Search

Ethnopharmacological Relevance: The traditional Chinese herb Panax notoginseng (PN) tonifies blood, and its main active ingredient is saponin. PN is processed by different methods, resulting in different compositions and effects., Aim of the Study: To investigate changes in the microstructure and composition of fresh PN processed by different techniques and the anti-anemia effects on tumor-bearing BALB/c mice after chemotherapy with cyclophosphamide (CTX)., Materials and Methods: Fresh PN was processed by hot-air drying (raw PN, RPN), steamed at 120 °C for 5 h (steamed PN, SPN), or fried at 130 °C, 160 °C, or 200 °C for 8 min (fried PN, FPN1, FPN2, or FPN3, respectively); then, the microstructures were compared with 3D optical microscopy, quasi-targeted metabolites were detected by liquid chromatography tandem mass spectrometry (LC‒MS/MS), and saponins were detected by high-performance liquid chromatography (HPLC). An anemic mouse model was established by subcutaneous H22 cell injection and treatment with CTX. The antianemia effects of PN after processing via three methods were investigated by measuring peripheral blood parameters, performing HE staining and measuring cell proliferation via immunofluorescence., Results: 3D optical profiling revealed that the surface roughness of the SPN and FPN was greater than that of the other materials. Quasi-targeted metabolomics revealed that SPN and FPN had more differentially abundant metabolites whose abundance increased, while SPN had greater amounts of terpenoids and flavones. Analysis of the composition and content of the targeted saponins revealed that the contents of rare saponins (ginsenoside Rh 1 , 20(S)-Rg 3 , 20(R)-Rg 3 , Rh 4 , Rk 3 , Rg 5 ) were greater in the SPN. In animal experiments, the RBC, WBC, HGB and HCT levels in peripheral blood were increased by SPN and FPN. HE staining and immunofluorescence showed that H-SPN and M-FPN promoted bone marrow and spleen cell proliferation., Conclusion: The microstructure and components of fresh PN differed after processing via different methods. SPN and FPN ameliorated CTX-induced anemia in mice, but the effects of PN processed by these two methods did not differ., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

The mechanism of how potassium (K) attenuates cadmium (Cd)-induced demethylation and anabolism of cell wall (CW) pectin through the brassinolide (BR) signaling pathway was verified in Panax notoginseng (Burk.). The P. notoginseng pectin methylesterase gene (PnPME1) was cloned and functionally verified in tobacco. Pectin and BR metabolism, Cd content and the pectin methylation degree (PMD) were detected in response to K, 2,4-epibrassinolide (EBL), and brassinazole treatments of P. notoginseng and tobacco under Cd stress. Activity of the main root pectin methylesterase enzyme (PME) was promoted by 22.29% under the EBL treatment, and Cd content increased by 29.03% under Cd stress. Potassium reduced PME activity and Cd content in main root pectin by 61.03% and 50.73%, respectively, under the EBL and Cd co-treatment. Potassium inhibited the promoting effects of Cd stress on the expression of PnPME1 by 57.04%. Potassium also inhibited expression of BR synthesis genes PnDET2, PnROT3, PnCYP90A1, and PnBR6OX1 by 65.61%, 52.02%, 47.36%, and 55.16%, respectively, and reduced the accumulation of Cd. The PnPME1 was located in the CW. The activity of transgenic tobacco root PME was higher than that of the wild-type, while the PMD was significantly lower. The regulatory effects of K and EBL on tobacco root pectin metabolism were consistent with those in P. notoginseng. In conclusion, K downregulated the expression of BR synthesis genes in P. notoginseng roots under Cd stress and reduced the production of BRs, which inhibited PnPME1 expression. The reduction in PME activity increased the PMD, which reduced the accumulation of Cd., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

The high background value of cadmium (Cd) in the Panax notoginseng planting soil is the main reason for the Cd content in P. notoginseng exceeding the limit standards. The main goal of this study was to reveal the mechanism by which potassium (K) reduces Cd accumulation in P. notoginseng from the perspective of the influences of soil microbial communities on soil pH, total organic matter (TOM) and cation exchange capacity (CEC). Pot experiments were conducted to study the effects of different types and amounts of applied K on the Cd content in P. notoginseng , and on the soil pH, TOM, CEC, and bioavailable Cd (bio-Cd) content in soil. Field experiments were conducted to study the effects of K2SO4 fertilizer on the microbial community, and its correlations with the soil pH, TOM and CEC were analyzed. A moderate application of K2SO4 (0.6 g⋅kg–1) was found to be the most optimal treatment for the reduction of Cd in the pot experiments. The field experiments proved that K fertilizer (K2SO4) alleviated the decreases in pH, TOM and CEC, and reduced the content of bio-Cd in the soil. The application of K fertilizer inhibited the growth of Acidobacteria, but the abundances of Mortierellomycota, Proteobacteria and Bacteroidetes were promoted. The relative abundances of Acidobacteria and Proteobacteria in the soil bacteria exhibited significant negative and positive correlations with pH and CEC, respectively. In contrast, the relative abundance of Mortierellomycota was found to be positively correlated with the pH, TOM and CEC. The bio-Cd content was also found to be positively correlated with the relative abundance of Acidobacteriia but negatively correlated with the relative abundances of Proteobacteria and Mortierellomycota. The application of K fertilizer inhibited the abundance of Acidobacteria, which alleviated the acidification of the soil pH and CEC, and promoted increase in the abundances of Mortierellomycota, Proteobacteria and Bacteroidetes, which ultimately increased the soil TOM and CEC. Soil microorganisms were found to mitigated decreases in the soil pH, TOM, and CEC and reduced the bio-Cd content in the soil, which significantly reduced the accumulation of Cd in P. notoginseng. [ABSTRACT FROM AUTHOR]

This study investigated the effect of nitrogen (N) and potassium (K) formula fertilization on cadmium (Cd) accumulation in P. notoginseng. Field investigations as well as formulated N and K fertilizers application experiments were conducted. Field investigations showed that Cd accumulation decreased PNS content in the main roots of P. notoginseng, while PNS content was promoted by soil available potassium (AK) and K in the main roots. The Cd content in P. notoginseng and the bioavailable Cd content in the soil decreased with the increasing of total K (TK) and AK in the soil. The increase of soil pH, total organic matter (TOM) and cation exchange capacity (CEC) values can reduce the bioavailable Cd content in soil, thus reducing the Cd accumulation in P. notoginseng. Under current fertilization in P. notoginseng cultivation, decreased N fertilization can alleviate the deterioration of soil physical and chemical properties. Under identical N fertilization, increasing K fertilization promoted the PNS accumulation (0.3–38.3%), also improved soil physical and chemical properties. Formulated N and K fertilizers application (1:2) experiments showed that reducing application of N and increasing K fertilization could reduce the bioavailable Cd content in soil, and the Cd content also decreased by 0.5–69.6% in P. notoginseng. Abbreviation: PNS: P. notoginseng saponins; F(EXC): Exchangeable fraction; F(Carb): Bound to carbonates fraction; F(Fe-MnOX): Bound to iron and manganese oxides fraction; F(OM): Bound to organic matter fraction; F(RES): Residual fraction; AK: Available potassium; TK: Total potassium; CEC: Cation exchange capacity; TOM: Total organic matter [ABSTRACT FROM AUTHOR]