-associated pneumonia in the natural host. The use of this system as a model is quite promising with regard to the development of a human synthetic vaccine.
- Published
- 1997
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