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Immunization with a peptide derived from the G glycoprotein of bovine respiratory syncytial virus (BRSV) reduces the incidence of BRSV-associated pneumonia in the natural host.
- Source :
-
Vaccine [Vaccine] 1997 Aug-Sep; Vol. 15 (12-13), pp. 1385-90. - Publication Year :
- 1997
-
Abstract
- Previous reports demonstrate that synthetic peptides corresponding to the amino acid region 174-187 of G glycoprotein from subgroups A and B human respiratory syncytial virus (HRSV), containing a Cys-->Ser substitution at position 186, confer complete resistance to immunized BALB/c mice against infection with the respective virus. In this report, we show that a Cys186-->Ser substituted peptide (BG/174-187) representing the corresponding region of the bovine (B) RSV G glycoprotein conferred complete protection of mice against BRSV challenge, suggesting that the 174-187 region of RSV G glycoproteins constitutes a dominant protective epitope which has been maintained throughout evolution. Furthermore, immunization of calves with peptide BG/174-187 efficiently induced the production of antibodies capable of recognizing both the parental G glycoprotein and peptide BG/174-187. Following challenge with live BRSV, although none of the animals were protected from upper respiratory tract disease, there were little or no gross pneumonic lesions in the four peptide-immunized calves. In contrast, moderate to extensive pneumonic lesions were observed in 2 out of 3 calves in the control group. Our results thus suggest that peptide BG/174-187 efficiently prevented BRSV-associated pneumonia in the natural host. The use of this system as a model is quite promising with regard to the development of a human synthetic vaccine.
- Subjects :
- Animals
Cattle
Immunization
Mice
Mice, Inbred BALB C
Pneumonia prevention & control
Viral Envelope Proteins
Cattle Diseases prevention & control
HN Protein
Peptide Fragments immunology
Pneumonia veterinary
Respiratory Syncytial Virus Infections veterinary
Respiratory Syncytial Virus, Bovine immunology
Viral Proteins immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 15
- Issue :
- 12-13
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 9302749
- Full Text :
- https://doi.org/10.1016/s0264-410x(97)00033-9