1. Protective Immunity in Mice Immunized With P. vivax MSP1 19 -Based Formulations and Challenged With P. berghei Expressing Pv MSP1 19 .
- Author
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Dobrescu I, de Camargo TM, Gimenez AM, Murillo O, Amorim KNDS, Marinho CRF, Soares IS, Boscardin SB, and Bargieri DY
- Subjects
- Animals, Antibodies, Protozoan immunology, Female, Malaria, Vivax parasitology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Parasitemia immunology, Plasmids genetics, Plasmodium berghei genetics, Protozoan Proteins immunology, Transfection, Treatment Outcome, Vaccination, Antigens, Protozoan immunology, Immunogenicity, Vaccine, Malaria Vaccines immunology, Malaria, Vivax immunology, Merozoite Surface Protein 1 immunology, Merozoite Surface Protein 1 metabolism, Plasmodium berghei metabolism, Plasmodium vivax immunology
- Abstract
The lack of continuous in vitro cultures has been an obstacle delaying pre-clinical testing of Plasmodium vivax vaccine formulations based on known antigens. In this study, we generated a model to test available formulations based on the P. vivax MSP1
19 antigen. The Plasmodium berghei strains ANKA and NK65 were modified to express Pv MSP119 instead of the endogenous Pb MSP119 . The hybrid parasites were used to challenge C57BL/6 or BALB/c mice immunized with Pv MSP119 -based vaccine formulations. The Pv MSP119 was correctly expressed in the P. berghei hybrid mutant lines as confirmed by immunofluorescence using anti- Pv MSP119 monoclonal antibodies and by Western blot. Replacement of the Pb MSP119 by the Pv MSP119 had no impact on asexual growth in vivo . High titers of specific antibodies to Pv MSP119 were not sufficient to control initial parasitemia in the immunized mice, but late parasitemia control and a balanced inflammatory process protected these mice from dying, suggesting that an established immune response to Pv MSP119 in this model can help immunity mounted later during infection., (Copyright © 2020 Dobrescu, de Camargo, Gimenez, Murillo, Amorim, Marinho, Soares, Boscardin and Bargieri.)- Published
- 2020
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