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Yeast peroxisomes multiply by growth and division.
- Source :
-
The Journal of cell biology [J Cell Biol] 2007 Jul 30; Vol. 178 (3), pp. 399-410. Date of Electronic Publication: 2007 Jul 23. - Publication Year :
- 2007
-
Abstract
- Peroxisomes can arise de novo from the endoplasmic reticulum (ER) via a maturation process. Peroxisomes can also multiply by fission. We have investigated how these modes of multiplication contribute to peroxisome numbers in Saccharomyces cerevisiae and the role of the dynamin-related proteins (Drps) in these processes. We have developed pulse-chase and mating assays to follow the fate of existing peroxisomes, de novo-formed peroxisomes, and ER-derived preperoxisomal structures. We find that in wild-type (WT) cells, peroxisomes multiply by fission and do not form de novo. A marker for the maturation pathway, Pex3-GFP, is delivered from the ER to existing peroxisomes. Strikingly, cells lacking peroxisomes as a result of a segregation defect do form peroxisomes de novo. This process is slower than peroxisome multiplication in WT cells and is Drp independent. In contrast, peroxisome fission is Drp dependent. Our results show that peroxisomes multiply by growth and division under our assay conditions. We conclude that the ER to peroxisome pathway functions to supply existing peroxisomes with essential membrane constituents.
- Subjects :
- Dynamins metabolism
Endoplasmic Reticulum metabolism
GTP Phosphohydrolases genetics
GTP Phosphohydrolases metabolism
GTP-Binding Proteins genetics
GTP-Binding Proteins metabolism
Membrane Proteins genetics
Membrane Proteins metabolism
Mitochondrial Proteins
Peroxins
Peroxisomes ultrastructure
Protein Transport
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins genetics
Saccharomyces cerevisiae Proteins metabolism
Vesicular Transport Proteins
Peroxisomes metabolism
Saccharomyces cerevisiae cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 178
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 17646399
- Full Text :
- https://doi.org/10.1083/jcb.200702167