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Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection.
- Source :
-
Microbes and infection [Microbes Infect] 2008 Oct; Vol. 10 (12-13), pp. 1266-73. Date of Electronic Publication: 2008 Jul 22. - Publication Year :
- 2008
-
Abstract
- The Apical Membrane Antigen-1 (AMA-1) of Plasmodium sp. has been suggested as a vaccine candidate against malaria. This protein seems to be involved in merozoite invasion and its extra-cellular portion contains three distinct domains: DI, DII, and DIII. Previously, we described that Plasmodium vivax AMA-1 (PvAMA-1) ectodomain is highly immunogenic in natural human infections. Here, we expressed each domain, separately or in combination (DI-II or DII-III), as bacterial recombinant proteins to map immunodominant epitopes within the PvAMA-1 ectodomain. IgG recognition was assessed by ELISA using sera of P. vivax-infected individuals collected from endemic regions of Brazil or antibodies raised in immunized mice. The frequencies of responders to recombinant proteins containing the DII were higher than the others and similar to the ones observed against the PvAMA-1 ectodomain. Moreover, ELISA inhibition assays using the PvAMA-1 ectodomain as substrate revealed the presence of many common epitopes within DI-II that are recognized by human immune antibodies. Finally, immunization of mice with the PvAMA-1 ectodomain induced high levels of antibodies predominantly to DI-II. Together, our results indicate that DII is particularly immunogenic during natural human infections, thus indicating that this region could be used as part of an experimental sub-unit vaccine to prevent vivax malaria.
- Subjects :
- Animals
Antibodies, Protozoan blood
Antibodies, Protozoan immunology
Antigens, Protozoan genetics
Female
Humans
Immunization
Immunoglobulin G blood
Immunoglobulin G immunology
Malaria, Vivax parasitology
Malaria, Vivax prevention & control
Membrane Proteins genetics
Mice
Mice, Inbred BALB C
Protozoan Proteins genetics
Protozoan Vaccines administration & dosage
Protozoan Vaccines immunology
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins immunology
Antigens, Protozoan chemistry
Antigens, Protozoan immunology
Immune Sera immunology
Malaria, Vivax immunology
Membrane Proteins chemistry
Membrane Proteins immunology
Plasmodium vivax immunology
Protozoan Proteins chemistry
Protozoan Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1286-4579
- Volume :
- 10
- Issue :
- 12-13
- Database :
- MEDLINE
- Journal :
- Microbes and infection
- Publication Type :
- Academic Journal
- Accession number :
- 18692152
- Full Text :
- https://doi.org/10.1016/j.micinf.2008.07.023