1. Peroxisomal NAD(H) Homeostasis in the Yeast Debaryomyces hansenii Depends on Two Redox Shuttles and the NAD + Carrier, Pmp47.
- Author
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Turkolmez S, Chornyi S, Alhajouj S, IJlst L, Waterham HR, Mitchell PJ, Hettema EH, and van Roermund CWT
- Subjects
- Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae genetics, Debaryomyces metabolism, Debaryomyces genetics, Fatty Acids metabolism, Fungal Proteins metabolism, Fungal Proteins genetics, Membrane Proteins metabolism, Membrane Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Saccharomyces cerevisiae Proteins genetics, Peroxisomes metabolism, Oxidation-Reduction, NAD metabolism, Homeostasis
- Abstract
Debaryomyces hansenii is considered an unconventional yeast with a strong biotechnological potential, which can produce and store high amounts of lipids. However, relatively little is known about its lipid metabolism, and genetic tools for this yeast have been limited. The aim of this study was to explore the fatty acid β-oxidation pathway in D. hansenii . To this end, we employed recently developed methods to generate multiple gene deletions and tag open reading frames with GFP in their chromosomal context in this yeast. We found that, similar as in other yeasts, the β-oxidation of fatty acids in D. hansenii was restricted to peroxisomes. We report a series of experiments in D. hansenii and the well-studied yeast Saccharomyces cerevisiae that show that the homeostasis of NAD
+ in D. hansenii peroxisomes is dependent upon the peroxisomal membrane protein Pmp47 and two peroxisomal dehydrogenases, Mdh3 and Gpd1, which both export reducing equivalents produced during β-oxidation to the cytosol. Pmp47 is the first identified NAD+ carrier in yeast peroxisomes.- Published
- 2023
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