1. Therapeutic assessment of fractions of Gastrodiae Rhizoma on chronic atrophic gastritis by 1 H NMR-based metabolomics.
- Author
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Xu H, Ruan LY, Chen C, Fan JT, Chen JF, Zhao WL, Meng HH, Liu WY, Zhao WL, Zheng Q, Liu ZC, and Wang JS
- Subjects
- Animals, Hydrophobic and Hydrophilic Interactions, Male, Plant Extracts chemistry, Proton Magnetic Resonance Spectroscopy, Rats, Rhizome chemistry, Solvents chemistry, Gastritis, Atrophic prevention & control, Gastrodia chemistry, Metabolomics, Plant Extracts pharmacology
- Abstract
Ethnopharmacological Relevance: Gastrodiae Rhizoma (GR), a well-known and commonly-used TCM (Traditional Chinese Medicine) for treating headache, dizziness, tetanus, epilepsy, and etc., has been proven to relieve chronic atrophic gastritis (CAG). Due to its complex ingredients, the active fractions responsible for the treatment of CAG remain largely unknown., Aim of the Study: To explore the underlying material and interpret its underlying mechanism, the therapeutic effect of extract from different polar parts of Gastrodiae Rhizoma on autoimmune CAG was studied based on the
1 H NMR metabolomics., Materials and Methods: The rat model of CAG was established by autoimmune method. The modeled CAG rats were then treated with 4 polar parts (T1-4 in descending polarity, corresponding to water, n-butanol, ethyl acetate and petroleum ether extracts, respectively) of Gastrodiae Rhizoma for 21 consecutive days. The stomach and serum samples were collected and then subjected to histopathology observation, biochemical measurement (MDA, SOD, GSH, NO, XOD and pepsin),1 H NMR metabolic profiling and multivariate/univariate statistical analysis., Results: The results showed that T1 had the best therapeutic effect, T2 the second, and T3 and T4 the poorest with no obvious therapeutic effect, demonstrating that the effective components of Gastrodiae Rhizoma should be compounds of high polarity. T1 achieved good therapeutic effects due to the anti-inflammatory and anti-oxidant activities, and by rectifying the disturbed energy and amino acid metabolism in CAG model., Conclusion: This integrated metabolomics approach proved the validity of the therapeutic effect of extract from different polar parts of Gastrodiae Rhizoma on autoimmune CAG, providing new insights into the underlying mechanisms, and demonstrating the feasibility of metabolomics to evaluate efficacy of herbal drug, which is often difficult by traditional means., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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